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Validated All-in-One™ qPCR Primer for LRIG1(NM_015541.2) Search again
Product ID:
HQP006969
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
LIG-1, LIG1
Gene Description:
leucine rich repeats and immunoglobulin like domains 1
Target Gene Accession:
NM_015541.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Down-regulation of LIG-1 is associated with cancer
- Results demonstrate that LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues and that its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments.
- downregulation of LRIG1 is associated with renal cell carcinoma
- LRIG1 evolved in mammals as a feedback negative attenuator of signaling by receptor tyrosine kinases
- LRIG1-mediated receptor ubiquitination and degradation may contribute to the suppression of ErbB receptor function
- A breast cancer linked gene is located within an amplicon containing the LRIG1 locus at 3p14.3.
- Lrig1 maintains epidermal stem cells in a quiescent nondividing state, and Lrig1 down-regulation triggers proliferation
- LRIG1 is a novel negative regulator of the Met receptor and opposes Met and Her2 synergy.
- a role of LRIG1 in tumor suppression in the uterine cervix
- Great heterogeneity in the expression of the LRIG1 protein in colorectal cancer, which was not related to gene dosage of the LRIG1 gene.
- LRIG proteins may have a role in epidermal homeostasis and psoriasis
- loss of LRIG1 in tumors may contribute to a permissive environment for EGFRvIII overexpression, contributing to EGFRvIII oncogenesis.
- Suppression of the negative regulator LRIG1 contributes to ErbB2 overexpression in breast cancer.