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Validated All-in-One™ qPCR Primer for CXCR3(NM_001504.1) Search again
Product ID:
HQP007900
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR
Gene Description:
C-X-C motif chemokine receptor 3
Target Gene Accession:
NM_001504.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues.
Gene References into function
- chemokine/CXCR3 activity
- downregulation following T cell-endothelial cell contact
- Graves' disease is associated with an altered CXCR3 expression in thyroid-derived compared to peripheral blood t lymphocytes
- Expression of CXCR3 on CD4+ and CD8+ T cells was significantly reduced after 3 months of interferon beta-1a treatment in multiple sclerosis.
- These findings suggest that the CXCR3/CXCL10 axis may be involved in the T cell recruitment that occurs in peripheral airways of smokers with COPD and that these T cells may have a type-1 profile.
- Most Natural killer t-cells express receptors for extralymphoid tissue or inflammation-related chemokines (CCR2, CCR5, and CXCR3), while few NKT cells express lymphoid tissue-homing chemokine receptors (CCR7 and CXCR5).
- Expression of CXCR3 ligands (MIG, IP-10, and ITAC) in the bronchoalveolar lavage fluid of transplantation recipients is associated with persistent recruitment of mononuclear cells, a pivotal event in the pathogenesis of bronchiolitis obliterans syndrome.
- Gene expression is predictive for the individual response of children with chronic allograft nephropathy to mycophenolate mofetil.
- Significant increase in surface CCR5 in CD4+, CD8+, CD19+ and CD14+ cells as well as an increased percentage of CXCR3 and CXCR4 in CD14+ cells in MS patients.
- migration of murine and human IFN-producing cells to CXCR3 ligands in vitro requires engagement of CXCR4 by CXCL12
- Activation of the CXCR3 receptor in proximal tubular cells might disturb natriuresis during inflammatory and ischemic kidney disease via early growth response gene-1-mediated imbalance of reactive oxygen species.
- CXCR3-Mig-coexpressing lymphoma cells were abundant in high MALT of the stomach and thyroid, but rare in other subtypes
- CXCR3-targeting chemokines control T-cell migration via PTX-sensitive, phospholipase C pathways and phosphatidylinositol kinases other than class I PI3Kgamma.
- alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth
- Data suggest that in oral lichen planus, the presence of CCL5 and CXCL10 in the cytolytic granules of tissue-infiltrating CD8(+)T cells expressing CCR5 and CXCR3 reveals a potential self-recruiting mechanism involving activated effector cytotoxic T cells
- eosinophil responses mediated by chemokines acting at CCR3 may be regulated by two distinct mechanisms: the antagonistic effects of CXCR3 ligands and the sequestration of CCL11 by CXCR3-expressing cells
- Targenic of cxcr3 and/or ICOS may have clinical application in the prevention and treatment of chronic allograft nephropathy.
- Ligands control plasmacytoid dendritic cell responsiveness to the constitutive chemokine (SDF-1)/CXCL12.
- In vitro chemotaxis induced by this chemokine is not necessarily predictive of its in vivo lymphocyte-recruiting activity.
- CXCR3 functions broadly--on recently activated as well as fully differentiated CD4+ T cells and on cells that participate in the germinal center reaction as well as on those that infiltrate peripheral inflammatory sites.
- In restinosis, increased plasma concentrations of IP10 were accompanied by a compensatory decrease in the CXCR3 expression on lymphocytes, but not monocytes, suggesting that a high plasma concentration of IP10 strongly induces monocytes signaling.
- Data demonstrate a significant age-dependent difference in the response of osteoblasts to CXCR3 and CXCR5 activation.
- CXCR3-ligation preferentially augments ongoing Th1 over Th2 responses and suggest that reduced capacity of allergic individuals to respond to CXCR3 ligands promotes the maintenance of human allergic disorders
- interstitial CXCR3 may play an important role during progressive loss of renal function
- Mig and IP-10 may be involved in the recruitment of natural killer cells or other phenomena in the human endometrium.
- Intracellular domains of CXCR3 mediate CXCL9, CXCL10, and CXCL11 function
- autocrine activation of CXCR3 expressed by epithelial cells may contribute to airway inflammation and remodeling in obstructive lung disease by regulating cell migration.
