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Validated All-in-One™ qPCR Primer for ANXA2(NM_001002858.2) Search again
Product ID:
HQP008717
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV
Gene Description:
annexin A2
Target Gene Accession:
NM_001002858.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
Gene References into function
- identification of a hetereotetramer as a plasmin reductase
- amino acids critical for tissue-type plasminogen activator-annexin A2 interaction
- results suggest that annexin II, and, likely, annexin I, may be endogenous suppressors of prostate cancer cell migration and their reduced or lost expression may contribute to prostate cancer development and progression
- virtual Northern blots to analyze expression of NACA and ANX2 in progenitor cultures of nine children with Juvenile myelomonocytic leukemia and five healthy individuals
- analysis of annexin A2 interaction with tissue plasminogen activator, plasminogen, and plasmin
- annexin 2/S100A10 complex functions in the intracellular positioning of recycling endosomes and that both subunits are required for this activity
- annexin II-mediated assembly of plasminogen and t-PA on monocyte/macrophages contributes to plasmin generation, matrix remodeling, and directed migration
- annexin II binds to CEACAM1 in breast neoplasms
- possible mechanistic and regulatory role of Anx-II in prostate cancer progression
- ANXA2 is a Ca(2+)-dependent RNA-binding protein that interacts with the mRNA of the nuclear oncogene, c-myc
- AII does not bind 1,25-D(3) in a physiologically relevant manner
- Western blotting of endothelial cell surface of a number of sites showed higher amounts of tissue plasminogen activator annexin II on cerebral endothelial cell. In contrast, expression of u-PA receptor was the same for all ECs
- Lipocortin II may be a marker or contribute to asthma exacerbation.
- Annexin I serves as both ligand and receptor in promoting phagocytosis.
- E2A-HLF induces annexin II by substituting for cytokines that activate downstream pathways of Ras
- Reduced Annexin 2 expression is associated with osteosarcoma metastases
- Experiments using individual subunits identify annexin 2 as a PtdIns(4,5)P(2)-binding entity
- siRNA-mediated downregulation of annexin A2/S100A10 and disruption of the complex by microinjection of peptide competitors result in a marked reduction in von Willebrand Factor
- Temperature stress-induced annexin 2 translocation is dependent on both expression of protein p11 tyrosine phosphorylation of annexin 2.
- observations suggest a novel pathway for endothelial activation induced by APLA/anti-beta2GPI antibodies that is initiated by cross-linking or clustering of annexin A2 on the endothelial surface
- Human colon adenocarcinoma cell differentiation is associated with an up-regulation of AnxA1, AnxA2, and AnxA5 and with a subcellular relocation of these proteins
- investigation undertaken to identify the significance of annexin II expression and regulation in leukaemia do not fully support the concept of the coagulopathy associated with APL being caused by hyperfibrinolysis alone
- S100A10 and annexin A2 play an important role in plasmin regulation and in cancer cell invasiveness and metastasis [review]
- several single nucleotide polymorphisms in bone morphogenic protein 6, annexin A2, and klotho were associated with sickle cell osteonecrosis
- S100A4 protein alone or in a complex with annexin II accelerated tissue plasminogen activator-mediated plasminogen activation in solution and on the endothelial cell surface
- complex with S100A10 is a substrate of thioredoxin
- AX-II, in addition to inducing GM-CSF expression, increases membrane-bound RANKL synthesis by marrow stromal cells through MAPK-dependent pathway.
- Results show that progression from prostatic intraepithelial neoplasia to prostate cancer is characterized by a reduction of ANX2 and suggest that downregulation of these proteins could represent an important event in prostate carcinogenesis.
- annexin A1 and A2 may have roles in dysferlin deficiency and in muscular dystrophies
- Annexin A2 is part of the putative cell surface vitamin D binding protein binding site complex and functions to mediate the chemotactic cofactor effect.
- analysis of the structural determinant of the PtdIns(4,5)P2 selectivity of A2t (annexin II-p11 heterotetramer), which may be involved in the regulation of PtdIns(4,5)P2 clustering in the cell
- The experimental data suggest that the ANNEXIN A2 gene may relate to cellular apoptosis induced by p53 gene.
- anti-A2 antibodies contribute to the prothrombotic diathesis in antiphospholipid syndrome
- novel gag-binding protein essential for the proper assembly of HIV in monocyte-derived macrophages.
- annexin 2 mediated fibrinolysis on the transitional cell carcinoma cells may play a role in inducing hemorrhagic disorder in vascular intimal carcinomatosis
- Annexin II-dependent localized plasmin generation is associated with angiogenesis and metastasis in breast cancer
- Review discusses a role for annexin 2 in the regulation of ecto-5'-nucleotidase activity, which is dependent upon the stability of the membrane rafts.
