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Validated All-in-One™ qPCR Primer for IL6ST(NM_002184.3) Search again
Product ID:
HQP009674
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD130, CDW130, GP130, HIES4, HIES4A, HIES4B, IL-6RB, IMD94, STWS2, sGP130
Gene Description:
interleukin 6 cytokine family signal transducer
Target Gene Accession:
NM_002184.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
Gene References into function
- Vav interacts with gp130.
- detection in pericardial fluid in coronary pathologies
- regulation of surface expression by janus kinase 1
- Shedding of the interleukin-6 (IL-6) receptor (gp80) determines the ability of IL-6 to induce gp130 phosphorylation in human osteoblasts
- Gp130 and ras mediated signaling in human plasma cell line INA-6: a cytokine-regulated tumor model for plasmacytoma.
- The transcription co-repressor TLE1 interacted with the intracellular region of gpl30 through its Q domain
- gp130 signal transduction is important for the differentiation and maturation of dendritic cells
- Soluble gp130 is up-regulated in the implantation window and shows altered secretion in patients with primary unexplained infertility.
- Diminished GP130 Abundance in congestive heart failure
- PKC delta associates with IL6ST via Stat3 and enhances Stat3-gp130 interaction
- Gp130 activation by soluble IL-6 receptors and IL-6 enhances osteoblastic differfentiation of human bone marrow-derived mesenchymal cells.
- interaction with cyclin-dependent kinase 9
- There is no significant effect of IL-18 on the release of both soluble receptors of IL-6.
- interactions of CNTFR with LIFR and gp130 in vitro
- Epidermal growth factor receptor-independent constitutive activation of STAT3 in head and neck squamous cell carcinoma is mediated by the autocrine/paracrine stimulation of the interleukin 6/gp130 cytokine system.
- Data suggest that interleukin-6 activation of gp130 promoted cell division in fibroblasts from patients with idiopathic pulmonary fibrosis (IPF), while IL-11 was mitogenic in both normal and IPF cells.
- a structural model, derived from 3.65 A resolution crystallographic data of the complex between IL-6, the extracellular binding domains of IL-6R, and the extracellular activation and binding domains of gp130 is presented
- involvement of IL-6 in the pathogenesis of liver diseases and suggest a protective role of IL-6/gp130-dependent pathways in nonparenchymal liver cells during fibrosis progression in chronic liver diseases.
- gp130 has a role in glucose-enhanced interleukin-6-induced VEGF165 expression
- CD30 can modify eosinophil survival by causing an extremely rapid and intense induction of apoptosis through a tightly regulated intracellular signaling pathway.
- GP130 together with the cytokine receptor WSX-1 constitutes a functional signal-transducing receptor for IL-27.
- Results suggest that the balance between soluble and membrane-bound gp130 may play an important role in regulating cytokine action necessary for blastocyst implantation and for further interaction between the decidualized endometrium and the trophoblast.
- Two distinct Stat3 signaling pathways emanating from gp130 are utilized in mammary tissue.
- LMO4 interaction modulates the interleukin-6 receptor subunit glycoprotein 130 complex and its signaling
- Endotoxemia up-regulates gp130 expression in vivo and in vitro.
- There is no evidence that mutations in exon 17 of the gp130 gene are involved in the pathogenesis of human inflammatory bowel disease
- RT-PCR analysis showed that glioma tumor cells express oncostatin M receptors.
- A crystal structure of the ligand-binding domains of gp130 in complex with human interleukin-6 (IL-6) and its a-receptor (IL-6Ralpha).
- urokinase-type plasminogen activator induces upregulated expression of the complement anaphylatoxin C5a receptor; this effect is mediated via the interaction of the uPA-specific receptor and gp130
- The function of IL-6R in the luminal and glandular epithelium might be different from that in the stroma during the implantation period.
- Simultaneous action of two IL-6 binding domains on two gp130 molecules is required to efficiently recruit a fluorescent IL-6 (yellow fluorescent protein-IL-6) to the plasma membrane.
- These findings indicate that the di-leucine motif which directs the internalization of the IL-6 receptor complex also mediates the basolateral sorting of the signal transducer gp130.
