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Validated All-in-One™ qPCR Primer for GRIK2(NM_021956.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.
Gene References into function
- Linkage and association of the glutamate receptor 6 gene with autism
- Specific alleles in GluR6 and CA150 locus were only observed in HD patients.
- describes the time course of the open-channel form of the receptor as a function of glutamate concentration
- Using three single nucleotide polymorphisms in GRIK2 and one in GRIK3, we found no evidence for association with Obssessive-Compulsive disorder in case-control or family-based analyses.
- In this study, the maternal transmission disequilibrium of the glutamate receptor GRIK2 in schizophrenia.
- The modifier effect is actually due to the TAA repeat itself, possibly via a functional consequence on the GRIK2 mRNA.
- Our data on altered functional properties of GluR6(M836I) provide a functional basis for the postulated linkage of GluR6 to autism.
- phosphorylation of the C-terminal tail of GluR6 by PKA leads to potentiation of whole cell response
- These results suggest a potential association between GRIK2 and autism in the Korean population.
- Mutations of GluR6 are unlikely to be associated with autism in the Indian population.
- report a complex mutation in the ionotropic glutamate receptor 6 gene (GRIK2, also called "GLUR6") that cosegregates with moderate-to-severe nonsyndromic autosomal recessive mental retardation in a large, consanguineous Iranian family
- results support the involvement of genes GRIA3 and GRIK2 in antidepressant treatment-emergent suicidal ideation
- the apo state of GluR6 undergoes a cleft closure of 29-30 degrees upon binding full agonists, one of the largest observed in the glutamate receptor family.
- Mutations to GLUR6 binding pocket that selectively affect domoate binding are freported.
- nonconserved residues in GluR6 define the size of the agonist-binding pocket, exerting a steric influence on the bound agonist and the extent of binding-domain closure
- GluR6 C-terminal domain KRIP6 regulates kainate receptors by inhibiting PICK1 modulation via competition or a mutual blocking effect