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Validated All-in-One™ qPCR Primer for ANPEP(NM_001150.2) Search again
Product ID:
HQP008414
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AP-M, AP-N, APN, CD13, GP150, LAP1, P150, PEPN, hAPN
Gene Description:
alanyl aminopeptidase, membrane
Target Gene Accession:
NM_001150.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections.
Gene References into function
- data demonstrate that the glycosylation of aminopeptidase N at N291 blocks human coronavirus-229E infection
- tested the hypothesis that CD13 influences proliferation of monocytoid cells by retarding the velocity of the cell cycle
- CD13/APN is an important target of Ras signaling in angiogenesis and is a limiting factor in angiogenic progression.
- inhibiting CD13/aminopeptidase N on the cell-surface of acute promyelocytic leukemia cells increases ATRA-induced differentiation
- Binding of aminopeptidase N may trigger conformational changes in the coronavirus viral spike protein that facilitate virus entry.
- DPPIV acts synergistically with this protein to regulate T cell function.
- phosphorylation of Ets-2 by RAS/MAPK is a prerequisite for CD13/APN endothelial induction
- CD13 has a characteristic canalicular distribution pattern and may have a role in cell polarization and bile compartmentalization in hepatocellular carcinomas
- The activity of urinary alanine aminopeptidase as an index of alcohol-related kidney impairment is a valuable complement to the existing set of markers of chronic alcohol abuse.
- HCoV-229E(human coronavirus) first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.
- CD13 acts as a signal regulator of FcgammaR function.
- TAL6 and CD13 can form a complex on lung cancer cells; these molecules can modulate cell migration and invasion, and the influence of CD13 on cell motility does not strictly depend on its aminopeptidase activity
- Although hAPN-transgenic mice were resistant to HCoV-229E in vivo, primary embryonic cells and bone marrow dendritic cells were infected in vitro
- results imply a novel functional role of CD13 and Fc gamma receptors as members of a multimeric receptor complex
- Data identify CD13, CD107a, and CD164 as novel basophil-activation antigens.
- Aspirin may exert its anti-atherothrombotic effects in part via the inhibition of thrombin action by up-regulating APN/CD13 on endothelial cells.
- Findings suggest that APN is a multifunctional protein with important roles in vascular endothelial morphogenesis during angiogenesis.
- Mutation in aminopeptidase N (CD13) isolated from a patient suffering from leukemia leads to an arrest in the endoplasmic reticulum
- APN/CD13 regulates TNFalpha-induced apoptosis via inhibition of TNFRI shedding
- bFGF expression upregulates CD13 expression in human melanoma cells and results in enhanced invasive capacity and metastatic behaviour of human melanoma cells.
- Our data provide first evidence for a functional role of DP IV and APN in the sebaceous gland apparatus and for their inhibitors, used alone or in combination, as completely new substances possibly affecting acne pathogenesis in a therapeutic manner.
- The expression of APN/CD13 for patients with NSCLC to be associated with a poor prognosis and angiogenesis. This is the first study to show the relationship between the expression of APN/CD13 and the prognosis of patients with NSCLC.
- RECK protein interacts with MT1-MMP and CD13/aminopeptidase N and modulates their endocytic pathways
- CD13 rapidly processed CXCL11, but not CXCL8, to generate truncated CXCL11 forms that had reduced binding, signaling, and chemotactic properties for lymphocytes and CXCR3- or CXCR7-transfected cells.
- CD13 functions as a novel modulator of signal transduction and cell motility via its influence on specific plasma membrane organization, thus regulating angiogenesis.
- Overexpression of ANPEP is associated with Barrett's adenocarcinomas
- There was a positive correlation between APN/CD13 expression and migratory potential in various ovarian carcinoma cell lines with accordingly enhanced secretion of endogenous MMP-2.
- Expression is frequenty decreased in chronic myelogenous leukemia.
- Finally, exogenous addition of galectin-3 into HUVECs induced angiogenesis in an APN-dependent manner.
- CD13 transcription is regulated by MAF via an atypical response element.
- Enkephalin-degrading enzymes are present in human semen and may be involved in the control of sperm motility, mainly by the regulation of endogenous opioid peptides.
- Data show that aminopeptidase N (APA) plays important roles in the regulation of blood pressure under both the physiological and pathological conditions.
- Data support that APN plays important roles in the regulation of arterial blood pressure and the pathogenesis of hypertension.
- CD13 was expressed in 73% of acute myeloid leukemia patients, CD15 was expressed in 43% of patients, CD33 was expressed in 64% of patients, and CD34 was expressed in 66% of patients.
- APN/CD13 inhibition by Ubenimex enhances radiosensitivity; APN/CD13 plays an important role in tumor progression in several human malignancies
- Data suggest that detection of CD2 or CD13 expression in chronic lymphocytic leukemia (CLL) suggests familial CLL, and that CD38 expression does not carry the negative prognosis observed in sporadic CLL.
- New direction for CD13 biology, where cell surface molecules act as true molecular interfaces that induce and participate in critical inflammatory cell interactions.
- Demonstrates expression of immune system in granulocytes after heart surgery.
- CD13 and/or CD33 are sensitive but not entirely specific markers of anaplastic lymphoma kinase anaplastic large cell lymphomas and should not be misinterpreted as indicating myeloid sarcoma.