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Validated All-in-One™ qPCR Primer for GNAI1(NM_002069.5) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]).
Gene References into function
- Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits
- co-stimulation of G(12/13) and G(i) pathways is sufficient to activate GPIIb/IIIa in human platelets in a mechanism that involves intracellular calcium
- An age-induced increase in G alpha i may have a role in depressing cardiac function in aged human atria.
- region of the third cytoplasmic loop of Dopamine D2 receptor is crucial for determining G(i) protein coupling specificity.
- Gi has a role in insulin attenuation of platelet functions by interfering with cAMP suppression along with IRS-1
- Gi, but not Gq or G12/13, signaling pathways are required for activation of Akt in platelets
- Gi has a role in CXCL16 signaling that induces cell-cell adhesion and aortic smooth muscle cell proliferation
- the G(alpha)o/i-coupled cannabinoid receptor, by regulating the proteasomal degradation of Rap1GAPII, activates Rap1 to induce neurite outgrowth.
- G12, Rho, filamin-A, and the actin cytoskeleton are required for amino acid-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor
- TPO integrates G(i), but not G(q), stimulation, supports integrin alpha(IIb)beta(3) activation platelet aggregation independently of phospholipase C but requires PI3-kinase and Rap1B
- Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors.
- Nef protein of human immunodeficiency virus (HIV) reduces cell surface levels of eight different members of the CC- and CXC-family of Chemokine receptors (CKRs) by up to 92%.
- autotaxin induces uPA expression via the Gi-PI3K-Akt-NF-kappaB signaling pathway that might be critical for autotaxin-induced tumor cell invasion and metastasis
- These data revealed that PAR1 can be part of a preassembled complex with Galpha(i1) protein, resulting either from a direct interaction between these partners or from their colocalization in specific microdomains.
- Data show that Gi and RGS proteins provide biochemical control of androgen receptor exclusion from the cell nucleus.
- Selective induction of G alpha inhibiting subunit 1 (Gi alpha1) expression is a novel downstream event in hypertrophic signaling that may be a critical factor leading to cellular electrophysiological remodeling of the Ras transgenic mouse heart.
- Heretotrimeric G protein subunit Galphai is associated with mitochondria.
- The potency and efficacy of LPA-mediated inhibition of forskolin-stimulated adenylyl cyclase activity was enhanced in cells expressing RGSi G(i) (mutant) proteins as compared to RGSwt G(i).
- MUPP1 binds to the G protein-coupled MT(1) melatonin receptor and directly regulates its G(i)-dependent signal transduction
- analysis of structural determinants underlying the temperature-sensitive nature of a Galpha mutant
- Galpha.GoLoco complexes have roles in mitotic spindle dynamics