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Validated All-in-One™ qPCR Primer for IL7(NM_000880.3) Search again
Product ID:
HQP009676
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IL-7
Gene Description:
interleukin 7
Target Gene Accession:
NM_000880.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. [provided by RefSeq].
Gene References into function
- IL7 downregulates both TGF-beta production and signaling in pulmonary fibroblasts.
- Effects on human thymus function.
- an aromatic residue is required at position 143 of human IL-7 for IL-7R binding and subsequent signal transduction
- increased HIV replication in the thymus by inducing differentiation and expansion of mature CD27(+) thymocytes expressing CXCR4 or CCR5
- IL-7 may function to regulate the milieu of the microenvironment by modulating IL-6 secretion by the IL-7R-expressing stromal elements
- IL-7 has a role in T cell regeneration that may be useful in enhancing immunity in AIDS infection [review]
- Patients with untreated AML had decreased IL-7 serum levels. Patients in complete remission had intermediate levels and developed adverse effects. IL-7 enhanced in vitro proliferation by clone T-cells and these cells responded to IL-2 and Il-7.
- C-terminal modification alters biological activity
- A role for IL-7-driven inflammation in atherogenesis was suggested and the promotion of clinical instability in coronary artery disease involving interactions between platelets, monocytes, and chemokines
- IL-7 and its downstream signalling complex have roles in some human solid malignancies [review]
- IL-7 regulates homeostasis by modulating the equilibrium between proliferation and apoptotic cell death in T cells that have recently exited from the thymus as well as mature naive and memory T cell subsets.
- IL-7 confers potent survival signals to some but not all subpopulations of human thymocytes in vitro; in contrast, in vivo administration of IL-7 to SCID/hu Thy/Liv mice is not associated with enhanced thymocyte survival or accelerated HIV infection.
- results confirm a role for SDF-1alpha and IL-7 in HIV-1 disease progression, and suggest that these cytokines play a role in the modulation of CXCR4
- Recombinant human IL-7 induces both a renewal and an expansion of T lymphocytes associated with cell activation in SIVmac-infected macaques without modulation of the other hemopoietic cells and with no increase in the viral load in blood or lymph nodes.
- the drop in CD4 in HIV children would induce an increase of IL-7 as part of a homeostatic mechanism
- Interaction between IL-7 and its receptor has the major role in modulating T-ALL survival within the microenvironment generated by the T-ALL/TEC interaction.
- Human IL-7 has potent effects on the survival and expansion of T cells that are sufficient to significantly increase lethality of murine graft-vs-host disease in the absence of a conditioning regimen.
- IL-7 induced the death of DN STAT5 expressing 697 cells occurs through caspase-dependent and -independent mechanisms that both require mitochondrial activation.
- Interleukin-7 and transforming growth factor-beta play counter-regulatory roles in protein kinase C-delta-dependent control of fibroblast collagen synthesis in pulmonary fibrosis
- Results suggest that the functional interplay between interferon regulatory factors 1 and 2 serves as an elaborate and cooperative mechanism for regulation of interleukin-7 production essential for local immune regulation within human intestinal mucosa.
- Interleukin-7 has a role in rejuvenating the immune system [review]
- interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells requires PI3 kinase
- elevated plasma levels of circulating IL-7 in a subgroup of common variable immunodeficiency
- IL-7 response to T-cell depletion may enhance T-cell production, but at the same time may foster HIV-1 disease progression favoring the emergence of more virulent HIV-1 strains characterized by syncytium-inducing capability and rapid replication rate.
- Transfected into bone marrow stromal cells, protects mice from lukemia following allogeneic T-cell-depleted bone marrow transplantation.
- Anergy induction by IL-7 and restoration of responsiveness by IL-15 suggest novel mechanisms for regulation of helper T-cell responses, induction of peripheral tolerance, and breakdown of T-cell self-tolerance.
- IL-7, which is increased endogenously in HIV-1-infected individuals late in disease, may be involved in the neuronal apoptosis
- findings suggest that higher IL-7 levels may contribute to higher CD4 counts in Hiv-1 infected women
- IL-7 produced by skin cells contributes to the survival and proliferation of T cells within skin lesions and is likely the source of elevated circulating IL-7 in CTCL.
