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Validated All-in-One™ qPCR Primer for HSPA8(NM_006597.5) Search again
Product ID:
HQP009086
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
Gene Description:
heat shock protein family A (Hsp70) member 8
Target Gene Accession:
NM_006597.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein.
Gene References into function
- Tumor-derived HSP70 peptide complexes have the immunogenic potential to instruct dendritic cells to cross-present endogenously expressed, nonmutated, and tumor antigenic peptides shared among tumors of the melanocytic lineage for T cell recognition.
- Hsc70 may be involved in androgen action on dermal papilla cells.
- no abnormal levels of hsc70 and hsp60 were detectable in pediatric c-ALL pre B-cells at diagnosis nor at relapse. In contrast, developmentally regulated levels of hsc70 and hsp60 expression during B-cell ontogenesis were observed
- tau binds to Hsc70, and its phosphorylation is a recognition requirement for the addition of ubiquitin
- a novel nuclear export and nuclear localization-related signals in human Hsp70
- a region of the TfR that can potentially interact with hsc70
- sequestered in discrete foci in the nucleus of the infected cell in herpes simplex virus type 1 infection.
- Hsp105alpha is suggested to regulate the substrate binding cycle of Hsp70/Hsc70 by inhibiting the ATPase activity of Hsp70/Hsc70, thereby functioning as a negative regulator of the Hsp70/Hsc70 chaperone system.
- Expressed in colon cancer cells [NIP71]
- The head shock protein 70 kD (HSP-70i) has been shown to protect cells, tissues, and organs from harmful assaults in vivo and in vitro experimental models.
- In this study, we discovered that the U-box ubiquitin ligase carboxyl-terminus of Hsc70 interacting protein (CHIP) ubiquitylated Epsin.
- Present in cells as part of the dengue virus receptor.
- CSP modulates G protein function by preferentially targeting the inactive GDP-bound form of G alpha(s) and promoting GDP/GTP exchange; the guanine nucleotide exchange activity of full-length CSP is regulated by Hsc70-SGT
- This study identified heat shock protein 70 protein 8 as being expressed on the cell surface and downregulated upon differentiation.
- Thus, these findings suggest that Hsc70 associates with unliganded hERalpha, and thereby deters hERalpha from recruiting transcriptional coregulators, presumably as a component of chaperone complexes.
- Histidine 89 is an essential residue for Hsp70 in the phosphate transfer reaction.
- Hsc70 is directly associated with Influenza virus matrix protein 1 and therefore is required for viral production
- Our results suggest that the widespread accumulation of Hsc70 and Hsp70 may occur in brains with MSA, and that Hsc70 and Hsp70 may be associated with the pathogenesis of MSA.
- Hsc70 plays a defined role in reovirus outer capsid disassembly, during or soon after membrane penetration, to prepare the entering particle for gene expression and replication
- serum Hsp70 concentrations are decreased in normal human pregnancy
- Following co-immunoprecipitation, a specific interaction between the head domain of K5 with Hsc70, a chaperone also involved in vesicle uncoating, was verified.
- FKBP38 is a co-chaperone of HERG and contributes via the Hsc70/Hsp90 chaperone system to the trafficking of wild type and mutant HERG potassium channels
- 70-kDa heat shock proteins as a AQP2 interactors and have shown for hsc70 that this interaction is involved in AQP2 trafficking.
- Important functions of recently activated dendritic cells are thus critically modulated by the newly discovered HSPA8-EWI-2 interaction.
- Amino acid domains 280-297 of VP6 and 531-554 of VP4 are implicated in heat shock cognate protein hsc70-mediated rotavirus infection.
- an association between Hsc70 and ASIC2 that may underlie the increased retention of ASIC2 in the endoplasmic reticulum of glioma cells.
- HSP70 was a useful indicator of stressed neurons in acute phase of epilepsy, but not associated with neuronal death
- Our data show that a mutation in hsp70-Hom gene is associated with higher incidence of Postoperative atrial fibrillation.
- BAG-1 associates with Tau protein in an Hsc70-dependent manner.
- propose that one function of the Hsc70 nuclear foci may be to serve to facilitate the process of clearing stalled RNAP II complexes from viral genomes during times of highly active transcription
- Association between endogenous LAP2alpha and Hsp70 in non-transfected cells was confirmed by co-immunoprecipitation.
- Our results raise the possibility that HSP70 gene (i.e., haplotypes of rs2075799) might be implicated in the development of schizophrenia.
- Cathepsin C propeptide interacts with intestinal alkaline phosphatase (IAP) and heat shock cognate protein 70. The propeptide of cathepsin C may stimulate the sorting to the lysosome contributing to the degradation of IAP in Caco-2 cells.
- It was indicated that disruption of the HSC73-TAP association resulted in inhibition of TAP-dependent translocation of HSC73-bound peptides.
- During the earliest stages of HSV-1 infection, the formation of Hsc70 foci adjacent to viral prereplicative sites occurs after PML bodies have been disrupted by ICP0. PML protein is not required for the formation of Hsc70 foci.
- hsc70 binds tau at two sites that are involved in tau aggregation, one of which is regulated by alternative splicing; hsc70 binding might directly inhibit tau-tau interactions that precede tau oligomerization and aggregation.
- The variant allele of THBS2 is a risk factor for TAA in hypertensive patients, whereas the variant alleles of HSPA8, GPX1, AGT, and TNF are protective against this condition
- the hydrophobicity of the TA region dictates whether a precursor is delivered to the ER via the Hsp40/Hsc70 or Asna-1/TRC40-dependent route.
- Bag1 NEF increased refolding by Hsc70 and DJA2, as did the newly characterized NEF Hsp110
- Simultaneously reducing the expression of both HSC70 and HSP72 induces proteasome-dependent degradation of HSP90 client proteins, G1 cell-cycle arrest, and extensive tumor-specific apoptosis in human tumor cell lines.
- altered expression of proteins that underlie pathogenesis of SCA17
- This protein has been found differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.