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Validated All-in-One™ qPCR Primer for IFNA2(NM_000605.3) Search again
Product ID:
HQP009422
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IFN-alpha-2, IFN-alphaA, IFNA, IFNA2B, leIF A
Gene Description:
interferon alpha 2
Target Gene Accession:
NM_000605.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Alpha interferon suppresses the cyclin D3 and cdc25A genes, leading to a reversible G0-like arrest.
- Angiogenic activity of rat mast cells in the chick embryo chorioallantoic membrane is down-regulated by treatment with recombinant human alpha-2a interferon and partly mediated by fibroblast growth factor-2. (alpha-2a interferon)
- Possession of the IFNA17 polymorphism (551T-->G), with higher levels of IFN-alpha significantly increases the risk of sarcoidosis.
- Data describe the mapping of the complete binding region of IFNAR1 on IFNalpha2 using a panel of 21 single alanine mutant proteins.
- In vivo induction of ITGA-11 is detected in spleen and lungs of human recombinant IFN-alpha 2-treated BALB/c mice.
- Findings indicate that the deletion in the promoter of the IFN-alpha-2 gene reduces the transcription of this gene in vitro and this could also be one cause of susceptibility to hepatitis B.
- Plasmacytoid dendritic cells in atherosclerotic plaque sense microbial motifs and amplify cytolytic T-cell functions. IFN-alpha emerged as a potent regulator of T-cell function.
- Differential transcription occurring early in atopic dermatitis skin was indicated for CCL18, CCL13, IFNalpha2, PPARalpha and PPARgamma.
- study reports that, in addition to promoting T cell chemotaxis, IFN-alpha2 enhances T cell adhesion to integrin ligands, which is associated with integrin clustering on the T cell surface and enhanced conjugate formation with dendritic cells
- IFNalpha is able to interfere with IL-6 signaling by inhibiting STAT3 activity and the abrogation of STAT3 activity accounts for the ability of IFNalpha to induce apoptosis in myeloma cells.
- we propose that HIV-1 expresses Vpu to counteract an IFNalpha-induced, general host defense that inhibits dissemination of enveloped virions from the surface of infected cells.
- Results demonstrated that two individual IFN-alphas interact differentially with IFNAR2-EC and influence each other during this interaction.
- Recombinant human IFN-alpha 2b causes programmed cell death in nondiseased renal epithelial cells. IFN-alpha-induced apoptosis is directed by an extrinsic death receptor signaling pathway, amplified by an intrinsic mitochondrial pathway.
- Recombinant INFA2 may have antitumor activity against Merkel cell carcinoma.
- IFN-alpha2b possibly controls chemotaxis by regulating the interaction between CXCL10 and CXCR3A
- Mutation of the IFNAR-1 receptor binding site of human IFN-alpha2 generates type I IFN competitive antagonists.
- The worldwide distribution of indel polymorphism in the gene coding for IFNA2 was analyzed.