- plays an important role in lymphocyte trafficking to CSF during HIV-1 infection
- CXCR3-deficient mice demonstrated increased mortality with progressive interstitial fibrosis, and a reduced early burst of IFN-g, upon injury. CXCR3 deficiency results a deficiency in lung NK cells.
- The CXCR3 ligands IP-10/CXCL10 & Mig/CXCL9 revealed a proproliferative effect but did not influence apoptosis of mesangial cells, suggesting involvement in processes involved in renal inflammation, regeneration and glomerular homeostasis.
- CXCR3 and CCR4 were heterogeneously expressed in peripheral T-cell lymphomas.
- CXCR3 is the principal inflammatory chemokine receptor involved in Behcet's disease
- A splice variant of CXCR3 despite its severe structural changes still localizes to the cell surface and mediates functional activity of CXCL11.
- LMP1-mediated p38/SAPK2 activation induces expression of the chemokine IP-10 and regulates transcript stability
- Once induced in memory B cells, CXCR3 expression remains part of the individual cellular memory
- studies suggest that IRBP and S-Ag can initiate innate and, in sensitive individuals, adaptive immune response by attracting iDCs and T and B cells expressing CXCR3 and CXCR5
- These data indicate that IFN-gamma mediates the recruitment of lymphocytes into the lung via production of the chemokine CXCL10, resulting in Tc1-cell alveolitis and granuloma formation.
- Marked up-regulation of IP-10 may predict the initial graft injury and the onset of delayed graft function in kidney transplantation.
- CXCR3/IP-10 signaling is associated with the pathogenesis of human myasthenia gravis.
- recruitment of T cells expressing Cxcr3 into the invasive margin of colorectal cancer.
- These results suggest that inhibition of the CXCL10/CXCR3 axis offers a novel target for the treatment of asthma.
- Expression of chemokine receptor CXCR3 mediates the selective accumulation of memory CD4+ T-cells into the cerebrospinal fluid of patients with neuroborreliosis.
- These data suggest that the regulation of the CCL2 and CXCL10 expression exhibit significant differences in their mechanisms, and also demonstrate that the alveolar epithelium contributes to the cytokine milieu of the lung.
- CXCR3 plays an important role in controlling inflammation of the central nervous system in transgenic mice during intracerebral infection with noncytolytic lymphocytic choriomeningitis virus
- Our findings suggest that polymorphisms in CXCR3 might be one of the genetic factors for the risk of asthma development, especially in male atopic subjects.
- Activation of CXCR 3 promotes lymphocyte adhesion and transendothelial migration under flow.
- The frequency of CXCR3-expressing cells in peripheral blood is significantly higher in primary biliary cirrhosis, and CXCR3-positive cells are also found in the portal areas of diseased livers, primarily on CD4-positive T cells.
- Activation of CXCR3 exerts a mitogenic effect in human bronchial epithelial cells.
- CXCL11-dependent CXCR3 internalization and cell migration are regulated by the CXCR3 membrane proximal carboxyl terminus, whereas adhesion is regulated by the 3i loop S245.
- demonstrate an intimate functional relationship among CD4, CCR5, and CXCR3, in which CCR5 can act as an adaptor molecule for CD4 signaling
- epithelial CXCR3 may be involved in postsecretion regulation of chemokine bioavailability
- Patients who had a combined BM relapse had lower IL-8 and CXCR4 expression than those who had an isolated BM relapse om chldhood acute lymphoblastic leukemia.
- Double immunofluorescense staining demonstrated that cellular sources of MCP-1/CCL2 and IP-10/CXCL10 were hypertrophic astrocytes and that both astrocytes and microglia/macrophages expressed CCR2 and CXCR3.
- IP-10 does not seem to be a risk factor for Alzheimer disease, but a novel rare polymorphism has been identified, which could exert a role in susceptibility.
- Data demonstrate that chemokine activation of CXCR3 involves both high-affinity ligand-binding interactions in the extracellular domains of CXCR3 and a lower-affinity receptor-activating interaction in the second extracellular loop.
- These results together indicate that the expression of CXCR3 is up-regulated on intermediately differentiated memory CD8+ T cells. CXCR3high CD8+ T cells had a greater ability to migrate in response to CXCR3 ligands than CXCR3low ones.
- analysis of human CXCR3 receptor agonists
- These results indicated that CXCL10 inhibited LNCaP cell proliferation and decreased PSA production by up-regulation of CXCR3 receptor. CXCL10 may be potentially useful in the treatment of prostate cancer.
