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Validated All-in-One™ qPCR Primer for FLT1(NM_002019.3) Search again
Product ID:
HQP005879
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FLT, FLT-1, VEGFR-1, VEGFR1
Gene Description:
fms related receptor tyrosine kinase 1
Target Gene Accession:
NM_002019.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM].
Gene References into function
- vitronectin increased the presence of all four growth factor receptors and most notably, VEGFR-1; in contrast, fibrin decreased all four receptors, especially FGFR-1 and FGFR-2
- presence of an sVEGFR-1 in human serum and plasma of normal male and female donors strongly suggests that it plays an important role as a naturally occurring VEGF antagonist in the regulation and availability of VEGF-mediated biological activities in viv
- A potential mechanism involved in hemangioma formation is the alteration of the FLT1 receptor signaling pathway in endothelial and/or pericytic cells.
- Effect of placenta growth factor-1 on proliferation and release of nitric oxide, cyclic AMP and cyclic GMP in human epithelial cells expressing the FLT-1 receptor
- Involvement of VEGFR-2 (kdr/flk-1) but not VEGFR-1 (flt-1) in VEGF-A and VEGF-C-induced tube formation by human microvascular endothelial cells in fibrin matrices in vitro.
- VEGF and flt-1 are upregulated in blood vessels in many organs of acute Kawasaki Disease
- In this study we give evidence of Flt-1 and KDR receptors in platelets.
- expression, purification and detection of biological activity
- CLL B cells consistently express VEGFR1 mRNA; co-expression of angiogenic molecules and receptors suggest autocrine pathways of stimulation.
- Placental growth factor promotes recruitment of VEGFR1(+) hematopoietic stem cells from a quiescent to a proliferative bone marrow microenvironment, favoring differentiation, mobilization and reconstitution of hematopoiesis.
- Expression of vascular endothelial growth factor and its receptor (Flt-1) in breast carcinoma
- results suggest that in the hematopoietic microenvironment an autocrine vascular endothelial growth factor loop contributes to optimal megakaryocytic maturation through Flt1
- In human umbilical vein endothelial cells, the Flt-1 receptor appears to act as a decoy receptor, tempering the response to lower concentrations of VEGF.
- The expression of vascular endothelial growth factor and its receptors KDR and Flt-1 by gastric carcinoma tissues and cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth.
- This protein is a novel therapeutic target for angiogenic disorders (REVIEW)
- Staining for the receptors VEGFR-1 and VEGFR-2 was positive in large lymphoid cells in stage IV non-Hodgkin lymphoma.
- expression system is involved in angiogenesis in inflamed synovial tissue in the temporomandibular joint
- VEGF secreted by retinal pigment epithelial cells upregulates pigment epithelium-derived factor expression via VEGFR-1 in an autocrine manner.
- findings may point to an involvement of soluble vascular endothelial growth factor receptor-1 in the pathophysiology of preeclampsia possibly by antagonizing vascular endothelial growth factor effects
- Aberrant methylation of the vascular endothelial growth factor receptor-1 gene is associated with prostate cancer
- VEGFR-1 secreted by endothelial cells becomes a matrix-associated protein that is able to interact with the alpha 5 beta 1 integrin; a new role of VEGFR-1 in angiogenesis
- in humans: 1) VEGF, KDR, and Flt-1 mRNA are increased by acute systemic exercise; 2) the time course of the VEGF, KDR, and Flt-1 mRNA responses are different from those previously reported in rats
- Postmortem brain tissue analysis demonstrates VEGFR1 localized as paracellular deposits in Durck's granulomas of patients with cerebral malaria.
- mRNA expression of VEGF and its receptor flt-1 in the hydrosalpinx was significantly higher than that in the healthy oviduct.
- overproduction of soluble VEGFR-1 may lead to suppression of VEGF-A and PlGF and the down-regulation of its membrane bound form (VEGFR-1) in the placental bed, may result in the defective uteroplacental development.
- Data suggest that vascular endothelial growth factor (VEGF) receptor flt-1 is expressed by eosinophils whose activation with VEGF stimulates directed migration and release of eosinophil cationic protein.
- VEGF, VEGFR-1 and VEGFR-2 are concomitantly expressed in pre-B ALL cells. Expression of the receptors is limited to the intra-cytoplasmic compartment and may suggest either internalization or a block in trafficking of the receptor to the surface.
