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Validated All-in-One™ qPCR Primer for TLX1(NM_005521.3) Search again
Product ID:
HQP008964
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HOX11, TCL3
Gene Description:
T cell leukemia homeobox 1
Target Gene Accession:
NM_005521.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- A PBX2 Regulatory Element PRE-1048 has been identified which contains a novel DNA-binding sequence and mediates significant activation of the HOX11 gene in K562 cells.
- Seven conserved Cdx binding sites were found in the Hoxa 11 promoter, suggesting regulatory sites.
- Hox11 expression is linked to chromosomes 5 and 14 in T-cell malignancies in children.
- HOX11 manifests its regulatory function via its action as a transcription factor
- transcriptional deregulation of G1/S growth-control genes, mediated in large part through blockade of PP1/PP2A phosphatase activity, plays an important role in HOX11 pathobiology
- HOX11 overexpression leads to a heightened predisposition for development of aneuploidy, contributing to oncogenesis
- TLX1/HOX11 overexpression and Ubr1 inactivation in promoting chromosome missegregation, permitting the accrual of additional chromosome losses and cytogenic abnormalities en route to malignancy.
- TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains
- TLX1(+) T-cell acute lymphoblastic leukemias be defined as cases expressing TLX1/ABL ratios greater than 1 and/or demonstrating TLX1 rearrangement
- Hox11 is expressed in spleen and may have a role in acute lymphocytic leukemia
- These results characterize TLX1 as a dual function regulator whose activity in respect to FHL1 is critically dependent upon its cellular concentration, as well as cell type and promoter context.
- LMO2, TAL1, Ttg-1, and SIL support levels of V(D)J recombination above background levels in cell culture and are also cleaved by the RAG proteins, while Hox11 and SCL are nicked but not cleaved efficiently in vitro
- Application of expression of HOX 11 allows the prospective evaluation of prognostic effects within pediatric and adult protocols of patients with T cell lymphoblastic lymphoma.
- HOX11/TLX1 stem cells are uniquely and abundantly expressed throughout adulthood in the human spleen.
- Aberrant HOX11 expression is associated with thymic adult T-cell acute lymphoblastic leukemia