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Validated All-in-One™ qPCR Primer for HNRNPK(NM_002140.4) Search again
Product ID:
HQP008955
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AUKS, CSBP, HNRPK, TUNP
Gene Description:
heterogeneous nuclear ribonucleoprotein K
Target Gene Accession:
NM_002140.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession.
Gene References into function
- Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
- c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs(hnRNPK)
- hnRNP K KH3 specifically recognizes a tetrad of sequence 5'd-TCCC. The complex is stabilized by a dense network of methyl-oxygen hydrogen bonds involving the methyl groups of 3 isoleucine residues and the O2 and N3 atoms of the 2 central cytosine bases.
- examination into mechanisms of hnRNP K activities by identifying protein factors that interact with it
- we describe the identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a protein that specifically interacts with Sam68 in vitro and in vivo.
- acts together with Pur(alpha) to repress the transcriptional activity of the CD43 gene promoter during lymphocyte activation
- Nuclear shift of hnRNP K protein in dividing cells may reflect its involvement in signalling multiple events that regulate expression of genes in proliferating cells
- Co-crystallization of the third KH domain of human hnRNP K with a 15-mer ssDNA showed that the crystals contained the complex containing three KH3 domains per 15-mer ssDNA
- ORF57 and hnRNP K interaction may modulate ORF57-mediated regulation of viral gene expression
- hnRNP K is a positive effector of collagen synthesis acting at the post-transcriptional level by interaction with the 3'-untranslated regions (3'-UTRs) of COL1A1, 1A2, and 3A1 mRNAs.
- the mutually antagonistic action of two RNA-binding proteins, Hu and hnRNP K, control the timing of the switch from proliferation to neuronal differentiation through the post-transcriptional regulation of p21 mRNA
- hnRNP K is involved in B cell receptor signalling pathway
- NMR and X-ray crystallographic studies of the third KH domain of hnRNP K in complex with single-stranded nucleic acids
- HNRPK down-regulation and interference with HNRPK translation-but not transcription-regulatory activity impairs proliferation, clonogenic potential, and leukemogenic activity of BCR/ABL-expressing myeloid 32Dcl3 and/or primary CD34+ CML-BC cells
- hnRNP K is a HDM2 target and serves as a cofactor for p53. HnRNP K plays key roles in coordinating transcriptional responses to DNA damage.
- Alternative isoform of hnRNPK found in colonic tumor and surrounding mucosa; is first example of RNA editing even in cancer and its surrounding tissue
- arginine dimethylation of heterogeneous nuclear ribonucleoprotein K by protein-arginine methyltransferase 1 inhibits its interaction with c-Src
- satellite I RNA binds to hnRNP K protein
- These findings provide a putative mechanism by which transcriptional activity of hnRNP K can be discretely controlled through the regulation of PP1 activity.
- Tandem mass-spectrometric analysis of the peptide at residues 288-303 of heterogeneous nuclear ribonucleoprotein K (hnRNP K) shows that both Arg296 and Arg299 are dimethylated.
- Isolation of antibodies from cells with loss of migration phenotype and identification of their target proteins revealed the involvement of the heterogeneous nuclear ribonucleoprotein K (hnRNP-K), a multifunctional signaling protein, in metastasis
- interacts with Sindbis virus NSP2 and viral subgenomic mRNA
- Heterogeneous nuclear ribonucleoprotein K overexpression in oral squamous cell carcinoma.
- APOBEC3 suppresses HBV replication in hepatocytes by inhibiting hnRNP K-mediated transcription and expression of HBV genes as well as HBV core DNA synthesis.
- hnRNP K binds to the 3'-untranslated region of the c-Src mRNA and inhibits its translation by blocking 80 S ribosome formation
- Inhibition of methylation in hnRNP K attenuated the recruitment of p53 to p21 promoter, and reduced p53 transcriptional activity.
- Cytoplasmic hnRNP K and high thymidine phosphorylase may be potential prognostic and therapeutic markers for nasopharyngeal carcinoma.
- The interaction of hnRNP-K with Nef strongly increased HIV transcription, which depended on Tat and the NF-kB motif in the viral promoter, but not on NF-kB activation.
- HNRNP K and microRNA-16 have roles in cyclooxygenase-2 RNA stability induced by S100b, a ligand of the receptor for advanced glycation end products
- Regulation of the hTERT promoter activity by MSH2, the hnRNPs K and D, and GRHL2 in human oral squamous cell carcinoma cells.
- The data here provide possible mechanisms of how the non-enzymatic activity of PRMT family protein associates with hnRNP K protein and regulates hnRNP K protein-involved transactivation functions.
- Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
- hnRNP K and RBM42 have a role in the maintenance of cellular ATP level in the stress conditions possibly through protecting their target mRNAs.
- MDM2 released from p53 by RITA promotes degradation of p21 and the p53 cofactor hnRNP K, required for p21 transcription