- changing of transcriptional activity of VEGF gene and its receptor FLT-1 indicates an autocrine mechanism of regulation of angiogenic gene activity in the first step of carcinogenesis--low-grade intraepithelial lesions of the uterine cervix
- crystal structure of placental growth factor in complex with domain 2 of vascular endothelial growth factor receptor-1
- Specific VEGFR1 expression, examined in 27 B-CLL samples, was positive in all of them. The VEGF transduction pathway may be very active in CLL cells. Both its paracrine & autocrine pathways may contribute to their enhanced survival.
- in systemic lupus erythematosus patients the levels of VEGF and sVEGFR-1 are higher in patients with active SLE than in inactive disease or healthy persons
- flt-1 appears to be important in the temporal regulation of oviductal secretion.
- VEGF receotor signaling regulates survival signals in CLL cells and that interruption of this autocrine pathway results in caspase activation and subsequent leukemic cell death.
- C-Myc over-expression was significantly associated with high sVEGF and normal sFlt-1 level in DLBCL patients, suggesting a complex interrelationship between c-Myc oncogene expression and angiogenic regulators
- Increased expression of FLT1 is associated with an aggressive angiogenic phenotype in melanoma.
- VEGFR1 initiates a clonogenic response in myeloid leukemia cells that is PI3-kinase dependent.
- a basis for understanding molecular recognition between PlGF-1 and VEGFR1
- cytotrophobasts possess a unique property to enhance sFlt-1 production under reduced oxygen
- Preeclamptic placental villous explants showed a four-fold increase in sVEGFR-1 over normal pregnancies. Elevated levels of sVEGFR-1 in preeclampsia are responsible for inhibiting angiogenesis.
- There was a significant positive correlation between the level of expression of VEGF and VEGF-R1 (P = 0.04) in both control groups and lung bearing tumorlets.
- During tumour progression there is a change in the relative amounts of sFIt-1 and vascular endothelial growth factor in the circulation.
- Granulocyte-Macrophage Colony-Stimulating Factor and monocytes play a vital role in angiogenesis through the regulation of VEGF and sVEGFR-1
- VEGF and sFLT-1 can actively take part in the pathogenesis of diabetic nephropathy
- These data support the involvement in melanoma growth and survival of a VEGF-dependent internal autocrine loop mechanism, at least in vitro.
- Subjects with acute mountain sickness have lower levels of soluble VEGF receptor (Flt-1) at both low and high altitude compared with well subjects.
- Blocking VEGF and PDGF receptor signaling in cardiac allografts has distinctive effects on inflammation and survival.
- in vivo EPO does not affect the functionality and/or production of components of the VEGF/Flt-1 system in diabetics with normal or reduced renal function
- Relative to human VEGF165, the binding affinity of Pm venom VEGF to the human VEGFR-1 was 1.7-fold higher while affinity to the VEGFR-2 was 17-fold lower. But it did not bind the VEGFR-3 or neuropilin-1.
- fms-like tyrosine kinase 1, a circulating antiangiogenic protein, may play an important role in the pathogenesis of preeclampsia [review]
- When used individually, FGFR1 partially prevented goiter and sVEGFR1 partially reduced vascular volume.
- role for sFlt-1 in the maternal manifestations of preeclampsia
- These findings are consistent with the idea that the chemotactic effect of VEGF-A on mesenchymal progenitor cells (MPC) is mediated via VEGFR-1, and that VEGF-A and PlGF-1, have a functional role for recruitment of osteoprogenitor cells.
- Increased sFlt-1 secretion in first versus second pregnancies may account in part for the increased risk of preeclampsia among nulliparous women.
- In vitro stimulation of blood samples with bacteria-derived antigens resulted in a significant increase in soluble VEGF (p < 0.0001) and a less pronounced but still significant increase in soluble VEGFR1
- Up-regulation of VEGF-A receptor VEGFR-1 in capillaries in menorrhagia could be involved in abnormal endometrial vascular structure and permeability.
- We provide strong support that follicular fluid and granulosa cells control VEGF availability by down regulation of the soluble antagonist sFlt-1 leading to an increase of free, bioactive VEGF for maximal induction of vessel growth in the ovary.
- FLT-1 targeting on subsets of acute leukemias may delay the onset of extramedullary disease, which may be advantageous in combinatorial therapeutic settings.
