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Validated All-in-One™ qPCR Primer for IL5(NM_000879.2) Search again
Product ID:
HQP009666
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EDF, IL-5, TRF
Gene Description:
interleukin 5
Target Gene Accession:
NM_000879.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF).
Gene References into function
- inhibition of signaling by antisense oligodeoxynucleotides targeting the common beta chain of receptors
- data indicate that ability to produce the type 2 cytokines IL-13 and IL-5 defines CD161(+) NK cells at intermediate stages of differentiation, and is lost upon terminal functional differentiation, concomitant with acquired ability to produce IFN-gamma
- CD4(+) T cells are the major source of IL-5 among CD3(+) lymphocytes in atopic asthmatic subjects, whereas in nonatopic asthmatic subjects CD8 (+) T cells as well as CD4(+) T cells contribute to the increased production of IL-5
- By itself, IL5 increases nerve growth factor level in eosinophils, but in combination with immune complexes, significantly reduces eosinophil NGF levels.
- in asthma Il-5 might directly induce bronchial hyperresponsiveness
- A putative Bcl6-binding silencer element has been identified in the 3' untranslated region of IL-5 cDNA.
- the AP-1 complex plays a key role in regulating IL5 expression- this provides an explanation for the sensitivity of IL5 to protein synthesis inhibitors and a mechanism for the specific induction of IL5 compared with other cytokines.
- expression of mRNA for IL-4, IL-5, TGF-beta1, IFN-gamma in patients with cicatricial pemphigoid
- Constitutive overexpression of human IL-5 in transgenic mice induces migration of bone marrow progenitor cells to the spleen and extramedullary hematopoiesis in the spleen.
- IL-5 down-modulates its receptor via a proteinase-mediated process.
- Protease allergen Der p1 and the secreted proteases of the hookworm Necator americanus are able to directly induce type 2 cytokines IL-4, IL-5, and IL-13 by basophils, that could promote IgE synthesis, eosinophil recruitment, and Th2 cells.
- Increased IL-5 expression in muscle of eosinophilic myositis patients correlates with the presence of activated eosinophilic granules and the release of eosinophilic major basic protein in muscle biopsy specimens.
- This is the first evidence of an association between the IL5 gene polymorphism and bronchial asthma
- Incubation of eosinophils with IL-5 leads to reduced expression of IL-5R alpha, which is sustained for up to 5 days; eosinophil IL-3R alpha expression is increased by IL-5, whereas GM-CSF receptor alpha expression is not affected by IL-5.
- IL-5 gene transcription in human asthmatic peripheral T cells in vivo clearly demonstrate that an interaction with monocytes enhances IL-5 gene transcription.
- In all three groups of patients significant correlation was seen between abundance of IL-2 vs. IL-4, IL-5, IL-10, or TNF-alpha, between IL-4 vs. IL-10, and between TNF-alpha vs. INF-gamma
- Results show that the expression of interleukin-5 mRNA in mild asthma differed from that in severe and moderate asthma before and after corticosteroid treatment.
- IL-5 and eotaxin are associated with acute exacerbation of asthma.
- Hematopoietin receptor superfamily. Comprised of cytokine-specific alpha chain and common beta chain for signaling. Contributes to differentiation and function of leukocytes. Protective immunity and pathophysiology of immunologic diseases. Review.
- IL5 gene may play a role in blood eosinophilia associated with atopic dermatitis
- Results identify several transcriptional targets of interleukin-5 and granulocyte macrophage-colony-stimulating factor in human eosinophils and suggest that a number of protein products are critical to the responsiveness of airway eosinophils.
- skin mast cells precultured in the presence of IL-4 [alone or plus stem cell factor (SCF)] before anti-IgE stimulation, acquired the ability to produce IL-5, and IL-1beta was concomitantly suppressed
- Significantly higher levels were found in soluble egg antigen-stimulated PBMC from schistosomiasis mansoni patients with degree III hepatic fibrosis compared to patients with degree I or II fibrosis, and in patients untreated for 1 year.
- histone acetyltransferase (HAT) activity of CBP/p300 was required to activate IL-5 expression
- exposure of human mast cells to IL-4, IL-5, and IFN-gamma during growth and differentiation generally down-regulated mast cell number and function
- IL-5 induces the protein expression of cyclin D3 and the kinase Pim-1, both of which are regulated by STAT-dependent processes in airway and blood eosinophils.
- IL-5 acts as a triggering factor in the toxiallergic complaints commonly seen in helminth and protozoon infections.
