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Validated All-in-One™ qPCR Primer for HOXB4(NM_024015.4) Search again
Product ID:
HQP008990
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HOX-2.6, HOX2, HOX2F
Gene Description:
homeobox B4
Target Gene Accession:
NM_024015.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17.
Gene References into function
- may play a role in the regulation of cellular proliferation/adhesion in developing fetal human epidermis and in hyperproliferation conditions, including cancers, in adult epidermis
- Retrovirally mediated expression of HOXB4 rapidly triggers an increase in the number of stem cells without alternating the balance of lymphomyeloid reconstitution, suggesting that HOXB4 does not affect control of end-cell output.
- In NOD/SCID mice HOXB4-overexpressing cord blood CD34+ cells had a selective growth advantage in vivo. However, high HOXB4 expression substantially impaired myeloerythroid differentiation & B-cell output.
- NF-Y is a developmentally regulated inducer of the HOXB4 gene in hematopoietic cells.
- Data report novel nucleoporin 98 fusions with homeobox (HOX)A10, HOXB3 and HOXB4, and describe the results of coexpression of these proteins with the Hox cofactor Meis1 in leukemic induction.
- The growth promoting effects of HOXB4 are critically dependent on HOXB4 expression levels and that this can result in important species-specific differences in potency.
- The protein transduction domain-Hoxb4protein can be used with other recombinant proteins to efficiently generate transplantable hemaat4opoietic stem cells from human embryonic stem cells.
- contribution of HOXB4 in the maintenance of the intrinsic lymphomyeloid differentiation potential of defined hematopoietic progenitor cell subsets
- These data suggest that HoxB4-induced effects on human embryonic stem cell-derived hematopoietic stem cells are concentration-dependent during in vitro development and reduce proliferation of other cell types in vitro and in vivo.