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Validated All-in-One™ qPCR Primer for IL4R(NM_000418.3) Search again
Product ID:
HQP009664
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD124, IL-4RA, IL4RA
Gene Description:
interleukin 4 receptor
Target Gene Accession:
NM_000418.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
Gene References into function
- gene-gene interaction in asthma: IL4RA and IL13 in a Dutch population with asthma
- Tyrosine-phosphorylated IL4R peptides coprecipitate SH2-containing tyrosine phosphatase-1, SH2-containing tyrosine phosphatase-2, and SH2-containing inositol 5'-phosphatase, demonstrating a regulatory role for the ITIM motif in IL-4-induced proliferation
- high-affinity interaction of human IL-4 and the receptor alpha chain is constituted by two independent binding clusters
- internalization of interleukin 4 receptor alpha increases cytotoxic effect of interleukin 4-receptor-targeted cytotoxin in cancer cells.
- Characterization of IL-4 receptor components expressed on monocytes and monocyte-derived macrophages: variation associated with differential signaling by IL-4
- These results suggest TXA2 receptor polymorphism strongly interacts with IL-4R alpha polymorphism as a major determinant of high serum immunoglobulin E levels in atopic dermatitis.
- Endogenous interferon-alpha production by differentiating human monocytes regulates expression and function of the IL-2/IL-4 receptor gamma chain
- a single gene effect of IL4Ralpha variants or any other gene on chromosome 16 could not be shown in a selected population of children with asthma
- no evidence for linkage of the IL4R gene locus with sarcoidosis
- association of haplotype with type 1 diabetes
- up-regulated 5-fold in B cell chronic lymphocytic leukemia cells, probably leading to increased responsiveness to IL4 and resistance to apoptosis
- role of Tyr-713 in the interleukin-4 receptor alpha in regulating dephosphorylation of Stat6
- Allele frequency of IL4RA polymorphism was associated with rapid decline of lung function in smokers.
- IL4R is associated with diabetes mellitus, type 1 in Filipinos.
- Th2 cytokines enhance TARC expression in human airway smooth muscle cells in IL-4Ralpha genotype-dependent fashion.
- No genotypic effects on total serum IgE levels.
- IL-4R alpha chain 576R/R genotypes confer genetic susceptibility to allergic asthma in Chinese.
- The prevalence of the mutant variant of the IL-4ra gene was lower in neonates with necrotizing enterocolitis (NEC) compared with those without NEC, suggesting that this mutation might protect against the development of NEC in VLBW infants.
- functional variants within the IL4R gene predispose to hip osteoarthritis in Caucasian females
- investigated the frequency and genotypes of S503P and Q576R SNPs and their association with traits of allergic asthma in a Hawaii population
- polymorphisms of IL1A (G/T at +4845) and IL4RA (T/C at +22446) show an epistatic effect on the risk of atopy
- variants in the IL4, IL13, and IL4RA genes play an important role in controlling specific IgE response in atopy
- Certain interstitial pneumonia patients can be modulated in a manner that is dependent on the IL-4 and IL-13 receptor subunit expression by these cells.
- V50R551 IL-4R alpha variant has enhanced function alone, but with Q110 IL-13 variant the 2 have a synergistic effect on IL-13-dependent gene induction.
- Polymorphisms of IL-4R previously associated with other immune mediated diseases, do not confer susceptibility to Graves' disease in white Caucasians in the United Kingdom.
- differences in the potency of IL-13- and IL-4-mediated induction of eotaxin-3 might be explained by expression of types 1 and 2 IL-4 receptors in bronchial epithelium
- The IL-4RalphaQ576R polymorphism may involve in the development of penicillins allergy, and through modulating specific serum IgE levels.
- IL$R alpha V50 homozygosity associates with slow progression and that exon 12 U-haplotypes might be associated with both susceptibility to infection via parenteral route and resistance to infection via sexual exposure.
- The soluble receptor balance (sIL-2R/sIL-4R) in patients with severe hemolytic uremic syndrome may shift, depending on the disease state of the patients
- In conclusion, these results suggest that viral airway infection may enhance interleukin-4-induced eotaxin-3 production through upregulation of the interleukin-4 receptor in airway epithelial cells.
- No association between type 1 diabetes and any SNP or haplotype was found by the transmission disequilibrium test.