- Up-regulated by a conserved upstream enhancer with two potential GATA-3-binding sites
- extracellular signal-regulated kinase-mediated adhesion of beta(2)-integrin caused by IL-5 is mediated in human eosinophils by a class IA PI3K through activation of a PKCdelta pathway
- analysis of IL5 induction of activation of the multisubunit receptor system using an interleukin-5 mimetic peptide
- interleukin-5 transcription repression by the glucocorticoid receptor targets GATA3 signaling and involves histone deacetylase recruitment
- Allergen-specific T cell lines induced in the presence of salmeterol and fluticasone proprionate inhibited IL-5 and IL-13 production by allergen-specific Th2 cell lines in an IL-10-dependent manner
- Interleukin 5 plays a role in colonic carcinogenesis from ulcerative colitis by interacting with IGF-II.
- Polymorphisms in the IL5 gene were associated with each other in whites and African Americans.
- This Th2 cytokine is possibly involved in the modulation of lung graft transplantation tolerance.
- These data suggest that during a respiratory virus infection activated CD8+ T cells from asthmatic subjects may produce excess type 2 cytokines and may contribute to asthma exacerbation by augmenting allergic inflammation.
- Eosinophils from asthmatics release IL-5 in an autocrine fashion to act on their own IL-5 receptors in prevention of apoptosis through the upregulation of Bcl-2 expression.
- Sputum IL-13, but not IL-5, is inversely correlated with the provocative concentration of a substance causing a 20% fall in FEV1 for methacholine in asthmatic patients
- The conserved lymphokine element 0 is important for inducible interleukin-5 transcription.
- dynamin has roles in the IL-5 signaling pathway and in receptor endocytosis and termination of the ERK1/2 signaling pathway
- ROC analysis demonstrated that the ratio of IL-5/IL-4 was the most sensitive parameter associated with acute graft-versus-host disease.
- serum expression of eotaxin & IL-5 were significantly increased in patients with strongyloidiasis compared with controls; this rise suggests that selective mediators of the eosinophil can have a role in immunity against S. stercoralis in human infection
- HDAC4 (histone deacetylase 4) and p300, a known HAT (histone acetyltransferase), reversibly controlled the activity of the IL-5 promoter in vivo and in vitro, with a concurrent alteration of histone H3 acetylation status at the promoter regions.
- study of link between SWA-specific Th2 cytokines IL4 and IL5 responsiveness and subsequent increases in worm-IgE, and age factors
- Ragweed stimulation significantly increased the production of the Th2-associated cytokines IL-5, IL-9 and IL-13, the chemokines CCL17 and CCL22 and the regulatory cytokine IL-10 in allergic patients
- Inhaled IL-5 modulated eosinophil progenitor numbers in both the airways and bone marrow of asthmatics and induced local eosinophilia in non-asthmatics.
- A significant correlation exists between bronchoalveolar lavage concentration of IL-5 and eosinophils in veterans with sulfur mustard gas-induced pulmonary fibrosis.
- ST2 reduced production of IL-4, IL-5, and IL-13 from IL-33-stimulated splenocytes. These results indicate that soluble ST2 acts as a negative regulator of Th2 cytokine production by the IL-33 signaling.
- IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence.
- Chromatin immunoprecipitation showed increased binding of Ets1 and GATA-3 to the IL-5 promoter after stimulation. Ets1, GATA-3 and AP-1 synergize to regulate IL-5 transcription in human T cells.
- Glucocorticoid receptor beta is able to act as a transcriptional repressor of cytokine genes, IL5 and IL13 and mediates its function through the recruitment of histone deacetylase complexes.
- IL-5 may modulate the immune response in individuals with symptomatic Schistosomiasis japonica.
- thrombin and cell interaction with lung epithelial cells may augment the inflammatory response in allergic diseases by stimulating the secretion of IL-5 from basophils
- ability of CD8hi CD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin
- IL-5 overexpression is associated with eosinophilia in T-cell lymphomas.
- The production of Th1 and Th2 cytokines by naive CD4+ T cells to mycobacterial antigen-pulsed DCs in the group of young, healthy BCG vaccinated volunteers, was investigated.
- Alterations of MMP-2 and -9 expression may play a role in eosinophilic chronic rhinosinusitis, but the association with IL-5 and IL-13 remains unclear
- cyclic AMP signals enhance histone H3 acetylation at the IL-5 promoter and the concerted binding of GATA-3 and NFATc to the promoter.
- Analysis of IL-5 expression suggests that several cytokines could participate in the physiopathology of EE in humans.IL-5 was not detected in control samples, and expression levels were variably downregulated after steroid treatment in six patients.
- Diffuse large B-cell lymphoma and follicular lymphoma grade 3 is more influenced by expression of B-cell differentiation stage genes than by tumor cells growth pattern, BCL2 and BCL6 abnormalities, and International Prognostic Index.
- Children with the higher IL-10/IL-5 ratios appeared to have lower risks for virtually all of the four categories of respiratory infections in each of the five 1-year periods spanning the study.