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Validated All-in-One™ qPCR Primer for ANGPT2(NM_001147.2) Search again
Product ID:
HQP008202
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AGPT2, ANG2, LMPHM10
Gene Description:
angiopoietin 2
Target Gene Accession:
NM_001147.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2
- ang-2 is expressed in areas undergoing vascular remodeling and is involved in neovascularization.
- results indicate that there is a difference in the Ang/Tie2 gene expression between physiological and pathological angiogenesis in the ovary.
- angiopoietin-2 is selectively induced in cultured human umbilical vein endothelial cells by hyperbaric oxygen treatment
- may play important roles in placental biology and chorionic villus vascular development and remodeling in an autocrine/paracrine manner
- In focal nodular hyperplasia, Ang-1 was significantly up-regulated, Ang-2 was down-regulated, and the Ang-1/Ang-2 ratio was highly and specifically increased.
- an increased expression of Ang-2/1 in the presence of VEGF may play a critical role in promoting tumor angiogenesis and progression in human hepatocellular carcinoma
- Tumor-derived VEGF significantly up-regulated the expression of Ang-2 in host stroma endothelial cells, resulting in markedly increased Ang-2/Tie-2 mRNA copy number ratio in vivo.
- plays a critical role in inducing tumor cell infiltration, and this invasive phenotype is caused by activation of MMP-2
- High-level expression of angiopoietin-2 and VEGF receptors observed in endothelium of verruga peruana. Infection of cultured endothelium with B. bacilliformis also resulted in induction of angiopoetin-2 in vitro.
- No association between mutant allele and the occurrence of idiopathic recurrent miscarriage. No statistically significant differences with respect to allele frequencies were observed.
- Ang2, PIGF and VEGF-C play a role in promote endothelial survival and vascular remodeling by human cytotrophoblast.
- hypoxia-induced Ang2 expression is regulated by COX-2-dependent prostanoids
- Angiopoietin 2 induced STAT5 activation and p21waf expression and increased the fraction of endothelial cells in G1.
- angiopoietin-2 and VEGF at the deepest invasive tumor site may have roles in tumor angiogenesis
- increase in ANG2 RNA from peripheral tumor to the intermediate portion, and decrease in recruitment of periendothelial supporting cells around the vascular endothelial, suggests that Ang-2 may play a role in the immaturity of vessels in liver neoplasm
- Ang-2 is a stored, rapidly available molecule in endothelial cells suggesting that the angiopoietin/Tie-2 system during angiogenesis likely to be involved in rapid vascular homeostatic reactions such as inflammation and coagulation.
- The autocrine/paracrine signaling of the Ang/Tie2 system is important for the up-regulated angiogenesis in the RA synovium, as well as for synoviocyte behavior, by regulating chemotactic cell movement.
- Protein kinase C and protein kinase A activators increased the mRNA levels of ANGPT-2 in human granulosa cells. Role of both PKA and PKC dependent signaling cascades in the regulation of ANGPT-2 mRNA.
- Transcription factor Ets-1 has a role in transactivation of the angiopoietin 2 promotor.
- increased expression of Angiopoietin-1 and Angiopoietin-2 play a critical role in the process of vascular development in HCC
- Data show that angiopoietin-2, coordinated with VEGF, may play a role in regulating tumor angiogenesis in gastric cancer.
- in granulosa cells the mRNA of various growth factors is detectable by RT-PCR and that VEGF-A and ANG2 is regulated by the gonadotropic hormone choriogonadotropin
- Thrombin reduces expression of Ang-2 protein and mRNA expression in human endometrial stromal cells.
- the angiopoietin/Tie-2 system may participate in the angiogenic response to hypoxia in renal tissues and in tumor angiogenesis in renal carcinoma.
- angiopoietins -1 and 2 have roles in endothelial development
- Under non-reducing conditions, angiopoietin 2 forms disulfide-linked dimers.
- Overexpression of Ang-2 mRNA is associated with non-small cell lung cancer
- Ang2 demonstrates proinflmmatory potential by inducing endothelial P-selectin translocation and neutrophil adhesion onto vascular endothelial cells.
- systemic administration of ang2-selective inhibitors will suppress tumor angiogenesis, supporting the idea that Ang2 plays a proangiogenic role postnatally
- Plasma levels in type 2 diabetes are selectively elevated in patients with diabetes and are associated with indexes of endothelial damage/dysfunction.
- Expression and purification of recombinant ANGPT2 produced in CHO cells was studied.
- Ang2 overexpression may play a key role in placental vascular remodelling.
