Validated All-in-One™ qPCR Primer for GRM7(NM_000844.3) Search again

Buy	Catalog#	Description	Note	Price (US$)
	HQP008452	All-in-One™ qPCR Primer for NM_000844.3 (200 reactions)	GRM7	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.6 (200 reactions)	Reference Gene GAPDH	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.7 (200 reactions)	Reference Gene GAPDH	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.3 (200 reactions)	Reference Gene B2M	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.4 (200 reactions)	Reference Gene B2M	inquire
	HQP016381	All-in-One™ qPCR Primer for NM_001101.5 (200 reactions)	Reference Gene ACTB	inquire

Validated result:

A	B
Figure: All-in-One™ qPCR Primer Validated result to GRM7. Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene References into function