- This is the first demonstration of sex-specific association of the two foremost genes of the IL-4 signalling cascade with chronic inflammatory arthropathies.
- Multifactor dimensionality reduction (MDR) test was applied to detect epistasis, and identified single-IL4R(Q576R)- and three-IL4R(Q576R), IL5RA(-80), CD14(-260)- locus association models that predict multiple sclerosis risk with 75-76% accuracy.
- A structure/function analysis of the IL-4 ligand-receptor interaction, using various mutations of both the ligand and the receptor, is reported.
- Results show sequence variations as a possible way of altering alternative splicing selection of IL4R in vivo.
- Homozygotes with the low activity allele of the A398G polymorphism in the IL4R gene had a modest increase in risk of atrophic gastritis (OR = 1.52, 95% CI: 1.05-2.21), compared with homozygotes of the high activity allele.
- Variants in the IL4RA gene alone may not exert any major influence on susceptibility to asthma-related diseases in childhood, but in combination with other genes, such as IL9R, IL4RA may be an important gene for disease susceptibility
- Our findings of higher frequency of IL4 and IL4RA genotypes and alleles with rheumatoid arthritis.
- SNPs in IL4Ralpha, which are more common in African Americans, are associated with severe asthma exacerbations, lower lung function, and increased mast cell-related tissue inflammation
- IL4+33 and IL4R*Q551 polymorphisms may have a promoting role of TH2 mediators in African MS descendants. IL4 and IL4R genes are susceptibility factors for Brazilian MS but may be able to modify ethnicity-dependent disease risk and penetrance.
- IL4 and IL4R polymorphisms and haplotypes were neither significantly associated with IgE levels in controls nor associated with glioma status
- A meta-analysis of results from case-control studies strongly supports the conclusion that the R551 IL4R variant imparts a modest yet significant risk for atopic asthma
- The IL-4R 75V variant was associated with increased risk of cervical tumors, cases homozygote for 75V had an odds ratio of 1.91 (1.27-2.86) with a tendency that the association was stronger in noncarriers of the DQB1*0602 allele.
- IL4R gene polymorphisms may have roles in asthma in Chinese populations
- analysis of polymorphisms of Ile50Val (rs.1805010), Ser478Pro (rs.1805015), and Gln551Arg (rs.1801275) in the IL4R gene between Japanese patients with Stevens-Johnson syndrome and Japanese healthy volunteers
- IL-4R overexpression on newborns' monocytes and lymphocytes could be an early risk marker of allergy development.
- The IL-4R Ile50/Ile50 and IL-10R2 G520/G520 and G520/A520 genotypes were shown to determine the susceptibility to SLE(systemic lupus erythematosus)in a Chinese population
- we studied the association of variants in IL-4 (C-589T, G3017T) and IL-4R alpha (Gln576Arg) with allergies and with risk of pancreatic cancer
- IL-4 and IL-4RA gene polymorphisms concur in selecting the H. pylori infecting strain, probably influencing the IL-4 signalling pathway
- combined polymorphisms in the IL-13/IL-4R signaling pathway were associated with SJS/TEN with ocular surface complications.
- Analyses of genotype distributions and allele frequencies of study subjects revealed that rs 180275 polymorphism in IL4R was associated with an increase in BMI in Korean population.
- our data indicate that the AA genotype of the IL4R 150V SNP is associated with joint erosions in psoriatic arthritis
- There is a lack of association or interactions between the IL-4, IL-4Ralpha and IL-13 genes, and rheumatoid arthritis.
- This study demonstrated upregulation of type 1 IL-4R by IFN-gamma, which resulted in enhanced IL-4-induced production of CCL26 from keratinocytes.
- Single nucleotide polymorphisms of IL4RA are not associated with olive pollen allergy or asthma, but the interaction between IL4RA I50V/Q551R was strongly associated with the asthma phenotype.
- Polymorphisms in the interleukin-4 receptor gene are associated with better survival in patients with glioblastoma
- data indicate inactivation of receptor-associated protein tyrosine phosphatase activity by cytokine-generated reactive oxygen species is a physiologic mechanism for amplification of IL-4 receptor activation revealing a role for ROS in cytokine crosstalk
- IL-4 activates signaling pathways through type I IL-4Rs qualitatively differently from IL-13, which cooperate to induce optimal gene expression.