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Validated All-in-One™ qPCR Primer for MAPRE1(NM_012325.2) Search again
Product ID:
HQP005590
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EB1
Gene Description:
microtubule associated protein RP/EB family member 1
Target Gene Accession:
NM_012325.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells.
Gene References into function
- EB1 employs the hydrophobic interaction to bind to microtubules
- neither APC nor p150glued binding domain is necessary for EB1 or EBF3 to induce microtubule bundling
- APC and EB1 together give rise to similar defects in mitotic spindles and chromosome alignment without arresting cells in mitosis.
- MCAK is held in an inactive conformation when associated with EB1
- Aurora-B is an EB1-interacting protein; EB1 stimulates Aurora-B activity through antagonizing its dephosphorylation/inactivation by PP2A
- Proteomic profiling revealed the molecular signature behind the progression of hepatocellular carcinoma (HCC), and the prognostic value of EB1 in HCC.
- EB1 specifically recognizes the distinct lattice structure at the growing microtubule end; EB1 is both necessary and sufficient to mediate plus-end tracking by CLIP-170.