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Validated All-in-One™ qPCR Primer for TBX21(NM_013351.1) Search again
Product ID:
HQP008682
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IMD88, T-PET, T-bet, TBET, TBLYM
Gene Description:
T-box transcription factor 21
Target Gene Accession:
NM_013351.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box.
Gene References into function
- distribution of T-BET/TBX21 expression in the haematopoietic compartment
- Among twenty-three novel polymorphisms identified in the human TBX21 gene of Korean asthma patients, no significant associations of TBX21 variants with risk of asthma were observed.
- VZV virus and mite antigens induce expression of this transcriptionn factor in cord blood mononuclear cells, differenetially affecting Th1 and Th2 cells.
- T-bet expression in T(H)2 cells induced IL-2 production and decreased the secretion of IL-4.
- Association seen between type 1 diabetes and polymorphisms in the T-bet gene; variation in T-bet transcriptional activity may play a role in the development of type 1 diabetes, possibly through the effect on IFN-gamma production in Th1 cells
- genetic variation in TBX21 may alter asthma phenotypes in a treatment-specific fashion
- Expressed in Hodgkin disesase Reed Sternberg cells.
- Data indicate that T-bet can override repressive epigenetic modification through a T-box half-site and dissociation of the mSin3a corepressor from the promoter.
- T-bet may play a role in Hodgkin's lymphoma oncogenesis
- first report demonstrating relationship between TBX21 single nucleotide polymorphisms and aspirin-induced asthma in Japanese
- female steroid hormones use Stat-mediated pathways to modulate the expression of T-bet in epithelial cells
- T-bet was clearly induced in the two B-cell precursor-leukemia cell lines.
- These data suggest that T-bet variation contributes to airway responsiveness in asthma.
- Data from a prospective twin study suggest that T helper type 2 (Th2) cell-associated diseases in humans might be due to genetic variations in Th1 cytokine regulation via T-bet.
- A key transcription factor that links the long-term renewal of memory CD8+ T cells to their characteristic effector potency.
- Increased IFN-gamma levels observed in aplastic anemia patients are the result of active transcription of the IFN-gamma gene by T-bet.
- Increased T-bet expression does not only identify intracellular infections in lymphoid tissue associated with high IFN-gamma levels, but also implies that, under these conditions, it becomes induced in B cells, which apparently support the Th1 response.
- Segmental allergen challenge in asthmatics leads to increased GATA-3, c-maf and T-bet expression in BAL cells but not in bronchial biopsies
- the GATA-3/T-bet transcription factor complex regulates the cell-lineage-specific expression of the lymphocyte homing receptors
- A defect in Wiskott Aldrich CD4+ T cells is due to reduction in T-bet mRNA expression and in early nuclear recruitment of NFAT-1.
- There is an association between expression of Th1/Th2 transcription factors and cytokines (T-bet, GATA-3, IFN-gamma, IL-4,IL-18) in systemic lupus erythematosus.
- suppression of T-bet ameliorates EAE by limiting the differentiation of autoreactive Th1 cells, as well as inhibiting pathogenic Th17 cells via regulation of IL-23R.
- T-bet gene might be involved in the development of NK/T-cell lymphoma.
- Genetic variations the TBX21 promoter region contribute to susceptibility to HBV infection in the Chinese population.
- IL-4 gene transcription is inhibited by T-bet via the suppression of its promoter activity, independently of IFN-gamma. T-bet facilitates Th1 differentiation through not only upregulation of IFN-gamma, but also downregulation of IL-4 gene transcription.
- Immunohistochemical detection of T-bet in infiltrated bone marrow trephines represents an important adjunct for the diagnosis of hairy cell leukemia.
- Sp1 is a positive transcriptional regulator of T-BET
- An association between a specific TBX21 haplotype and allergic asthma in children is demonstrated for the first time.
- The expression patterns of T-Bet and GATA-3 oppose progesteron receptor, suggesting antagonistic function and/or regulation between PR and T-Bet/GATA-3
- low-dose prolactin induced T-bet expression and high-dose prolactin tended to suppress it
- IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2-related diseases.
- evaluated the frequencies of five different single-nucleotide polymorphisms (SNPs) in the TBX21 gene in 159 HSV-2-infected individuals and compared them with those in 186 healthy HSV-2-seronegative controls