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Validated All-in-One™ qPCR Primer for FGF8(NM_006119.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells.
Gene References into function
- widespread expression pattern suggests FGF8 has physiological roles in adult tissue as well as in development
- Promoter of FGF8 reveals a unique regulation by unliganded RARalpha.
- Regulation of FGF8 expression by the androgen receptor in human prostate cancer.
- isoform b isoform expressed in prostate cancer, and is of prognostic value
- Repression of fgf8 might be directly or indirectly involved in this transcriptional control by TGF-beta.
- We also show that the mode of FGF8 receptor-binding specificity is distinct from that of other FGFs and provide the first biochemical evidence for a physiological FGF8b-FGFR1c interaction during mid-hindbrain development.
- novel role for nuclear factor-kappaB in the regulation of fibroblast growth factor 8(FGF8) expression in prostate cancer cells is uncovered
- These results indicate that mutations are rare in FGF8 and FGFR2 in hypospadias, but gene variants may influence the risk.
- FGF8 has a role in regulation of autocrine and paracrine loops in the growth regulation of breast, prostate and ovarian cancer [review]
- Fgf8 is required for the node to impart left-right asymmetry on specific tissues Excess Fgf8 signaling following a loss of RA may stimulate the node to generate asymmetry in presomitic mesoderm, leading to left-right asymmetry in somitogenesis clock
- FGF-8 is expressed at a high frequency in bone metastases of human prostate cancer and expression of FGF-8 in PC-3 prostate cancer cells increases their growth as intratibial tumors.
- Decreased FGF8 signaling causes deficiency of GnRH in humans.