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Validated All-in-One™ qPCR Primer for GRIN2A(NM_000833.4) Search again
Product ID:
HQP008371
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EPND, FESD, GluN2A, LKS, NMDAR2A, NR2A
Gene Description:
glutamate ionotropic receptor NMDA type subunit 2A
Target Gene Accession:
NM_000833.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene References into function
- The C-terminus of NR2A is critical for modulation of desensitization by calcineurin.
- The levels of NR2A mRNA are decreased in Alzheimer disease hippocampus and entorhinal cortex compared to controls.
- In schizophrenia, analysis of the GRIN2A gene detected four single nucleotide polymorphisms, and a variable (GT)(n) repeat in the promoter region.
- human cerebral endothelial cells express the message and protein for NMDA receptors.
- N-methyl-d-aspartate receptor subunit messenger ribonucleic acids are differentially expressed during postnatal development of the human hippocampus
- Owing to its role in aspects of cognition, linkage of 4 GRIN2A polymorphisms was studied in inhibitory control, verbal short-term memory and verbal working memory. There was no significant evidence of linkage between GRIN2A and these phenotypes.
- disulfide bridging and structural integrity within the NR1 N-terminal domain is requisite for cell surface N-methyl-D-aspartate receptor expression
- genetic variation in GRIN2A may confer increased risk for attention deficit disorder with hyperactivity
- NR2A subtypes mainly expressed in the striatum, may influence the variability in age of onset of Huntington disease.
- a probable genetic effect for the GRIN2A promoter (GT)n variation on the predisposition to schizophrenia in Japanese cohorts.
- Analysis of the autistic disorder susceptibility locus suggests an association on chromosome 16p between GRIN2A and ABAT.
- the conformation of NR1 subunit homodimers is affected by the partner NR2 subunits during the formation of heteromeric receptor complexes
- GRIN2A and TCERG1 may show true association with residual age of onset for Huntington's disease in genetic association tests in 443 affected people from a large set of kindreds from Venezuela.
- analysis of the role of the glutamate binding pocket in agonist affinity and efficacy of N-methyl-D-aspartate receptor activation
- the three amino acid tail following the TM4 region of the N-methyl-D-aspartate receptor (NR) 2 subunits is sufficient to overcome endoplasmic reticulum retention of NR1-1a subunit
- Our observations suggest a novel mechanism whereby subunit specific changes in the NMDA receptor complex may be linked to chronic anxiety in AD via effects on GlyRS function.
- NR2A(F637) influences NMDA receptor affinity, ion channel gating, and ethanol sensitivity; the changes in NMDA receptor affinity are likely to be the result of altered ion channel gating
- NR2A and NR2B receptor gene variations modify age at onset in Huntington disease in a sex-specific manner.
- NMDAR2A is commonly hypermethylated in primary human colorectal carcinoma and possesses tumor-suppressive activity.
- laser capture microdissection combined with quantitative PCR was used to examine the expression of AMPA (GRIA1-4) and NMDA (GRIN1, 2A and 2B) subunit mRNA levels in Layer II/III and Layer V pyramidal cells in the DLPFC.
- The results of the present study, thus, suggest that blockage of NMDA receptors including the NR2A subunit suppresses MKN45 cell proliferation due to cell cycle arrest at the G(1) phase.
- NR1-1a (D732E)/NR2A and NR1-1a (D723A)/NR2A trafficked as efficiently as NR1-1a/NR2A, there was a 90% decrease in surface expression for NR1-1a (D732A)/NR2A.
- Data show that expression of NMDA receptor NR1, NR2A, and NR2B subunits is significantly lower in brains of cirrhotic alcoholics than in the corresponding areas in both controls and alcoholics without co-morbid disease.