- up-regulation of Ang2, MMP-2, MT1-MMP, and LN 5 gamma 2 is associated with the invasiveness displayed by human gliomas
- Ang-2 (but not Ang-1) was higher in patients with diabetes compared to controls (p<0.01), with no significant difference between patients with and without cardiovascular diseases
- Tie-2 receptor is expressed by human neutrophils whose active site ligation with either angiopoietin-1 or angiopoietin-2 exerts migratory effects on the one hand and arrests VEGF-mediated chemotaxis on the other
- We show for the first time that Ang2 causes a marked stimulation of EPC migration.
- Measurement of serum Ang-2 concentration in pregnant women may serve as a useful marker in the diagnosis and potentially in predicting subsequent development of preeclampsia.
- A3 receptor stimulation activates p44/p42 and p38 mitogen-activated protein kinases, which are required for A3-induced increase of HIF-1alpha and Ang-2
- an increased expression of Ang-2/1 in the presence of VEGF may play a critical role in promoting tumor angiogenesis and progression in human hepatocellular carcinoma
- hypoxia-elevated Ang-2 expression in gastric cancer cells may be mediated by both Cox-2-derived PGE2 and HIF-1alpha pathways
- These results identify a critical role for Ang-2 in disrupting normal pulmonary endothelial function.
- data establish a functional link between Ang2 interaction with alpha(v)beta(1) integrin and glioma cell invasion through the FAK/p130(Cas)/ERK1/2 and JNK-mediated signaling pathway
- Participation of up regulated angiopoietin-2 in the placental tissues may indicate a role for this angiogenic factor in placenta accreta
- Ang-2 expression was associated with a significantly higher level of microvessel density and might play a proangiogenic role in cholangiocellular carcinoma.
- Hypoxia-induced Ang-2 mRNA expression was clearly evident in HCT116 cells that did not express Ang-2 under normoxic conditions.
- VEGF and Ang-2 may have roles in microvessel growth in invasive ductal carcinoma of the breast
- expression of Ang-1 and Ang-2 is important for gastric tumor angiogenesis, and a possible autocrine/paracrine function of the angiopoietin/Tie2 system is involved in gastric cancer progression
- Expression of VEGF and Ang-2 correlated with microvessel density. Strong Ang-2 expression and/or high nuclear expression of HIF-1alpha is a significant predictive factor for recurrence after curative resection in hepatocellular carcinoma patients.
- Common protective and diverse smooth muscle cell effects of Ang1 and Ang2 in cardiac allograft microenvironment and importance of timing of angiopoietins to achieve therapeutic effects.
- These results suggest that Ang-2 promotes endothelial cell survival through the ERK1/2 and PI3-kinase pathways and that this angiopoietin is not a strong promoter of endothelial cell migration.
- Results suggest that the eutopic endometrium from endometriosis patients is more angiogenic and prone to growth because of higher Ang-2 mRNA expression than the endometrium from women without endometriosis.
- deregulated Ang2 expression during gliomagenesis hinders successful angiogenesis
- PAR-1-induced proliferation of endothelial progenitor cells involves angiopoietin 2
- Dominant Ang-2 expression against Ang-1 through Tie2 receptor in the presence of VEGF plays a critical role in initiating early neovascularization and transformation of noncancerous liver to hepatocellular carcinoma.
- there is no association between carrier state of Ang2(-35) and risk of ROP in preterm infants.
- the alteration of angiopoietin balance in favor of Ang-2 may play a critical role in the pathophysiology of AML
- sAng-2 and sTie-2 play a role in progression of some haematological malignancies
- Circulating levels of Ang-2 and sTie-2 receptor were detectable but invariant in women during COS cycles.
- Hepatitis B virus X protein transactivator could account for the induction of Ang2 observed in chronic hepatitis B.
- Data demonstrate that angiopoietin-2 expression is rapidly induced in endothelial cells by the transcription factor FOXO1 after inhibition of the phosphatidylinositol 3-kinase/Akt pathway, and that Ang-2 acts as a Tie2 agonist.
- The higher expression of Hpa and Ang-2 in ectopic and eutopic endometrium may play an important role in the pathogenesis and development of endometriosis.
- High angiopoietin 2 is associated with carcinogenesis and portal vein tumor thrombus formation in human hepatocellular carcinoma
- Ang2 is involved in pericyte recruitment, and modulates intraretinal, and preretinal vessel formation in the eye under physiological and pathological conditions
- Foxo-1 and Ang-2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis.
- KSHV activated the Ang-2 promoter via AP-1 and Ets1 transcriptional factors, which were mediated by ERK, JNK, and p38 mitogen-activated protein kinase (MAPK) pathways.
