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Validated All-in-One™ qPCR Primer for CADM1(NM_014333.3) Search again
Product ID:
HQP006321
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BL2, IGSF4, IGSF4A, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1
Gene Description:
cell adhesion molecule 1
Target Gene Accession:
NM_014333.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- TSLC1 mediates intracellular adhesion through homophilic interactions in a Ca(2+)/Mg(2+)-independent manner
- alteration of TSLC1 is involved in prostate cancer
- The TSLC1 promoter region has many methylated cytosines in the CpG islands in chromosome 11q LOH in breast cancer.
- SynCAM1 is expressed in brain and localized to pre- and postsynaptic sites of neurons. SynCAM1 is a homophilic cell adhesion molecule that induces formation of presynaptic terminals by neurons.
- methylation and subsequent inactivation of TSLC1 expression is associated with pancreatic adenocarcinoma
- bi-allelic hypermethylation of the TSLC1 promoter and resulting gene silencing occur in a subset of primary gastric cancers
- The results support the hypothesis that TSLC1 is a tumor suppressor of NSCLC and also suggest that preserved integrity of TSLC1 may contribute to less invasive phenotypes of lepidic growth tumor cells.
- alteration of TSLC1 expression by romoter methylation is involved in advanced nonsmall cell lung cancer
- TSLC1 represents central effector gene for controlling biological aggressiveness of pulmonary adenocarcinoma and is essential biomarker for predicting prognosis
- IGSF4 was found to have roles in adhesion of spermatogenic cells to Sertoli cells and mast cells to fibroblasts and synaptic formation of neural cells--REVIEW
- Lung tumor suppressor gene,TSLC1, associates with MPP3, a human homologue of Drosophila tumor suppressor Dlg.
- The loss of TSLC1 expression has an important role in tumor growth, cell motility, and invasion and is associated with aggressive tumor behavior in esophageal squamous cell carcinoma.
- A549 lung cancer cells expressing wild-type TSLC1 showed suppression of anchorage-independent colony formation in soft agar and markedly increased cell-cell adhesion activity.
- Epigenetic inactivation of TSLC1 gene is associated with nasopharyngeal carcinoma
- TSLC1 gene silencing via promoter hypermethylation is a frequent event in the progression from high-risk HPV-containing, high-grade CIN lesions to invasive cervical cancer
- Our results demonstrate the pro-apoptotic and oncosuppressive activity of TSLC1 protein.
- Ectopic expression of TSLC1 could provide a novel marker for acute-type adi;t acite t-cell leukemia and may participate in tissue invasion, a characteristic feature of the malignant ATL cells.
- TSLC1 plays an important role in meningioma pathogenesis.
- Necl2/CRTAM molecular pair could regulate a large panel of cell/cell interactions both within and outside of the immune system
- NK cells and T8 cells recognize Necl-2 through CRTAM, expressed only on activated cells. CRTAM-Necl-2 interactions promote cytotoxicity of NK cells and IFN-gamma secretion of T8 cells as well as NK cell-mediated rejection of tumors expressing Necl-2
- SgIGSF/SynCAM expressed on transgenic mouse mast cells and superior cervical ganglion neurons appears to predominantly mediate attachment and promote communication with nerves.
- Gene expression and cell cycle differences provide insights into potential downstream pathways of TSLC1 that mediate the suppression of tumor properties in A549 cells
- Loss of TSLC1 is associated with lower patient survival, supporting its role as a tumor suppressor in lung adenocarcinoma.
- methylation of TSLC1 leading to loss of the expression, is an important event in the pathogenesis of non-small-cell lung cancer
- SynCAM proteins are encoded by a family of four conserved IGSF4 genes found solely in vertebrates; transcripts can be alternatively spliced and encode proteins with three immunoglobulin-like domains.
- lung mast cells adhere avidly to airway smooth muscle in part via TSLC-1 and in part via an as-yet-undefined Ca2+-dependent pathway
- TSLC1 inhibits nasopharyngeal carcinoma cell growth by arresting cells in G(0)-G(1) phase in normal culture conditions. Without serum, TSLC1 induced apoptosis. TSLC1 is a tumor suppressor gene in NPC; its loss is seen in lymphatic metastasis.
- TSLC1 protein and RNA expression is lost in 60% to 65% of high-grade gliomas, and TSLC1 reintroduction into glioma cells results in growth suppression.
- TSLC1 and DAL-1 are involved in the pathogenesis of breast cancer and are frequently inactivated by methylation
- These results indicate that TSLC1 is a novel interameloblast adhesion molecule that may be downregulated during ameloblastic tumorigenesis.
- We propose that SgIGSF is a novel and functional biliary epithelial cell adhesion molecule that is expressed for a limited time during active bile duct/ductule formation [SgIGSF].
- Downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression and unfavourable patient outcome.
- Appears to mediate efficient adhesion and growth of malignant pleural mesothelioma cells specifically on mesothelial cells, probably via trans-heterophilic binding.
- The strong correlation between CADM1 expression and hormonally functional phenotypes suggests that CADM1 is involved in hormone secretion from ICTs.
- Density of promoter methylation was associated with the degree of anchorage-independent growth and CADM1 gene silencing in vitro. In cervical squamous lesions, methylation frequency and density increased with severity of disease
- study puts CADM1 forward as a strong candidate neuroblastoma suppressor gene
- Airway smooth muscle cells maintain human lung mast cell survival in vitro by a direct interaction between the two cell types that leads to rapid cell proliferation through a cooperative CADM1-, stem cell factor, and interleukin 6-dependent mechanism.
- TSLC1 acts as a candidate tumor suppressor gene for neuroblastoma.
- TSLC1 expression in Adult T-cell leukemia (ATL) cells plays an important role in the growth and organ infiltration of ATL cells.
- Two missense mutations, C739A(H246N) and A755C(Y251S), in the CADM1 gene of male Caucasian autism spectrum disorder patients and their family members were found.
- Our results demonstrate a frequent, predominant epigenetic silencing of CADM1 and DAL-1 in nasal NK/T-cell lymphoma (NL), which likely play a synergic role in NL pathogenesis.