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Validated All-in-One™ qPCR Primer for GP5(NM_004488.2) Search again

Product ID:
HQP007809 (click here to view gene annotation page)
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Species:
Human
Symbol:
GP5
Alias:
CD42d, GPV
Gene Description:
glycoprotein V platelet
Target Gene Accession:
NM_004488.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
 
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Buy Catalog# Description Note Price (US$)
HQP007809 All-in-One™ qPCR Primer for NM_004488.2 (200 reactions) GP5 inquire
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Validated result:

AMP Melt
A B
Figure: All-in-One™ qPCR Primer Validated result to GP5.

Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 193400) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis.


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