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Validated All-in-One™ qPCR Primer for ANXA1(NM_000700.2) Search again
Product ID:
HQP008707
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANX1, LPC1
Gene Description:
annexin A1
Target Gene Accession:
NM_000700.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq].
Gene References into function
- ANXA1-derived peptides inhibit antigen-driven cellular proliferation and cytokine production in vitro.
- 17beta-estradiol promotes the synthesis and the secretion of annexin I in the CCRF-CEM human cell line.
- interferes with a leukocyte endothelial step essential for U937 cell, and possibly monocyte, transmigration both in vitro and in vivo
- ANXA1 secretion in lymphocytes is unlikely to involve the classical ER-Golgi pathway; requires a Ca(2+)-dependent cleavage of ANXA1; involves both Ca(2+)-dependent and independent mechanisms; and is apparently independent of the GC receptor alpha isoform
- This protein is translocated from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma.
- These results showed that annexin I binds to a surface receptor in order to regulate the stimulation of insulin release in MIN6N8a cells.
- The gene expression profile of ALD is dominated by alcohol metabolism and inflammation and differs from other liver diseases. Annexins may play a role in the progression of fibrosis in ALD.
- Annexin-I has a role in drug resistance in tumor cells
- annexin II serves as both ligand and receptor in promoting phagocytosis.
- ANXA1 has roles in neutrophil apoptosis [review]
- Annexin I was expressed in vivo in lungs containing metastasized MDA-MB-435 cells but not in normal lung tissue of athymic mice, suggesting that it is an important cellular protein that is down regulated through BRMS1 mediated metastasis suppression.
- an annexin 1 peptide can activate FPR, FPRL1, and FPRL2; results indicate that annexin 1 participates in regulating leukocyte emigration into inflamed tissue by activating and desensitizing different receptors of the FPR family.
- annexin I is not the tumor suppressor gene corresponding to the high levels of loss of heterozygosity observed on chromosome 9 in esophageal squamous cell carcinoma
- Data show that there is no association of these SNPs in ANXA1 gene with type 2 diabetes in Shanghai Han population.
- Human colon adenocarcinoma cell differentiation is associated with an up-regulation of AnxA1, AnxA2, and AnxA5 and with a subcellular relocation of these proteins
- annexin1 exerted its erythropoiesis regulating effect by ERK pathway
- Results show that progression from prostatic intraepithelial neoplasia to prostate cancer is characterized by a reduction of ANX1 and suggest that downregulation of this protein could represent an important event in prostate carcinogenesis.
- These results suggest that annexin A1 may be a key protein involved in CF pathogenesis especially in relation to the not well defined field of inflammation in CF.
- annexin A1 and A2 may have roles in dysferlin deficiency and in muscular dystrophies
- ANXA1 expression is decreased in prostatic adenocarcinoma and in high-grade prostatic intraepithelial neoplasia.
- gene expression of CCR10 was increased by recombinant ANXA1 and the N-terminal ANXA1 peptide
- ANXA1 and Gal-3 changed in their content and localization when neutrophils adhere to endothelia. In contrast, a decrease in the total amounts of Gal-1 was detected in migrated compared to non-migrated neutrophils.
- ANXA1 expression may correlate with the tumorigenesis suggesting that the protein may represent an effective differentiation marker in thyroid cancer.
- AnxA1 has a role in stimulating SKCO-15 cell migration through nFPR activation
- Annexin I may have tumor suppressor functions in prostate cancer. The pro-apoptotic effect of ANX I involves the activation of p38 and JNK, which appears to shift the balance of signal transduction away from proliferation and toward apoptosis.
- glucocorticoids and IL-1beta upregulate the synthesis and translocation of annexin I in rheumatoid synovial cells, but interdependent signalling pathways are involved
- The high ANXA1 expression is frequent in esophageal and esophagogastric junction adenocarcinomas, correlates with more advanced pathologic T stage and the presence of distant metastasis, and is an independent prognostic factor for patient survival.
- results suggest that suppressed ANXA1 expression in breast tissue is correlated with breast cancer development and progression
- In view of the wide range of endogenous ligands known to interact with FPRL-1, including the anti-inflammatory protein annexin-A1, we speculate that the novel effect here described may impact on the immunosuppressive properties of glucocorticoids.
- Ca2+ entering through a disruption locally induces annexin A1 binding to membranes, initiating emergency fusion events whenever and wherever required.
- CD4+ cells from patients with rheumatoid arthritis showed a marked up-regulation of annexin-1 expression; annexin-1 is a molecular "tuner" of TCR signaling
- Decrease of annexin-I expression is associated with bladder cancer development
- The identification of annexin 1 as a substrate for HLE supports the model in which annexin 1 participates in regulating leukocyte emigration into inflamed tissue.
- Annexin 1 has roles in a variety of inflammatory pathways, on cell proliferation machinery, in the regulation of cell death signaling, in phagocytic clearance of apoptosing cells, and most importantly in the process of carcinogenesis [review]
- the role of the homeostatic antiinflammatory axis centered on annexin 1 in cerebral microvascular dysfunction and tissue injury associated with middle cerebral artery occlusion and reperfusion
- Results demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth.
- Annexin-1 and peptide derivatives play a role in promoting the resolution of inflammation.
- proteinase 3 has a critical role in annexin 1 cleavage in activated neutrophils
- the confluence-dependent interaction of cPLA(2)alpha and annexin A1 at the Golgi acts as a novel molecular switch controlling cPLA(2)alpha activity and endothelial cell prostaglandin generation.
- Ca(2+)-dependent binding of S100A11 to ANXA1 facilitated the binding of the latter to cPLA(2), resulting in inhibition of cPLA(2) activity
- Cell surface ANXA1 synthesis is capable of blocking beta 2-integrin adhesion in PMNs. Fluticasone propionate does not cause ANXA1 synthesis or nuclear transport of cytosolic gIVaPLA2 & thus does not block beta2-integrin adhesion.
- Human clinical specimens showed that ANNA1 expression was reduced in bladder transitional cell carcinomas(TCC) compared to normal urothelium.
- analysis of translocation and assembly at the plasma membrane and the nuclear envelope
- Nuclear localization of annexin A1 is associated with oral squamous cell carcinoma
- N-terminal portion of annexin 1 plays some role in the pathogenesis of acute exacerbation in idiopathic pulmonary fibrosis patients
- PMN-derived microparticles contain functionally active AnxA1 that confers them anti-inflammatory properties
- Results describe decreased expression of annexin A1 during the progression of cervical neoplasia.
- demonstrated a novel mechanism of post-transcriptional regulation of ANXA1 expression and identified miR-196a as a marker of esophageal cancer
- Annexin-1 is overexpressed in eutopic endometrium and presents in the peritoneal fluids of patients with endometriosis, and may play a role in the pathogenesis of endometriosis.
- Report the occurrence of autoantibodies to annexin I, 14-3-3 theta and LAMR1 in prediagnostic lung cancer sera.
- Annexin A1 dysregulation was observed early in laryngeal carcinogenesis, in intra-epithelial neoplasms; it was not found related to prognostic parameters, such as nodal metastases
- glucocorticoids regulate the expression and function of annexin A1 in opposing ways in innate and adaptive immune cells to mediate the resolution of inflammation. Review.
- Loss of Annexin-1 is associated with invasive breast cancer.