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Validated All-in-One™ qPCR Primer for NR5A2(NM_003822.4) Search again
Product ID:
HQP006437
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2
Gene Description:
nuclear receptor subfamily 5 group A member 2
Target Gene Accession:
NM_003822.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- role in regulating aromatase expression in preadipocytes (liver receptor homologue-1)
- Ligands are dispensabe for LRH-1 activation.
- LRH-1 is a nuclear receptor which can assume an active conformation in the absence of a ligand agonist.
- Liver receptor homolog 1 controls the expression of carboxyl ester lipase in pancreas
- PDX-1 regulates expression of LRH-1 during pancreas development.
- LRH-1 is highly expressed in corpus luteum, and it plays an essential role in the regulation of HSD3B2.
- hBIF and HNF1 are involved together in the viral gene expression regulation of the Hepatitis B virus.
- role for LRH-1 in the induction of the progesterone but not the estrogen biosynthetic pathway during granulosa cell differentiation.
- LRH-1 is a positive transcription factor for ABCG5 and ABCG8 and, in conjunction with studies on LRH-1 activation of other promoters, identify LRH-1 as a "master regulator" for genes involved in sterol and bile acid secretion from liver and intestine
- important functional role of helix 1 in cofactor recruitment and a novel molecular mechanism of transcriptional regulation and cofactor recruitment mediated by hLRH-1
- LRH-1 could be the major transcription factor responsible for the rapid and significant increase in ovarian StAR gene expression after ovulation.
- The liver receptor homolog-1 has emerged as an essential regulator for the expression of cyp7a1 gene.
- LHR-1 regulgates apolipoprotein A-1 transscription, and affects cholesterol homeostasis.
- CYP11A1 expression in human granulosa cells is regulated by LRH-1.
- Human LRH-1 receptor binds phosphatidyl inositol second messengers;ligand binding is required for maximal activity.
- hLRH-1's control of gene expression is mediated by phospholipid binding
- Data show that sumoylated LRH-1 is exclusively localized in promyelocytic leukemia protein nuclear bodies, and that this association is a dynamic process regulated in part by SUMO-1.
- suppression of hLRH-1 resulted in cell cycle arrest mediated by the down-regulation of cyclin E1
- LRH-1 is transcriptionally regulated by the estrogen receptor alpha reinforcing the hypothesis that LRH-1 could exert potential oncogenic effects in breast cancer formation.
- LRH-1 and SHP1 regulate 3-hydroxy-3-methylglutaryl coenzyme A reductase promoter and have a role in regulation of cholesterol synthesis and uptake
- phosphorylation of the hinge domain of the nuclear hormone receptor LRH-1 stimulates transactivation
- siRNA interference studies suggested that nuclear receptor subfamily 5, group A, member 2 (hLRH-1) acts as a negative regulator in farnesyl pyrophosphate synthetase expression.
- FTF and LRH-1 are two related but different transcription factors in human Caco-2 cells, suggesting that they may be homologues and not orthologues.
- LRH-1 and SF-1 have qualitatively similar actions on FSH-stimulated estrogen and progesterone production. These factors may have overlapping actions in regulation of steroidogenesis that accompanies granulosa cell differentiation.
- LRH-1 stimulation of the FAS LXR response is blocked by the addition of small heterodimer partner (SHP) and that FAS mRNA
- LRH-1/phospholipid and LRH-1/SHP (fragments) interactions are analyzed by counting atomic contact number, identifying hydrogen bonds, and estimating binding free energies
- The present study demonstrated for the first time the increased expression of hLRH-1v1 and hLRH-1 in human gastric cancer, an alteration which may implicate in tumorigenesis.
- LRH-1 is a novel regulator of APOM transcription and further extend the role of this orphan nuclear receptor in lipoprotein metabolism and cholesterol homeostasis
- Sphingosine-1-phosphate induces LRH-1 mRNA expression in MCF-7 cells in a prostaglandin E2 (PGE2)-dependent manner.
- Liver receptor homolog 1 (LRH-1) is a key transcriptional factor required for the hepatic expression of CYP7A1.
- PGC-1alpha is an important co-activator for LRH-1 and that SHP targets the interaction between LRH-1 and PGC-1alpha to inhibit CYP7A1 expression.
- a study, by molecular dynamics (MD) simulations, the impact of the ligand on the receptor and the interaction with different cofactor peptides
- Possible unexpected new class of nuclear receptor signaling molecules, but broader functional roles of LRH-1 and these new ligands remain to be established.[REVIEW]
- Differential expression of steroidogenic factors 1 and 2, cytochrome p450scc, and steroidogenic acute regulatory protein in the pancreas.
- LRH-1 is aberrantly expressed on solid tumor cells
- structure of the Dax-1:LRH-1 complex provides the molecular mechanism for the function of Dax-1 as a potent transcriptional repressor
- both SF1 and LRH1 can transcriptionally cooperate with the AP-1 family members c-JUN and c-FOS, known to be associated with enhanced proliferation of endometrial carcinoma cells, to further enhance activation of the STAR, HSD3B2, and CYP19A1 PII promoter