- Activation of Ras plays a critical role in modulating the expression of both CXCL10 and CXCR3-B, which may have important consequences in the development of breast tumors through cancer cell proliferation.
- most circulating human CD4+ T cells store the inflammatory chemokine receptors CXCR3 and CXCR1 within a distinct intracellular compartment
- CXCR3 was expressed in EEC, ESC, and trophoblast cells. Addition of IFN-gamma to EEC increased the chemotactic activity of its culture medium to trophoblast cells and T cells, and the effect was suppressed by Abs of three CXCR3 ligands
- mutant receptor (CXCR3[K300A, S304E]) showed markedly enhanced HIV coreceptor function compared to the wild-type receptor (CXCR3[WT])
- Low expression of CXCR3 by blood CLL B cells was a clear, independent predictor of death, suggesting that low CXCR3 expression has a role in the invasive capacity and progression of this tumor.
- CD13 rapidly processed CXCL11, but not CXCL8, to generate truncated CXCL11 forms that had reduced binding, signaling, and chemotactic properties for lymphocytes and CXCR3- or CXCR7-transfected cells.
- CXCR3 expression on mononuclear cells and CXCR3 ligands in renal cell carcinoma is increased in response to systemic IL-2 therapy
- In chronic hepatitis C, anti-viral treatment induces an increase in CD8+ cells expressing chemokine receptors. Viral control is associated with an increase in CXCR3(high) expressing CD8+ cells during treatment.
- T cells expressing CCR6, CXCR3, and CXCR6 act coordinately with respective ligands and Th1 inflammatory cytokines in the alveolitic/granuloma phases of the disease.
- CXCR3 was expressed strongly at the acinar cell membrane in chronic pancreatitis as compared to normal
- IKK2 is also involved in the IFN-gamma-stimulated release of the CXCR3 ligands through a novel mechanism that is independent NF-kappaB
- In clinically inflamed dental pulp, CXCR3 expression was observed mainly on T-cells.
- Human chemokine receptor CXCR3 (CXCR3-B) transduces apoptotic but not chemotactic signals in microvascular endothelial cells following exposure to high concentrations of CXCL4.
- CXCR3 <-> ligand-mediated lymphocyte recruitment is involved in cutaneous lichenoid graft-versus-host disease.
- Aim of this study was to elucidate the effects of CXCR3-B on activation of members of the mitogen-activated protein kinase family, and to explore the relevance of defined signaling pathways to the angiostatic effects of CXCR3-B ligands.
- CXCR3 receptor is expressed by human type II pneumocytes, and the CXCR3-A splice variant mediates chemotactic responses possibly through Ca(2+) activation of both mitogen-activated protein kinase and PI 3-kinase signaling pathways.
- the activities of CXCR3 are tightly controlled following mRNA translation. such tight regulation of CXCR3 may serve as a control to avoid the unnecessary amplification of activated T lymphocyte recruitment.
- Gliadin binds to CXCR3 and leads to MyD88-dependent zonulin release and increased intestinal permeability
- Expression of CXCR3 was significantly associated with lymph node metastasis in lung adenocarcinoma
- We showed that CXCR3 expression was an independent predictive factor for non-responsiveness to H. pylori eradication therapy in patients with gastric MALT lymphoma.
- study revealed that surface expression of the chemokine receptors CXCR3 and CCR5 on CD4+ T cells were increased markedly in patients with active SLE more than SLE patients in remission and the healthy subjects
- IFN-alpha2b possibly controls chemotaxis by regulating the interaction between CXCL10 and CXCR3A
- CXCR3-associated chemokines may play an important role in the development of necroinflammation and fibrosis in the liver.parenchyma in chronic hepatitis C infection.
- Calcineurin inhibitors modulate CXCR3 splice variant expression and mediate renal cancer progression
- rs2280964A allele significantly correlates with decreased CXCR3 gene expression, which would lead to variation in immune cell responses to chemokine-cytokine signals in vitro and ex vivo that includes a decrease in chemotactic activity.
- infiltrated neutrophils from patients with chronic inflammatory lung diseases and rheumatoid arthritis highly express CCR1, CCR2, CCR3, CCR5, CXCR3, and CXCR4
- ratio of CCR4+/CXCR3+ cells was 4.45 in chemotherapy and 0.72 in immunotherapy