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Validated All-in-One™ qPCR Primer for GSN(NM_000177.4) Search again
Product ID:
HQP008470
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ADF, AGEL
Gene Description:
gelsolin
Target Gene Accession:
NM_000177.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- crystal structure: loss of a metal-binding site leads to familial amyloidosis-Finnish type
- functions as a regulator of cell growth and apoptosis
- ATF-1 DNA-binding activity was abundant in breast cancer cells and correlated inversely with gelsolin mRNA levels. This suggests a role for ATF-1 in gelsolin promoter silencing, in contrast to its transactivating effect on various other promoters.
- explores the structure-function relationship of the gelsolin-PPI interaction
- Calcium activation of gelsolin: X-Ray crystallography model of the G4-G6/actin complex.
- Gelsolin suppresses tumorigenicity through inhibiting PKC activation in a human lung cancer cell line, PC10.
- Its reduced expression in bladder cancer is regulated by DNA methylation, histone acetylation and chromatin remodeling.
- A visualization of the Ca2+-dependent activation of this protein by using synchrotron footprinting.
- analysis of domain-binding and calcium-binding sites in gelsolin
- Gelsolin and plasminogen activator inhibitor-1 have roles as Ap3A-binding proteins
- conformational dynamics of ligand-induced structural changes
- structure of the isolated C-terminal half of gelsolin (G4-G6) at 2.0 A resolution in the presence of Ca(2+) ions
- 2.6 A resolution crystal structure of a complex formed between G-actin and gelsolin fragment Met25-Gln160
- Data suggest that mutations that diminish domain 2 Ca(2+) binding allow furin access to an otherwise protected cleavage site, initiating the proteolytic cascade that leads to gelsolin amyloidogenesis and familial amyloidosis of Finnish type.
- the observed ubiquitous increase of gelsolin in the senescent states of cells and tissues, and the increased sensitivity to apoptosis-induction by gelsolin down-regulation, suggests that it would be partly responsible for age-related apoptosis resistance
- Gelsolin is involved in the apoptotic resistance in senescent human diploid fibroblasts.
- gelsolin protects cells from butyrate-induced apoptosis
- The structure of the N-terminal half of gelsolin bound to actin was studied, as well as its roles in severing, apoptosis, and familial amyloidosis.
- IFNalpha can induce morphological cell changes that are peculiar of apoptosis onset through the caspase-3-mediated cleavage of gelsolin.
- Analysis of major platelet membrane proteins revealed significant increase in gelsolin from patients with homozygous and heterozygous forms of beta-thalassemia.
- model of the N-terminal extension on the surface of the gelsolin molecule, which was unknown previously
- Gelsolin has a role as a regulator of cellular functions [review]
- Gelsolin is partly buried in the lipid bilayer at low pH. The linker regionbetween G3 and G4 is involved in this interaction. Vesicle-bound gelsolin can bind to actin filaments, presumably through barbed end capping.
- features of gelsolin amyloidogenic fragments comprised of residues 173-243 and residues 173-202 were investigated
- Mechanisms of GSN dependent and independent EGF stimulated cell motility were studied in an epithelial cell line.
- The tight interaction between plasma gelsolin and endotoxin/lipopolysaccharide (LPS) affects both gelsolin's actin binding function and some aspects of the effects of LPS on cells.
- gelsolin functions as a switch that controls E- and N-cadherin conversion via Snail, and demonstrated that its knockdown leads to epithelial-mesenchymal transition in human mammary epithelial cells and possibly to the development of human mammary tumors
- Heparin efficiently accelerates the formation of gelsolin amyloid by enabling intermolecular beta-sheet formation. In addition, heparin accelerated aggregation at both early and late stages of amyloidogenesis
- posttranslational N-myristoylation of tGelsolin does not direct mitochondrial targeting, but this modification is involved in the anti-apoptotic activity of tGelsolin
- higher expression in MCM2 and gelsolin was significantly associated with poorer prognosis in patients with NSCLC, which suggests that higher tumor proliferation and motility may be important in the prognosis of NSCLC
- A functional, cooperative relationship between endogenous N-RAS and gelsolin is identified in colorectal cancer cell lines that correlates with survival.
- These data indicate that actin cytoskeletal dynamics modulate the tyrosine phosphorylation of raft-associated proteins and subsequent downstream signal transduction.
- gelsolin G5 domain inhibits HIV-Vpr-induced T-cell apoptosis by blocking the interaction between Vpr and VDAC
- Calcium induced conformational changes in the G1-2 and G1-3 sub-domains of gelsolin, and the binding affinities for the three type II sites, are reported.
- A new family with Meretoja syndrome is reported. This is the first documented family with Meretoja syndrome in Spain and in the Mediterranean countries. The molecular study shows the same mutation of reported families.
- analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity
- Ardalan-Shoja-Kiuru syndrome, a hereditary gelsolin amyloidosis plus retinitis pigmentosa, is due to a G654A gelsolin mutation.
- TP53 mutations are frequent events in bladder cancer progression and gelsolin relates to TP53 status, tumor staging and clinical outcome.
- These results suggest that Egr-1 may be an important breast cancer marker and that an as yet uncharacterized pathway involved in Egr-1 and gelsolin expression exists which leads to breast cancer cell development.
- Gelsolin levels are actively downregulated in pancreatic cancer and enhanced targeting of gelsolin to the ubiquitin-proteasome pathway is an important contributing factor for this effect.
- Gelsolin down-regulation is an early and almost constant event in colon carcinogenesis and is associated with the transition from adenoma to carcinoma.
- gelsolin is an anti-amyloidogenic agent that can reduce amyloid load by acting as an inhibitor of Abeta fibrillization, and as an antioxidant and anti-apoptotic protein
- Interaction of gelsolin with lipopolysaccharide and lipoteichoic acid results in both inhibition of gelsolin's actin-severing activity and the ability of the bacterial toxins to induce an immune response in vitro.
- The plasma isoform of gelsolin is decreased in the plasma of patients with rheumatoid arthritis
- Down-regulation of gelsolin by siRNA transfection reveals its involvement in promoting and inhibiting pancreatic cancer cell lines.
- plasma gelsolin deficiency are associated with early sepsis.