- IL-7 plasma levels were higher in centenarian females than males.
- IL-7 promotes the extended survival of both naive and memory CD4+ T cells, whereas cycling of these two subsets is distinct and transient.
- IL-7 plays a major role in the expansion of mature T-cells that occurs during lymphopenia and has a role in immunotherapy [review]
- CD4+ T-cell lymphopenia has an impact on human B-cell development either directly or indirectly via the associated elevation of IL-7 levels
- IL-7 has a distinct inductive effect on APOBEC3G (A3G) deoxycytidine deaminase gene expression and A3G complex assembly that occur in natural cellular targets of human immunodeficiency virus infection.
- The level of IL-7-mediated reduction of apoptosis was inversely correlated with the number of circulating CD4+ T cells. The antiapoptotic effect of IL-7 was uncoupled from the induction of cellular proliferation or endogenous HIV-1 replication
- IL-7 has a major role in the enhanced survival mediated by BM stroma both in T-ALL cells and thymocytes
- IL-7 and, to a limited extent, TNFalpha, both of which are produced by activated monocytes and were detected in synovial fluid, abrogated the CD4+,CD25+ Treg-mediated suppression.
- A three base ATC deletion just upstream of an out-of-frame ATG codon in the upstream non-coding region of the IL-7 gene, reduces the efficiency of translation from the upstream, out-of-frame ATG.
- IL-7 levels were found to be strongly associated with ovarian cancer.
- During sleep and especially during late sleep serum interleukin-7 concentrations were distinctly increased as compared to wakefulness.
- IL-7 stimulates osteoclastogenesis by inducing TNF-alpha release by T and B cells
- Survival cytokines IL-7 and IL-15 are differentially involved in the growth and maintenance of heart-infiltrating peripheral CD8-positive T cells from patients with chronic Chagas disease cardiomyopathy.
- IL-7 produced by MS-5 cells is required for human pro-B-cell development from CD34(+)bone marrow cells in our culture system, and IL-7 appears to play a certain role in early human B lymphopoiesis.
- Activation of the IL-7 pathway may play an important role in lymphoid neogenesis in rheumatoid arthritis synovial tissue.
- Immunolocalization of IL-7, IL-7Ralpha, SDF-1alpha, and CXCR4 resulted in a diffuse but specific labeling. RT-PCR analysis confirmed the expression of the above-mentioned transcripts.
- Stimulation of the IL-7 pathway begins with IL-7 binding to unglycosylated and glycosylated forms of its alpha-receptor, IL-7 receptor alpha.
- The IL7 gene is very unlikely to influence the genetic susceptibility to MS in this population.
- IL-7 reduced CD127-surface expression and shedding by CD8+ T cells; results support a role for IL-7 in the down-regulation of CD127 expression and impairment of CTL function observed in HIV infection
- IL-7 is an important factor in the development of GVHD, presumably by supporting the survival, proliferation, and possibly activation of alloreactive donor-derived T cells in the recipients.
- IL-7 may contribute to cartilage destruction in joint diseases, including osteoarthritis.
- Levels of induced telomerase activity in cultured CD4-positive T cells are inversely correlated with cell death.
- data show that IL-7- and TCR/CD28-mediated signaling differentially regulate IL-7Ralpha expression on human T cells with a transient and chronic effect, respectively.
- HCV co-infection does not affect the TREC/IL-7 pathway in HIV disease.
- Data show that T cell loss after islet transplantation in patients with autoimmune type 1 diabetes was associated with both increased serum concentrations of IL-7 and IL-15 and in vivo proliferation of memory CD45RO(+) T cells.
- high IL-7 levels associated with lymphopenic conditions may simultaneously induce sensitivity to Fas-mediated apoptosis in nonactivated T cells and increase Fas-induced costimulatory signals in T cells recognizing low-affinity antigens.
- Expressed as soluble recombinant protein in Sf9 cells via nonlysing vector transfection, its secreted levels were 1.7 microg x 1(-1) under serum-free cell culture conditions.
- Data support preclinical observations that IL-7 plays a critical role in inducing acute graft-versus-host-disease.
- The SCID mutations of IL-7Ralpha locate outside the binding interface with IL-7, suggesting that the expressed mutations cause protein folding defects in IL-7Ralpha