|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for GPX1(NM_000581.2) Search again
Product ID:
HQP008279
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GPXD, GSHPX1
Gene Description:
glutathione peroxidase 1
Target Gene Accession:
NM_000581.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- role in the inflammatory response in a model of stroke in GPX1 transgenic mice
- protects from CD95-induced apoptosis in cultured breast cancer cells
- Malignant lung tumors (squamous cell carcinoma and adenocarcinoma) had significantly increased levels of this enzyme.
- Results indicate that glutathione peroxidase 1-sensitive reactive oxygen species play an important role in regulation of cell death during cerebral ischemia-reperfusion as well as in brain inflammatory reactions.
- A characteristic polyalanine sequence polymorphism in exon 1 of hgpx1 produces three alleles with five, six or seven alanine (ALA) repeats in this sequence.
- GPx-1 activity condition can downregulate basal 20S proteasome activity
- GPX1 was measured in the blood from patients with diabetes mellitus, insulin-dependent.
- The GPx-1 Leu-containing codon 198 allele was more frequently seen in breast cancer than the Pro-containing one. LOH at this locus occurred in ~36% of the breast tumors. GPx-1 allelic identity & LOH have a role as factors in breast cancer development.
- GPX1 overexpression delays cell growth and protects them from GSH and H(2)O(2) toxicity
- PU.1 directly regulated the expression of only the glutathione peroxidase gene through binding sites in the promoter and a 3' regulatory region.
- c-Abl and Arg associate with glutathione peroxidase 1 (GPx1), and this interaction is regulated by intracellular oxidant levels. The c-Abl and Arg SH3 domains bind directly to a proline-rich site in GPx1 at amino acids 132-145.
- GPX1 inhibits 5-lipoxygenase in human blood cells
- Normal pregnancy is associated with increased glutathione peroxidase activity and insulin resistance. Potential link among antioxidant defenses, insulin resistance, and dietary fat intake.
- Genetic manipulation of islet beta cells to increase expression of Gpx-1 may protect them from oxidative injury associated with the transplantation procedure.
- High glutathione peroxidase activity in human alveolar macrophages limits the effectiveness of H2O2 to act as a mediator of inflammatory gene expression.
- No statistically significant differences for GPx-1, phospholipid hydroperoxide glutathione peroxidase, and glutathione reductase were encountered between normal values and those of asthenozoospermic patients.
- Akt and P70S6K phosphorylation and Gadd45 levels are modulated by GPx-1 in tumor cells
- The GPX1 Pro/Leu genotype may significantly increase the risk of bladder cancer and the increased risk may be modified by the Ala-9Val MnSOD polymorphism. The GPX1 genotype may further affect the disease status of bladder cancer.
- the immature brain has limited GPX activity and is more susceptible to oxidative damage and may explain the paradoxical effect seen in ischemic neonatal brain when SOD1 is overexpressed.
- There is no evidence between GPX1 polymorphism and the risk of basal cell carcinoma.
- GPX1 polymorphism is unlikely to be associated with susceptibility to schizophrenia.
- functional variants in the GPx-1 gene are associated with increased intima-media thickness of carotid arteries and risk of cardiovascular and peripheral vascular diseases in type 2 diabetic patients.
- The functional polymorphism (Pro198Leu) in he GPX1 gene might be associated with openness to experience among the personality traits.
- overexpressing an antioxidant gene such as GPX1 in endothelial cells is able to change the basal mRNA and protein Bax levels without affecting those of p53 and Bcl-2
- glutathione peroxidase-1 activity is decreased by aminoglycosides through interference with selenocysteine incorporation
- NF-kappaB is upregulated in the Gpx1(-/-) mouse, and this upregulation contributes to the increased cell death seen in the Gpx1(-/-) after middle cerebral artery occlusion.
- associations between several smoking variables and alcohol intake and lung cancer risk among GPX1(TT) carriers than among GPX1(CC) and GPX1(CT) carriers
- Polymorphisms in the GPX-1 and MnSOD genes may have a role in development of breast cancer
- We have been able to correlate embryo morphology on day 3 with the sperm expression of GPX family members.
- Role for selenium in risk of lung cancer and independent regulation of GPX1 in a tumor cell line.
- These findings suggest that GPx-1 inhibits UVA-induced AP-2alpha expression by suppressing the accumulation of H(2)O(2).
- Systemic activity of the enzymatic antioxidants (CuZn/SOD, MnSOD, GSH-Px, and CAT) as well as level of lipid peroxidation determined by MDA may not be increased in the course of immune-inflammatory processes associated with chronic idiopathic urticaria.
- the C --> T polymorphism of GPX1 genotype was independently associated with in-stent restenosis in a Japanese population
- Selenium and GPx-1 overexpression protect mammalian cells against UV-induced DNA damage
- The presence of Pro197Leu substitution of the GPx-1 gene may play a crucial role in determining genetic susceptibility to coronary-arteriosclerosis in type 2 diabetes.
- study shows the well-documented CAT exon 9 T/C & GPX 1 codon 200 polymorphisms may not be associated with Gujarat vitiligo patients; results suggest presence of novel SNPs in Gujarat vitiligo population
- docosahexaenoic acid sensitizes breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response
- T-Allel of GPX1(Pro200Leu)plays a protective role in early onset lung cancer susceptibility
- found a positive association between the studied GPX1 polymorphism and lobar PICH in a Polish population.
- No significant risk for GPX1 in colorectal adenoma.
- GPX1 Pro198Leu variants are associated with the prevalence of metabolic syndrome in Japanese men but not in women.
- The variant allele of THBS2 is a risk factor for TAA in hypertensive patients, whereas the variant alleles of HSPA8, GPX1, AGT, and TNF are protective against this condition.
- Glutathione-peroxidase was increased in benign hyperplasia of prostate and decreased in prostate cancer.
- Data show that the cellular distribution of GPx-1 has shown it to be in highest levels in microglia, lower levels in neurons, a trace amount in astrocytes, and undetectable in oligodendrocytes in Parkinson's disease and dementia with Lewy bodies tissues.
- acute myocardial infarction incidence significantly increased in patients with a concomitant decrease in plasma vitamin E and erythrocytic GPX-1 activity.