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Validated All-in-One™ qPCR Primer for GLI1(NM_005269.2) Search again
Product ID:
HQP007701
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GLI, PAPA8, PPD1
Gene Description:
GLI family zinc finger 1
Target Gene Accession:
NM_005269.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation. [provided by RefSeq].
Gene References into function
- Cooperative E-box regulation by TWIST and USF
- Sonic hedgehog activates expression during prostate ductal bud formation
- Loss of protein kinase Calpha expression may enhance the tumorigenic potential of Gli1 in basal cell carcinoma.
- GLI-1 has a role in mediating bcl-2 transcriptional regulation by the sonic hedgehog signaling pathway
- CDK4, MDM2, SAS and GLI genes are amplified in leiomyosarcoma, alveolar and embryonal rhabdomyosarcoma
- GLI oncogene is activated through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms
- GLI2 directly activates GLI1 and retrovirally expressed GLI2 induces expression of endogenous GLI1 in human primary keratinocytes
- Data report expression of sonic hedgehog-GLI-1 pathway components in adult human prostate cancer, often with enhanced levels in tumors versus normal prostatic epithelia.
- characterization of the breakpoints of the ACTB-GLI and GLI-ACTB fusions at the genomic level in pericytoma with t(7;12)
- Quantification of Gli1 transcripts by RT-PCR is helpful in discriminating BCC and trichoepithelioma from other skin tumors.
- Elevated expression of hedgehog target genes human patched gene 1 (PTCH1) or Gli1 occurs in 63 of the 99 primary gastric cancers. (patched gene 1)
- downregulation of Gli1 expression may be an important mechanism by which KAAD-cyclopamine inhibits growth and induces apoptosis in esophageal cancer cells
- GLI1 induction was apparently inhibited by PTCH1
- Protein kinase A regulates Gli1 localization and its transcriptional activity, in part, through modulating the nuclear localization signal function.
- GLI target gene profiles correlated well with the biological activities of these transcription factors in hair follicles and basal cell carcinoma.
- Increased expression of SHh mRNA in human colonic adenocarcinomas and in a colorectal cell line with downstream increased expression of Gli1 mRNA known to promote cell proliferation.
- beta-catenin might be involved in the Hh signaling pathway via enhancement of the transcriptional activity of GLI
- The results suggest that epidermal growth factor receptor (EGFR) signaling modulates GLI target gene profiles which may play an important regulatory role in outer root sheath (ORS) specification, hair growth, and possibly cancer.
- AN11 may be a physiological regulator of GLI1 transcriptional activity
- Expressions of Shh and Gli-1 were significantly higher in leiomyomas than in gastrointestinal stromal tumors. Expressions of Ptc and Smo did not correlate with histopathological differentiation.
- SCCRO regulates Gli1--a key regulator of the hedgehog (HH) pathway.
- gastrointestinal neuroendocrine carcinoma cell lines express Gli1 mRNA significantly.
- Hedgehog transcription factor Gli1 is targeted by Numb for Itch-dependent ubiquitination, which suppresses Hedgehog signals, thus arresting growth and promoting cell differentiation.
- In esophageal squamous cell carcinoma, Gli-1 expression was associated with tumor depth (p < 0.001), positive lymph node metastasis (p = 0.004) and a poor prognosis (p = 0.0047).
- GLI signaling regulates the expression of stemness genes in and the self-renewal of CD133(+) glioma cancer stem cells.
- Melanomas require SHH-GLI signaling regulated by interactions between GLI1 and the RAS-MEK/AKT pathways.
- Shh-Ptch1-Gli1 signaling pathway may play a role in the progression of colorectal tumor.
- mRNA expression of the hedgehog pathway target Gli1 is relatively high in approximately 25% of glioblastoma multiforme cases. Reduction of Gli levels using siRNA also reduced the viability and growth of glioma cells.
- transforming growth factor-beta has a role in Smad3-dependent activation of Gli2 and Gli1 expression
- Down-regulation of Bcl-2 plays an important role in apoptosis induced by Gli-1 siRNA in hepatocellular carcinoma cells
- GLI1 does not routinely co-operate with ERK to induce the formation of basal cell carcinoma skin cancers.
- activation mechanism of the terminal transducer of the pathway, GLI1, is mediated not only by GLI1FL but also by the GLI1DeltaN variant
- members of the Hh pathway, especially Gli1, play an important role in the invasiveness of pancreatic cancer cells through the regulation of MMP-9 expression.
- SHH and Gli1 mRNAs are likely to be up-regulated from adenoma and from borderline to carcinoma cells, respectively, in intraductal papillary mucinous neoplasm of the pancreas
- Gli-1 nuclear expression is a strong and independent predictor of early relapse and poor prognosis in Esophageal Squamous Carcinoma after ChemoRadioTherapy - which suggests that Hh signal activation might promote cancer regrowth and progression after CRT
- These results indicate a significant role for Shh-GLI signaling in the proliferation of mantle cell lymphoma.
- Hh target genes GLI1 and PTCH1 are not expressed in lesional psoriatic skin
- A role ofor SIL in derepressing GLI1 from the negative control of SUFU.
- p53 and GLI1 may have a role in tumor cell survival
- Protein kinase Cdelta alters hedgehog signaling by inhibition of Gli protein transcriptional activity
- Reduced GLI1 function predisposes to a heightened myeloid response to inflammatory stimuli, potentially leading to IBD.