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Validated All-in-One™ qPCR Primer for A2M(NM_000014.5) Search again
Product ID:
HQP008678
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
A2MD, CPAMD5, FWP007, S863-7
Gene Description:
alpha-2-macroglobulin
Target Gene Accession:
NM_000014.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq].
Gene References into function
- Differential binding to ldl receptor related protein
- distinct binding sites mediate interaction with beta-amyloid peptide and growth factors
- has an important role in the AD-specific neurodegenerative process but its exon 24 Val-1000-Ile polymorphism is not likely to be associated with late-onset sporadic AD in the Hungarian population
- REVIEW: binds and neutralizes alfimeprase, which has direct proteolytic activity against the fibrinogen Aalpha chain
- The three-dimensional structure of the dimer reveals its structural organization in the tetrameric native and chymotrypsin alpha 2-macroglobulin complexes.
- relationship between serum VEGF levels, alpha(2)M levels and the development of OHSS in hyperstimulated subjects undergoing IVF
- Alpha 2-macroglobulin enhances prothrombin activation and thrombin potential by inhibiting the anticoagulant protein C/protein S system in cord and adult plasma.
- Genetic association of argyrophilic grain disease with polymorphisms in alpha-2 macroglobulin.
- Genetic association of alpha2-macroglobulin polymorphisms with Alzheimer's disease
- These results suggest the possible involvement of cathepsin E in disruption of the structural and functional integrity of alpha 2-macroglobulin in the endolysosome system.
- alpha(2)M-derived peptides target the receptor-binding sequence in TGF-beta
- FGF-2 and this protein interact at specific binding sites, involving different FGF-2 sequences.
- There is a significant genetic association of the 5 bp deletion and two novel polymorphisms in alpha-2-macroglobulin alpha-2-macroglobulin precursor with AD
- alpha2-macroglobulin inhibits human pepsin and gastricsin
- alpha2-M deletion polymorphism is probably not associated with functional deficiencies important in Alzheimer's disease pathology
- A2M-D allele played a weak Alzheimer disease protective role, and APOE-E4 and A2M-G alleles might act synergistically in Alzheimer disease risk for mainland Han Chinese.
- Plasma from patients homozygous for the intronic deletion (DD) showed normal alpha(2)M subunit size, conformation, and proteinase inhibitory activity. Plasma alpha(2)M from two DD patients showed markedly increased TGF-beta1 binding.
- Alpha2-macroglobulin is a substrate and an endogenous inhibitor for ADAMTS-4 and ADAMTS-5
- The presence of MPO-G/G and A2M-Val/Val genotypes synergistically increased the risk of AD (OR, 25.5; 95% CI, 4.65-139.75).
- an important involvement of alpha2M in regulation of increased proteolytic activity occurring in multiple sclerosis disease
- increased alpha(2)-macroglobulin expression appears to be associated with benign prostatic hyperplasia
- PAK-2 is activated in 1-LN prostate cancer cells by a proteinase inhibitor, alpha 2-macroglobulin
- These studies demonstrate that a single mutation in full-length r(alpha)2M is sufficient to block binding to LRP-1.
- A2M gene was suggested to be associated with Alzheimer's disease.
- results indicate that secondary proteolysis of alpha2-macroglobulin promotes impaired control of extracellular proteolytic activity, leading to local and distant tissue injuries during severe acute pancreatitis
- review of the A2M gene families of mice and humans, the predicted structural relationships between A2M and its pregnancy induced forms and the postulated roles for this gene family in normal pregnancy
- the inhibition of proteases on the surface of microorganisms by an ancestral alpha2M-like thiol ester protein may generate "arrays" of oligomannose glycans to which MBL or other lectins can bind
- PDGF-BB and TGF-beta1 binding to intact alpha2M is specific, involving a defined region of the alpha2M subunit
- In this proteins two amino acid polymorphisms (Ile/Val A-->G) have been associated with an increased risk for Alzheimer's disease (AD) and the combination with CTSD-T allele seems to increase this risk.
- results did not support the hypothesis that A2M deletion polymorphism is associated with a low A2M concentration in overweight/obese subjects
- bone morphogenetic protein 1 is inhibited by native and altered forms of alpha2-macroglobulin
- alpha(2)M* binding to LRP-1 induces cell proliferation and MAPK activation in the J774 macrophage-derived cell line
- The risk of symptomatic thromboembolism was significantly increased with elevated alpha2MG levels.
- alpha2-macroglobulin may facilitate conformational changes in prion protein in spontaneous forms of prion disease
- expression and characterization of three alpha(2)M domains predicted to be involved in the stabilization of the thiol ester in native alpha2M and in its activation upon bait region proteolysis.
- Alpha 2M activated by neutrophil elastase to its receptor-recognized form binds CpG ODN and enhances their immunostimulatory properties significantly.
- Decreased expression of alpha2-M mRNA and protein and protease inhibitory activity in the vaginal wall tissues of women with stress urinary incontinence(SUI) may contribute to the development of SUI.
- alpha2M is a newly discovered and unique member of a small group of abundant extracellular proteins with chaperone properties
- Senile systemic amyloidosis was associated with age, myocardial infarctions, the G/G (Val/Val) genotype of the exon 24 polymorphism in the alpha2-macroglobulin (alpha2M), and the H2 haplotype of the tau gene
- effects of intact alpha(2)M on cell physiology reflect the degree of penetration of activities associated with different domains
- Copper is taken up efficiently from albumin and alpha2-macroglobulin by cultured human cells by more than one mechanism.