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Validated All-in-One™ qPCR Primer for AMBP(NM_001633.3) Search again
Product ID:
HQP006953
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Gene Description:
alpha-1-microglobulin/bikunin precursor
Target Gene Accession:
NM_001633.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq].
Gene References into function
- Transfection into H460M Human large cell lung carcinoma line decreased tumor growth and metastasis in nude mice
- Processing of the lipocalin alpha(1)-microglobulin by hemoglobin induces heme-binding and heme-degradation properties.
- bikunin has a role in downregulating PI3 kinase and suppressing tumor invasiveness and metastasis in ovaran cancer cells
- Data show that alpha(1) -microglobulin/bikunin precursor specifically interacts with the ORF3 protein of hepatitis E virus and has a direct role in enhancing alpha(1)microglobulin export from the hepatocyte.
- alpha-1-microglobulin is covalently bound to kynurenine-derived chromophores
- Bikunin neither decreased expression of TGF-beta receptors (TbetaRI and TbetaRII) in cancer cells nor altered the specific binding of 125I TGF-beta1 to the cells.
- The TSG-6 and inter-alpha-inhibitor interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link.
- the second processed protein from AMBP, bikunin, strongly interacted with the full-length hepatitis E virus ORF3 protein
- the ORF3 protein exploits the endosomal sorting machinery to enhance the secretion of an immunosuppressant molecule (alpha1 microglobulin) from cultured hepatocytes
- The urinary excretion of alpha1-microglobulin and albumin was significantly elevated in nine patients, and the urinary excretion of the two proteins correlated significantly.
- Identification of the protein bikunin as an endogenous, competitive inhibitor of a dynorphin-converting enzyme in human cerebrospinal fluid.
- Free uristatin and bikunin pass readily into urine and are primarily bound to heavy chains that constitute the proinhibitor form in plasma.
- Bikunin was found to localize on the cell membrane, while tryptase was in the secretary granules of the mast cells from psoriatic lesions.
- alpha(1)-microglobulin participates in the defense against oxidation by hemoglobin, heme, and reactive oxygen species
- small angle X-ray scanning study of the monomer and dimer forms of alpha1-microglobulin; monomer is characterised by a radius of gyration R(G)= 2.20 nm and D(max)=6.3 nm and the dimer by R(G)=2.99 nm and D(max)=9.5 nm
- The ability of alpha-1-microglobulin to restore the initial conformation characteristic for the native protein and the internal dynamics after the unfolding of the globule by 10 M urea and 6 M guanidine hydrochloride has been ascertained.
- AMBP has radical scavenging activity.
- urinary trypsin inhibitor is insufficiently produced for neutrophil elastase release after cardiac arrest
- in multiple myeloma patients without renal failure, elevated Alpha-1-microglobulin excretion might be an early marker of renal tubular involvement
- In the present study, structural motifs regulating bikunin's anti-inflammatory function are delineated. Binding of bikunin to neutrophils is critically important for its anti-inflammatory capacity.
- The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.
- Overexpression of bikunin reduced the gene expression of matriptase, which attenuated in vitro cell invasion in prostate cancer.