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Validated All-in-One™ qPCR Primer for CCN1(NM_001554.4) Search again
Product ID:
HQP009564
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CYR61, GIG1, IGFBP10
Gene Description:
cellular communication network factor 1
Target Gene Accession:
NM_001554.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene.
Gene References into function
- Elevated levels of connective tissue growth factor, WISP-1, and CYR61 in primary breast cancers associated with more advanced features.
- Organization and expression of the Cyr61 gene in normal human fibroblasts. In serum-stimulated fibroblasts, an alternative RNA containing an in-frame deletion within exon 4 which disrupts the thrombospondin type 1 repeat was detected.
- promotes tumor growth; increased Cyr61 expression is associated with an aggressive phenotype of breast cancer cells
- Identification of integrin alpha(M)beta(2) as an adhesion receptor on human peripheral blood monocytes for Cyr61 and connective tissue growth factor: immediate-early gene products expressed in atherosclerotic lesions.
- Cyr61 is an early marker of extracellular matrix remodeling in rat bladder cells
- role in promoting cell survival and tubule formation in human umbilical vein endothelial cells
- in addition to its proangiogenic activity, Cyr61 may be a novel mediator of progesterone activity in enhancing growth-factor-driven tumor growth in breast cancer.
- Cyr61 promotes breast tumorigenesis and cancer progression
- CYR61 has an integrin alphaMbeta2 binding site mediating monocyte adhesion
- these findings define the T1 sequence in CCN1 as a novel binding motif for integrin alpha 6 beta 1
- Regulates angiogenesis and endothelial cell function. (review)
- The expression of the CYR61 gene depends on the physiologic state of G-actin monomers
- Cyr61 is a hypoxia-inducible angiogenesis factor in malignant melanoma with tumor stage-dependent expression
- Lysophosphatidic acids induce CYR61 by activating G protein-coupled receptors. CYR61 acts as secreted autocrine and/or paracrine mediator in stromal and epithelial hyperplasia. Potential importance of this signaling mechanism in the disease.
- CYR61 and CTGF may play a role in the progression of gliomas; their levels at diagnosis may have prognostic significance.
- Cyr61 plays an important role in resistance to chemotherapeutic agent-induced apoptosis in human breast cancer MCF-7 cells by a mechanism involving the activation of the integrins/NF-kappaB/XIAP signaling pathway
- Results suggest that Cyr61 may represent a gene characteristic for endometriosis and may play an important role in the development and persistence of endometriotic lesions.
- evidence that adenylate cyclase as well as one or several protein kinases might be involved in the mechanoregulation of Cyr61, CTGF and Nov genes
- Cyr61 activated the beta-catenin/TCF4 complex, which promoted the expression of c-myc and the latter induced expression of p53.
- serum growth factors induce the cysteine rich angiogenic inducer 61(CYR61) gene in both prostate stromal and epithelial cells, and CYR61 plays functional roles in cell adhesion, morphology, and proliferation
- Cyr61 has a role in suppressing growth of human endometrial cancer cells
- Cyr61 and CTGF genes may play an important role in hepatocellular carcinogenesis and correlate with recurrence and metastasis of hepatocellular carcinoma
- new role for cyr61 in HeLa cells infected with Coxsackievirus B3, which is associated with the cell death induced by virus infection
- CYR61 is sufficient to promote breast cancer cell proliferation, cell survival, and Taxol resistance.
- Cyr61 gene expression is significantly downregulated in the tumors of hepatocellular carcinoma patients.
- Recombinant cyr61 was expressed as a fusion protein with the Fc-domain of human IgG and purified using affinity chromatography on protein G-Sepharose columns. rCyr61 stimulated blood vessel formation and cell proliferation.
- Cyr61 expression provides cytoprotection in hyperoxia-induced pulmonary epithelial cell death and that this effect was in part mediated via the Akt signaling pathway
- Results suggest that CCN1 may play a role in ovarian carcinogenesis by stimulating survival and antiapoptotic signaling pathways.
- CYR61 is regulated by the Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells.
- In early-onset pre-eclamptic placentae, CYR61 was expressed at a significantly lower level compared with normal matched controls, indicating a potential role of this molecule in the pathogenesis of early-onset pre-eclampsia.