- there are tissue-specific differences in IL-6-receptor-gp130-coupled signaling which limit the extent of Stat3 activation and gammaFBG expression during lung inflammation
- decreased expression of GP130 in ejaculated spermatozoa could be associated with low sperm motility in asthenozoospermic men.
- demonstrates the existence of preformed but inactive gp130/leukemia inhibitory factor receptor hetero- and gp130/gp130 homo-dimers
- metastatic melanoma cells could escape this growth control by the epigenetic silencing of oncostatin M receptor beta
- physical evidence of the stabilization of vIL-6-induced gp130 signaling complexes by gp80
- sOSMR is able to bind OSM and interleukin-31 when associated to soluble gp130 or soluble interleukin-31R, respectively, and to neutralize both cytokine properties
- activation of RankL gene expression by PKA- and gp130-inducers is mediated via common regulatory domains that also served to facilitate the activity of 1,25-(OH)2D3
- potential role of gp130 receptor ligands as part of a therapeutic strategy to treat obesity.
- administration of IL-6 could activate receptor gp80/gp130 signaling pathways including downstream extracellular signal-regulated kinase 1/2 and STAT3 phosphorylation in EPCs.
- The expression of IL-6 and IL-6R (gp80, gp130) was studied by confocal immunofluorescence, rtPCR and Western blotting. Lipopolysaccharide (LPS) stimulation experiments were conducted in smooth muscle cell cultures of bladder biopsies of tumor patients.
- A higher gene expression of CRP, IL-6, and both IL-6 membrane receptors in subcutaneous samples of inflamed patients than in healthy controls.
- gp130 modulates atherosclerosis in mice and human
- gp130 protein expression (P < 0.05) and p42/44 MAPK and PI3K/Akt activation (P < 0.01) were decreased in heart-failure hypertensive patients compared with nonheart-failure hypertensive individuals.
- IL-6/sIL-6R complex trans-signaling via gp130 is an exacerbating factor of ACD, and that the concentration of sIL-6R in tears is a useful clinical biomarker in patients with ACD.
- SHP2-mediated signaling cascade is essential for the LIF and IL-6+sIL-6r-dependent increase in I(CaL), [Ca(2+)](i) transient and action potential duration.
- Increased expression of some IL-6 cytokine family members (oncostatin M, gp130, CT-1, LIF) in cutaneous inflammation might contribute to the promotion of hair loss.
- EGFR could activate the gp130/JAK/STAT3 pathway by means of IL-6 upregulation in primary human lung adenocarcinomas, making this pathway a potential target for cancer treatment.
- IL6ST variants are possible determinants of impaired glucose metabolism and other abnormalities of metabolic syndrome.
- we highlight the key role of the gp130 receptor and its major downstream effectors in the heart in terms of development and regeneration--REVIEW
- Active expression of transgenic Stat6 in IL-4-deficient mouse lymphocytes alters lymphocyte homeostasis and promotes T helper (Th) type 2 cell differentiation in vitro.
- Increasing the level of G{130 may assist in reducing the inflammatory changes induced by IL-6 transsignaling in uveitis aqueous humor.
- The lysosomal degradation of gp130 is critical for cessation of IL-6-mediated signaling.
- Knockdown of IL6ST (lentiviral infection with shRNA)in HEK293 cells expressing AblPP constitutively is associated with enhanced cell attachment.
- IL-6 treatment induced MUC4 expression through the gp130/STAT3 pathway, indicating the direct role of IL-6 on the activation of the intestinal mucin gene MUC4 in gastric cancer cells
- Astrocytic gp130 expression is crucial for survival of transgenic glial fibrillary acid protein (GFAP)-positive astrocytes in murine Toxoplasma encephalitis (TE). Astrocyte loss results in a lethal course of TE.
- Results present the biophysical and structural characterization of the full-length, transmembrane form of a quaternary cytokine receptor complex consisting of gp130, LIF-R, Ciliary Neurotrophic Factor (CNTF), and its alpha receptor (CNTF-Ralpha).
- increased ratio of sgp130/sIL-6R production and/or reduced sIL-6R production combined with down-regulation of IL-6R and SOCS-3 expression in trophoblasts may lead to less cytokine inhibitory activity in preeclampsia placentas
- recurrent gain-of-function gp130 mutations in these human hepatocellular adenomas fully explains activation of the acute inflammatory phase observed in tumourous hepatocytes