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Validated All-in-One™ qPCR Primer for GZMK(NM_002104.2) Search again
Product ID:
HQP008691
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
TRYP2
Gene Description:
granzyme K
Target Gene Accession:
NM_002104.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes.
Gene References into function
- The 2.2-A crystal structure reveals a rigid zymogen with unusual features
- The present findings thus introduce the possibility that human beta-tryptase, after mast cell degranulation and exposure to neutral pH in the tissue, may dissociate into active monomers with properties that are distinct from the tetrameric counterpart.
- We found human GzmK triggers rapid cell death independently of caspase activation. The features of death are characterized by rapid externalization of phosphatidylserine, nuclear morphological changes and single-stranded DNA nicks.
- GzmK-induced caspase-independent death occurs through Bid-dependent mitochondrial damage that is different from GzmA
- Rsults indicate that plasma levels of Granzyme K could serve as a useful diagnostic marker to stage sepsis, permitting better classification, specific treatments of patients, and may play a functional role in the development of sepsis.
- p53 is as a cytotoxic bomb that can be triggered by granzyme K, leading to potentiating killing efficacy.