- Real-time RT-PCR confirmed up-regulation of IL-8, osteopontin, and TNFRSF14 and down-regulation of SAMeS and CD209 in AH.
- analysis of calcium-dependent heparin binding to annexin A2
- Results describe the design, production, biophysical characterization and binding properties of a soluble mutant form of annexin A2 domain IV that adopts a partially folded conformation.
- that Annexin2 is required for strong binding of S100A10 to the C-terminal domain of the protein Ahnak.
- we further showed that LMP1 increased the serine, but not tyrosine, phosphorylation of annexin A2 by activating a novel signaling pathway, the protein kinase C (PKC) signaling pathway.
- The median of anti-annexin A2 IgM titer from preterm infants who were delivered with high-grade chorioamnionitis (CAM) was significantly higher than those from preterm infants without CAM and with low-grade CAM.
- demonstration of an angiostatin receptor (ANX2) in vascular endothelial tumors including angiosarcoma; diffuse and strong reactivity signified the absence of any down-regulation of ANX2 in both benign and malignant tumors
- following injury, N-glycosylation events and AII presentation on the cell surface of airway epithelial cells are important mediators in repair
- The anx 2-S100A10/CFTR complex is important for CFTR function across epithelia.
- uPA plays a major role in inducing MMP-1 production in activated human monocytes, which is mediated by a plasmin-dependent mechanism involving signaling through the annexin A2 heterotetramer.
- analysis of the pH-induced membrane binding of annexins A6 and A2-S100A10
- p11/S100A10 light chain of annexin A2 is dispensable for annexin A2 association to endosomes and functions in endosomal transport
- the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS.
- These results suggested that Annexin A2 may play roles in p53 induced apoptosis and it is also involved in regulation of cell proliferation.
- IRF-1 silencing with siRNA produced upmodulation of the Annexin-II protein in THP-1 cells.
- analysis of translocation and assembly at the plasma membrane; heterogeneous assembly formation was shown between annexins A5 and A2
- it is proposed that annexin 2 acts as a receptor for factor Xa on the surface of human umbilical vein endothelial cells and that annexin 2 facilitates factor Xa activation of PAR-1 but does not enhance coagulant function of factor Xa
- The findings suggest annexin 2 externalization is coupled to lipid efflux in prostate epithelium and that IFNgamma induces down-regulation of the protease-binding anx2(t) scaffold at the cell surface.
- 39.486N, 77.7W
- The effect of alcohol on fibrinolysis and plasmin is mediated in part by ANXA2. Alcohol directly influences hepatic pathways of fibrinolysis that may contribute to ALD.
- Rab14 also co-localized in part with annexin A2 and lamellar bodies in alveolar type II cells
- Annexin II and beta(2)-tubulin down-regulation is important in nasopharyngeal carcinoma formation and may represent potential targets for further investigations.
- annexin-2 is required for cAMP-induced AQP2 exocytosis in renal cells
- Within the endothelial cell, p11 is required for Src kinase-mediated tyrosine phosphorylation of A2, which signals translocation of both proteins to the cell surface.
- annexin II is a novel HSP27-interacted protein which is involved in UVC resistance in human cells
- Expression of ANAX2 in human atheroslerotic abdominal aortic aneurysms is reported.
- a tyrosine phosphorylation switch in annexin A2 is an important event in triggering Rho/ROCK-dependent and actin-mediated changes in cell morphology associated with the control of cell adhesion
- annexin II and its receptor axis play a central role in prostate cancer metastasis, and prostate cancer utilizes the hematopoietic stem cell homing mechanisms to gain access to the niche.
- Annexin II overexpression may induce gemcitabine resistance in pancreatic cancer resulting in rapid recurrence.
- identification of the minimal inhibitory sequence of AnxA2 should pave the way toward the development of PCSK9 inhibitory lead molecules for the treatment of hypercholesterolemia
- Annexin A2 has a role in malignant phenotype and secretion of IL-6 in DU145 prostate cancer cells
- phosphorylation of Tyr-23 is essential for proper endosomal association and function of AnxA2, perhaps because it stabilizes membrane-associated protein via a conformational change.
- EspL2 of E. coli O157:H7 Sakai bound F-actin-aggregating annexin 2 directly, increasing its activity.[EspL2]
- Annexin A2 expression and phosphorylation are up-regulated in hepatocellular carcinoma.
- Via its receptor annexin II on endothelial surface, tPA regulates intravascular fibrin deposition. Review.