- expression of VEGF receptor-1 in normal human epidermal keratinocytes; a role for VEGFR-1 in the proliferation of keratinocytes was found
- the conserved aspartic acid in the activation loop favors the transphosphorylation of the activation loop tyrosines of VEGFR1, and its absence renders RTK to a less potent enzyme by disfavoring transphosphorylation of activation loop tyrosines
- findings demonstrate a requirement for VEGFR1+ haematopoietic progenitors in the regulation of metastasis, and suggest that expression patterns of fibronectin and VEGFR1+VLA-4+ clusters dictate organ-specific tumour spread
- the VEGF system is integrated into the p53 transcriptional network by an SNP in the flt-1 promoter
- soluble vascular endothelial growth factor receptor-1 may have a role in onset of early and late ovarian hyperstimulation syndrome
- High mFlt-1/VEGF tumor showed a significantly lower microvessel density and patients with high mFlt-1/VEGF tumor had a significantly favorable survival.
- VEGF receptors may not only modulate angiogenesis, but also directly influence the growth of VEGF receptor expressing tumors
- VEGFR-1 promotes migration of tumour cells through a Src-dependent pathway linked to activation of focal adhesion components that regulate this process in colonic adenocarcinoma.
- VEGFR-1 and VEGFR-2 have roles in differentiation of hepatocellular carcinoma
- overexpression of VEGF, its receptors flt-1, KDR/flk-1 and TGF-beta interaction may play an important role in the ovarian cancer biology, with potential effects on tumor growth and angiogenesis
- multiple myeloma cells (but not B-cell chronic lymphocytic leukemia cells) induced a dramatic increase in expression of VEGFR-1 and VEGFR-3 on human bone marrow endothelial cells
- Reduced expression of VEGF and VEGFR-1 in lung tissue may contribute to the death of alveolar epithelial cells.
- VEGF regulation of angiogenesis may in part be due to enhanced proliferation of VEGFR-1 and VEGFR-2
- modulation of the ratio of VEGF and sFlt-1 in the fallopian tube may contribute to the normal and pathological processes of oviductal fluid secretion by regulating oviductal vascular permeability during the menstrual cycle
- These results suggest that the expression of VEGFRs and NRPs on keratinocytes may constitute important regulators for its activity and may possibly be responsible for the autocrine signaling in the epidermis.
- This review highlights the roles of VEGFR-1 as a negative regulator for angiogenesis at embryogenesis and as a positive regulator in adulthood, making it an important target in the development of new strategies to suppress disease.
- The maternal plasma levels of VEGFR1 in patients with mirror syndrome (Ballantyne's syndrome) and in controls matched for gestational age are reported.
- Third-trimester increases in sFlt-1 and decreases in placental growth factor levels are associated with preeclampsia.
- Preeclampic placentas overexpressed sFlt mRNA as well as Eng protein.
- identify a second regulatory sequence comprising a partial response element for estrogen receptors upstream of the p53 binding site
- Women with preeclampsia and preexisting diabetes hve elevated blood levels of FLT1.
- The present findings suggest that IL-13 is a regulatory factor of VEGF and sFlt-1 production in the human fallopian tubes.
- Endometrial blood vessels possess a discrete morphology that is characterized by endothelial gaps, and these gaps are more pronounced in women with menorrhagia and overexpression of VEGFR1.
- THPO and VEGF play an important synergistic role in the formation of early ES-derived hematopoietic progenitors that occurs through the c-mpl and VEGF receptors.
- The serum sFlt-1/Ang-2 ratio is significantly elevated in preeclamptic women as compared to healthy pregnant women.
- Vascular endothelial growth factor receptor-1 expressing cells in the peripheral blood were more abundant in cancer cases with metastases than in cases without metastases.
- VEGFR1 is not increased in normotensive intrauterine growth restriction placentas
- Results show a unique survival system in breast cancer cells by which VEGF can act as an internal autocrine (intracrine) survival factor through its binding to VEGFR1, and may lead to a strategy for tumor therapy based on the inhibition of angiogenesis.
- High VEGFR-1 expression is associated with intracranial schwannomas
- determined the expression pattern of VEGF splice variants in NSCLC and its correlation with the clinicopathological characteristics of tumors
- Cleavage of three distinct Flt1 intronic poly(A) signal sequences within intron 13 leads to an unusually large 3' untranslated region containing several potential AU rich elements and poly(U) regions that may regulate mRNA stability in the placenta.
