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Validated All-in-One™ qPCR Primer for FCGRT(NM_004107.4) Search again
Product ID:
HQP005323
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FCRN, FcgammaRn, alpha-chain
Gene Description:
Fc gamma receptor and transporter
Target Gene Accession:
NM_004107.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Human FcRn binds selectively to human, rabbit and guinea pig IgG but not significantly to rat, bovine, sheep or mouse IgG (except for weak binding to mouse IgG2b).
- Assembly of the FcRn alpha-chain with beta(2)microglobulin is important for both transport of FcRn from the ER to the cell surface and efficient pH-dependent IgG binding.
- Functional reconstitution in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin
- although a secreted soluble form of human FcRn does not dimerize, the membrane-anchored receptor can form both non-covalent and covalent dimers; dimerization of human FcRn occurs in the absence of its ligand, IgG
- Expression of FcRn is demonstrated along the human fetal intestine and in a human nonmalignant fetal intestinal epithelial cell line (H4), which by location indicates that FcRn could play a role in the uptake and transport of IgG in the human fetus.
- data show that it is possible to confer binding of mouse immunoglobulin G on human FcRn by mutagenesis of selected residues; observations are of direct relevance to understanding the molecular nature of the human FcRn-IgG interaction
- Analysis of the dynamics and properties of trafficking of FcRn in live microvascular endothelial cells shows that the primary site at which FcRn sorts IgGs for either salvage or lysosomal degradation is the sorting endosome.
- strong cell surface polarity displayed by hFcRn results from dominant basolateral sorting by motifs in the cytoplasmic tail that nonetheless allows for a cycle of bidirectional transcytosis
- FCRN is involved in IgG exocytosis.
- residues encompassing and extending away from the interaction site on the alpha2 helix of FcRn play a significant and most likely indirect role in FcRn-IgG interactions
- expression of a functional FcRn in normal human epidermal keratinocytes.
- FcRn leaves sorting endosomes in Rab4(+)Rab11(+) or Rab11(+) compartments
- These transport and localization data are in accordance with efficient hFcRn-mediated apical IgG recycling and basolateral directed IgG transcytosis in placental trophoblasts.
- FcRn binds IgG and albumin, salvages both from a degradative fate, and maintains their physiologic concentrations
- a variable number of tandem repeats promoter polymorphism influences the expression of the FcRn receptor, leading to different IgG-binding capacities
- FcRn fulfills a major role in IgG-mediated phagocytosis
- FcRn-mediated recycling is a major contributor to the high endogenous concentrations of IgG and serum albumin, two important plasma proteins.
- Our results show clear evidence that the conserved H166 is a key player in the FcRn-albumin interaction.
- Elucidation of intracellular recycling pathways leading to exocytosis of FCRN was facilitated by using multifocal plane micoscopy.
- Neonatal Fc receptor (FcRn) was expressed in various cells of the human skin (including keratinocytes, melanocytes, and histiocytes).
- This review summarizes FcRn biology.
- These data provide the first evidence that NF-kappaB signaling via intronic sequences regulates FcRn expression and function.
- These results suggest a novel mechanism for regulation of IgG transport by calmodulin-dependent sorting of FcRn and its cargo away from a degradative pathway and into a bidirectional transcytotic route.
- JAK/STAT-1 signaling pathway was necessary and sufficient to mediate the down-regulation of FcRn gene expression by IFN-gamma
- A previously undescribed role for FcRn in mediating the presentation of antigens by dendritic cells when antigens are present as a complex with antibody, is shown.
- The impact of two free cysteine residues (C48 and C251) of the FcRn heavy chain on the overall structure and function of soluble human FcRn is explored.
- Intracellular trafficking of FcRn is regulated by its intrinsic sorting information and/or an interaction with major histocompatibility (MHC) class II invariant chain (Ii).
- Results indicate that FcRn-dependent internalization of IgG may be important not only in cells taking up IgG from an extracellular acidic space, but also in endothelial cells participating in homeostatic regulation of circulating IgG levels.