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Validated All-in-One™ qPCR Primer for GRK5(NM_005308.2) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq].
Gene References into function
- Human substance P receptor undergoes agonist-dependent phosphorylation by G protein-coupled receptor kinase 5 in vitro.
- Data indicate that GRK5 does not regulate the sorting of human beta 2-adrenoceptors in the endocytic pathway.
- a group of hydrophobic amino acids within the membrane binding motif is critical to mediating the PM localization of GRK5
- GRK5 has a DNA-binding nuclear localization sequence
- High expression was detected in septic neutrophils and control cells treated with cytokines plus LPS.
- Results show that GRK5 plays a distinctive role in the phosphorylation of the beta2AR.
- GRK5-Leu41 represents a gain-of-function polymorphism that evokes enhanced loss-of-function of beta2AR during persistent agonist exposure, and thus may contribute to beta-agonist variability in asthma treatment of African-Americans.
- Protective effect of a GRK5 polymorphism on heart failure and its interaction with beta-adrenergic receptor antagonists is reported.
- GRK5 overexpression causes nuclear accumulation of IkappaB alpha, leading to the inhibition of NFkappaB transcriptional activity.
- Reciprocity of regulation between platelet-derived growth factor receptor beta (PDGFRbeta) and GRK5, or their activation/deactivation cycle, mirrors other reciprocal regulatory mechanisms affecting tyrosine kinases.