- the relationship of angiopoietin-1, angiopoietin-2 and tie-2 to coronary collateral formation in patients with coronary artery disease
- study demonstrates that mesenteric venous, arterial, and lymphatic endothelial cells responded differentially to Ang1 and Ang2; results suggest cellular responses to Ang1 and Ang2 stimulation vary depending on the origin of the endothelial cells
- study of the relationship between Ang-2 expression, immunoglobulin (Ig) mutational status and other established prognostic markers as well as progression-free survival in B-cell chronic lymphocytic leukemia
- Ang-2 is a key regulator of several essential phases of wound healing.
- The serum sFlt-1/Ang-2 ratio is significantly elevated in preeclamptic women as compared to healthy pregnant women.
- Serum angiopoietin-2 is a useful clinical marker for detecting non-small cell lung cancer with distant metastasis and is of potential prognostic value.
- Angiopoietin-2 may recruit Tie-2-expressing monocytes to tumors and sites of inflammation, where it then plays an important role in modulating cytokines implicated in angiogenic and inflammatory processes.
- Although no immediate effects were observed in soluble VEGF levels, percutaneous coronary intervention affected intracardiac angiopoietin-1 with a systemic release of angiopoietin-2
- Hypoxia stimulates protumor functions of macrophages and stimulates the release of ANGP2 from breast tumor cells and blood vessels.
- Single nucleotide polymorphisms are not related to ovarian, cervical or endometrial cancer.
- Ang2 produced by oscillatory shear stress in endothelial cells plays a critical role in migration/tubule formation. May have role in diseases with disturbed flow/angiogenesis.
- Regulates vascular endothelial growth factor expression at the transcriptional level in relation to a decrease in hypoxia-inducible factor 1 alpha (HIF) expression and HIF-DNA-binding activity.
- Results describe the expression of angiopoietin-1, 2 and 4 and Tie-1 and 2 in gastrointestinal stromal tumors, leiomyomas and schwannomas.
- Increased expression of Ang-2 may be responsible for blood vessel formation and rapid growth of the colorectal adenocarcinoma.
- High Angiopoietin-2 levels are associated with acute myeloid leukemia
- Increased expression of angiopoietin-2 is associated with early B-cell chronic lymphocytic leukemia
- Angiopoietin-2 levels in the hepatic vein as a useful predictor of tumor invasiveness and prognosis in human hepatocellular carcinoma.
- Local Ang-2 release from AML cells is less common than Ang-1 release, but high systemic levels of Ang-2 may affect bone marrow angioregulation.
- AMPK-dependent phosphorylation and degradation of FoxO1a attenuates Ang-2 expression and protects against the pro-inflammatory actions of TNF-alpha.
- Data show plasma angpt-2 levels were significantly elevated in children with septic shock when compared with healthy children, as well as critically ill children with either SIRS or sepsis.
- Plaque Ang-2 levels are associated with MMP-2 activity. Ang-2-induced MMP-2 activity might play a role in the development of (unstable) plaque microvessels.
- endotoxemia triggers functional inhibition of the Ang-1/Tie-2 receptor pathway by reducing Ang-1 and Tie-2 expression and inducing Ang-2 levels and that this response may contribute to enhanced vascular leakage in sepsis
- Among patients with hypertension, raised levels of Ang-2 were predictive of MI.
- Ang-2 may be a readily available powerful biomarker to pre-estimate disease-free survival after allogeneic stem cell transplantation in patients with high-risk myeloid malignancies.
- in children Angpt-2 levels correlated significantly with cardiac intensive care unit (CICU) length of stay (LOS) and were an independent predictor for CICU LOS
- patients with high-grade GVHD(graft versus host disease) exhibited a significant increase in Ang-2(angiopoietin 2) compared to patients with low-grade GVHD
- Ang-2 was also found predominantly in syncytiotrophoblasts of the very early human placenta with lower immunostaining levels evident in cytotrophoblasts. Moderate immunoreactivity for Ang-2 was observed in endothelial cells, ACC and Hofbauer cells.
- Hypoxic regulation of Ang-2 is HIF-dependent and demonstrate that HIF-1alpha binds in human microvascular endothelial cells (HMVEC) to an evolutionary conserved Hypoxia-Responsive Element (HRE) located in the first intron of the Ang-2 gene.
- Data show that serum levels of angiopoietin-2 in multi-trauma patients are increased upon advent of septic complications and they are connected with bad prognosis.
- The longitudinal relationship between Angiopoietin-2 and endothelial function was assessed by using a mixed-effects model. Ang-2 concentrations were elevated in severe falciparum malaria.
- These findings suggest that the Ang and VEGF families may play a role in the process of spiral artery remodelling in normal pregnancy.
- Angiopoietin-2 exocytosis is stimulated by sphingosine-1-phosphate in human blood and lymphatic endothelial cells.