- Prechemotherapy soluble VEGFR-1 can be helpful for prediction of long-term response to therapy in lung cancer.
- PGF and VEGFR1 may have an important role in the pathogenesis of the neovascular response in cirrhosis.
- Preeclampsia trophoblast cells produce more sEng, sFlt-1, and PlGF than normal TCs. Lowered oxygen conditions promote sEng and sFlt-1, but reduce PlGF, productions by PE TCs.
- Reduced placental perfusion may contribute to the increased expression of sFlt-1 in IUGR pregnancies. Data are compatible with differential sFlt-1 expression in placentas from discordant twins.
- G-CSF treatment induced reduced expression of CXCR4, VLA-4 and VEGFR-1 in patients with liver failure.
- lineage-negative, CD34-negative (Lin(-)CD34(-)) multipotent mesenchymal stromal cells express vascular endothelial growth factor receptor-1 (VEGFR-1), which mediates their response to vascular endothelial growth factor A
- sFlt-1 and PlGF and their ratio relative to one another may play a role in the development of preeclampsia
- lysine 831 of vascular endothelial growth factor receptor 1 is a novel target of methylation by SMYD3
- our data indicate that an excess of local VEGF with respect to its soluble receptor VEGFR-1 may be a key factor in the onset and maintenance of pathological neo-angiogenesis in ovarian cyst formation
- Results showed that flt-1 plays an important role in the development of retinal neovascular membranes but the role is uncertain in the iris and retina.
- Both PlGF-1 (placenta growth factor) and VEGF-A (vascular endothelial growth factor-A ) can activate VEGFR-1-(vascular endothelial growth factor receptor-1)dependent signaling pathways in primary human monocytes
- VEGFR-1 expression is not associated with the risk of cancer progression following RP. Therefore, VEGFR-1 may not be of prognostic value in clinically-localized PCa.
- patients destined to develop Preeclampsia had a significantly higher plasma concentration of sVEGFR-1 (vascular endothelial growth factor receptor) at 26 and 29 weeks of gestation
- Mothers with small for gestational age fetuses had high plasma vascular endothelial growth factor receptor(sVEGFR-1), but lower than preeclampsia patients; those with abnormal uterine and umbilical artery velocimetry had the highest sVEGFR-1
- PlGF and sFlt-1 are present in the maternal and fetal fluid compartments in very early pregnancy, and their distribution is consistent with their site of production and the local conditions of transport
- VEGF-A induction of VEGFR2-negative human aortic smooth muscle cell migration is mediated through a molecular cross-talk of VEGFR1 (Flt-1), neuropilin-1 (NRP-1), and phosphoinositide 3-kinase (PI3K)/Akt signaling kinase.
- FLT-1 mRNA levels were uniformly increased in congenital hemangiomas compared with proliferating or involuting phase common hemangioma.
- Expression of vascular endothelial growth factor-A and its receptor-1 in a luteal endometrium in patients with repeated in vitro fertilization failure.
- Surveillance of VEGFR-1 levels to predict chemotherapy response is not useful.
- an increased expression of fms-related tyrosine kinase 1 (FLT1), was detected in the placentas of patients with both preeclampsia and small for gestational age infants
- during CDDP-induced tumor progression the activation of VEGF/Flt1 autocrine signaling leads to the survival and expansion of a highly tumorigenic side-population fraction
- LH induces ovarian follicular angiogenesis via modulation of VEGF-A and its soluble receptor sFlt-1 expression.
- Found in normal and neoplastic thymus tissue.
- Seven placental transcripts characterize HELLP-syndrome.
- Bone marrow-derived cells expressing this gene can be mobilized into and cluster at distant sites, "the premetastatic niche"
- VEGF is considered as an important factor in the pathogenesis of macular edema. VEGF induces the rupture of the blood retinal barrier and may also influence the retinal pigment epithelial (RPE) outer retinal barrier.
- Elevated expression of VEGFR-1 facilitates the establishment of hematogenous metastases in gastric cancer. The simultaneous presence of isolated tumor cells and VEGFR-1 expression at premetastatic sites is clinically significant for disease progression.
