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Validated All-in-One™ qPCR Primer for DLL1(NM_005618.3) Search again
Product ID:
HQP008111
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
DELTA1, DL1, Delta, NEDBAS
Gene Description:
delta like canonical Notch ligand 1
Target Gene Accession:
NM_005618.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq].
Gene References into function
- Delta-1 can enhance myeloid and lymphoid marrow-repopulating ability and promote the generation of thymus-repopulating T cell precursors.
- suppresses the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines
- Dll1 is a substrate for regulated intramembrane proteolysis, and its intracellular region possibly fulfills a specific function in the nucleus
- Delta and Jagged undergo ADAM-mediated ectodomain processing followed by PS-mediated intramembrane proteolysis to release signaling fragments
- induces a NIH 3T3 cell tranformed phenotype mediated by FGF signaling.
- Delta-1 and Jagged-1 have roles in growth suppression in two myeloid leukemia cell lines
- Notch ligand, Delta-1, reduced the TNF-alpha-induced growth suppression and apoptosis in U937 cells.
- Dlk may regulate the endothelial function in neuroblastic tumors.
- Down-regulation of Notch-1, Delta-like-1, or Jagged-1 by RNA interference induces apoptosis and inhibits proliferation in multiple glioma cell lines.
- Density of the Notch ligand Delta1 determines generation of B and T cell precursors from hematopoietic stem cells.
- When a dominant-negative form of kuzmanian in transgenic mice impairs Notch signaling in receiving T-cells, increasing Delta-1 on sending cells overcomes this defect.
- Dll1 is presented on the surface of AJs formed at the apical termini of processes through interaction with MAGI1 to activate Notch on neighboring cells in the developing central nervous system
- culture with increased amounts of Delta1(ext-IgG) induced apoptosis of CD34+ precursors resulting in decreased cell numbers, without affecting generation of CD7+ cells
- The expression of the intracellular domain of Delta1 results in a non-proliferating senescent-like cell phenotype which is dependent on the expression of the cell cycle inhibitor, p21.
- Data describe the immunohistochemical staining pattern of four Notch receptors (Notch1-4) and their ligands (Delta1 and Jagged1) in synovial tissues obtained from rheumatoid arthritis patients.
- DL1-induced activation of the Notch1 pathway controls the lineage commitment of early thymic precursors by altering the levels between Spi-B and GATA-3.
- Dll1 was expressed in arterial endothelial cells, not venous Ecs or capillaries. Notch signaling by induction of Dll1 is necessary & sufficient to regulate ephrin-B2 expression & EphB2- & EphB4-dependent branching morphogenesis in arterial EC.
- Tbx18 interacts with Gata4 and Nkx2-5 and competes Tbx5-mediated activation of the cardiac Natriuretic peptide precursor type a-promoter. Tbx18 down-regulates Tbx6-activated Delta-like 1 expression in the somitic mesoderm in vivo
- Delta-like protein seems to be a highly sensitive and specific marker for hepatoblastomas.
- Report the antitumor effects of COX2 inhibitors may be associated with effects on the Delta1/Notch1 pathway in colon tumor cells.
- The intracellular region of Notch ligands Dll1 and Dll3 regulates their trafficking and signaling activity