- The 3 most significantly overexpressed genes in rheumatoid arthritis were laeverin, 11beta-hydroxysteroid dehydrogenase type 2 (a steroid pathway enzyme), and cysteine-rich, angiogenic inducer 61 (a known angiogenic factor).
- These data identify CYR61 as a pivotal regulator of collagen production and degradation in aged and photoaged human skin.
- IGFBP-3, IGFBP-4, and IGFBP-10/CYR61 mRNA levels were up-regulated in Barrett's and tumour tissue of oesophageal adenocarcinoma patients
- Experiments using a recombinant mutant human CYR61 promoter-directed reporter gene expression confirmed transcriptional regulation of CYR61 by FOXO3a.
- CCN1 promotes integrin-dependent recruitment of CD34+ progenitor cells to endothelial cells, which may contribute to paracrine effects on angiogenesis and tissue regeneration.
- We found significant differences in gene expression, specifically with a group of genes, including CYR61, known to be responsive to estrogen or to interact with estrogen receptor.
- In normal endometrium Cyr61 expression is highest in proliferatory phase; it is overexpressed in midsecretory phase in polycystic ovary syndrome (PCOS) and endometrial cancer.
- VEGF-mediated up-regulation of CCN1 in osteoblasts that attracts endothelial cells and promotes angiogenesis. Such a loop could be operative during fracture healing.
- Exercise with high mechanical loading induced the expression of Cyr61 in human skeletal muscle.
- CCN1 is expressed in podocytes, acts on glomerular cells to modulate glomerular remodeling, and is downregulated in diseased kidneys. Impairment of CCN1 expression in podocytes may contribute to progression of glomerular disease with mesangial expansion.
- In men & women, rs 3753794 & rs 3753793 (r2 = 0.77) were associated plasma HDL-cholesterol levels. Carriers of A allele for rs 3753794 were more likely to have high plasma HDL-cholesterol levels as compared with G/G homozygotes & A/A homozygotes.
- Blocking experiments with the lysophosphatidic acid (LPA) receptor-specific inhibitor Ki16425 suggest a general involvement of LPA receptors in bacteria-triggered CYR61 expression.
- CCN1 promotes growth in glioma cells, CCN1-overexpressing C6 cells. In addition to its effect on cell growth, CCN1 also increased the motility of glioma cells in the presence of extracellular substrates such as fibronectin.
- Cyr61 promotes interleukin-8-dependent chemotaxis, transendothelial migration, and intravasation by induction of CXCR1/CXCR2 through integrin alphavbeta3/Src/PI3K/Akt-dependent pathway.
- Cyr61 appears to guard against metastatic disease because low expression is associated with more advanced disease; and therefore, expression levels of Cyr61 correlate with the prognosis of lung cancer.
- Reduction of CCN1/CCN3 in preeclampsia could be responsible for the failure of uterine vascular remodeling.
- The opposite effect of TNFalpha combined with hypoxia on CYR61 up-regulation could contribute to a balanced expression level of this angiogenic factor in the endometrium.
- Increased expression of this protein correlates with the loss of VHL protein expression in renal cell carcinoma.
- Cyr61 promoted gastric cancer cell invasive ability via an HIF-1alpha-dependent up-regulation of PAI-1.
- The multimodular structure of CCN1 enables it to fulfill multiple functions that may contribute to the different stages of cancer development.
- SP exposure to human intestinal microvascular endothelial cells co-cultured with NCM460-NK-1R cells induced angiogenic activity that was inhibited by CCN1 silencing.
- Thrombin receptor and RhoA mediate cell proliferation through integrins and cysteine-rich protein 61.
- CYR61 prevents cisplatin-induced apoptosis by inhibiting caspase-3 activity in HeLa cells.
- Overexpression of CCN1 mediated by CREB is enhanced in hypoxic regions of tumors.
- Cyr61 played an important role in the tumorigenicity and metastasis of PCa cells.
- Cyr61 is highly expressed in some endometrial cancer of endometrioid subtype. Cyr61 expression and positive lymph node metastases were independent prognostic factors for patients with endometrioid adenocarcinoma.
- The sphingosine-1-phosphate receptor signaling enhances invasiveness in glioblastoma, partially through CCN1.
- hypoxia serves as a specific inducer of alternative Cyr61 splicing toward the intron skipping mRNA isoform with potential biological consequences in tumor cells.