- A soluble human-specific splicing variant of Flt1 in vascular smooth muscle cells, especially in placental cells in pre-eclampsia.
- Restoration of immunocompetence in metastatic renal cell carcinoma patients by pharmacological elimination of VEGFR1+ cells may have a significant impact on the therapeutic efficacy of cancer vaccines or other immune-based therapies
- Cigarette smoking is associated with lower maternal sFlt-1 concentrations during pregnancy and preeclampsia
- Initial and 24-h sFLT levels correlated with Acute Physiology Age Chronic Health Evaluation II and Sepsis-related Organ/Failure Assessment scores initially and at 24-h.
- High expression of ERCC1, FLT1, NME4 and PCNA in myelodysplastic syndrome was associated with poor prognosis and disease progression along the sequence of 5q-syndrome/RCMD/RAEB/de novo AML
- Serum levels of VEGF-A and its receptor, VEGFR-1, were significantly higher in patients with gastric cancer than in healthy donors.
- an angiogenic signal-ETS1/HIF-2alpha axis regulates the VEGFR1 chromatin domain to induce VEGFR1 transcription in endothelial cells and in differentiating embryonic stem cells
- Lysophosphatidic acid might regulate VEGF-C and lymphatic marker expression in endothelial cells, which contributes to endothelial cell tube formation in vitro and in vivo, thus facilitating endothelial cell participation lymphangiogenesis.
- d(TG)n repeat polymorphism of the Flt-1 gene is not associated with the development of preeclampsia in Korean pregnant women
- This is the first report demonstrating the inhibitory and stimulatory effects of IFN-gamma on VEGF-A and sFlt-1 secretion, respectively.
- VEGF and sVEGF-R1 were increased respectively to the degree of liver insufficiency in patients with liver cirrholsis.
- Cox regression analysis showed that the VEGF/sVEGFR-1 ratio was an independent predictor for pancreatic cancer survival. Higher VEGF/sVEGFR-1 ratio was significantly correlated with poor outcome in patents with pancreatic cancer.
- terms of serum levels of either VEGF or its soluble receptors VEGFR1, we found no significant difference between healthy individuals and women with benign breast tumors and breast cancer.
- Vascular endothelial growth factor receptor-1 contributes to anti-epidermal growth factor receptor drug resistance in different human cancer cells
- Soluble fms-like tyrosine kinase-1 is increased in the first trimester in women with subsequent late-onset preeclampsia and might therefore be useful to predict preeclampsia.
- FLT1 is under natural selection in a malaria endemic area and that human exposure to malaria can influence the evolutionary genetics of the maternal-fetal relationship
- The soluble Flt-1 level may be a suitable marker of disease severity and mortality in patients with septic shock
- VEGFR-1/-3 co-expression is associated with high hemoglobin level in childhood acute lymphoblastic leukemia
- Normalization of the constitutive VEGFR2 signaling in hemangioma endothelial cells with soluble VEGFR1 or antibodies that neutralize VEGF or stimulate beta1 integrin
- Demonstrates the utility of sVEGFR-1 as a sensitive and specific biomarker for the early onset (< or =34 weeks) of pre-eclampsia.
- Stimulation of the EP3(I) isoform of the human EP3 receptor with prostaglandin E(2) increases the mRNA expression of both VEGF and its cognate receptor VEGF receptor-1.
- Apoptotic levels do not correlate with Flt1 in preeclampsia placentae and are not regulated by in vivo exposure to the antihypertensives clonidine and hydralazine.
- glioma cells enhance EPC angiogenesis via VEGFR-2, not VEGFR-1, mediated by the MMP-9, Akt and ERK signal pathways
- sFlt-1 and sEng may be important in the regulation of angiogenesis in SCD.
- Autoantibodies against angiotensin II type 1 receptor (AT1-AAs) were common in patients with preeclampsia, although sFlt-1 was a superior biomarker.
- Under hypoxia enhanced levels of VEGFR1 on human umbilical vein endothelial cells were observed, while in human colonic microvascular endothelial cells there was a variable reaction to hypoxia, with cases of enhanced, unchanged and reduced expression.
- Smoking does not affect circulating maternal sVEGFR-1 concentrations or placental secretion of sVEGFR-1 or expression of VEGFR-1 in vitro.
- VEGFR1 receptor tyrosine kinase localization to the Golgi apparatus is calcium-dependent.