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Validated All-in-One™ qPCR Primer for FCGR2B(NM_004001.4) Search again
Product ID:
HQP005293
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD32, CD32B, FCG2, FCGR2, FCGR2C, FcGRIIB, FcRII-c, FcgammaRIIb, IGFR2
Gene Description:
Fc gamma receptor IIb
Target Gene Accession:
NM_004001.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- The inhibitory receptor, Fc gamma RIIb2, is expressed on circulating human monocytes. In human primary monocytes Fc gamma RIIb2 expression is increased by IL-4, a Th2 cytokine and decreased by IFN-gamma, a Th1 cytokine.
- Although there is no phenotypic difference in Fc gamma RII expression (Fc gamma RIIb predominating on both adult and cord B cells), the inhibitory Fc gamma RIIb is expressed at lower levels on cord cells.
- Here we demonstrate for the first time FcgammaRIIb protein expression and function in human monocytes
- association of a new polymorphism of FCGR2B (I232T) with susceptibility to SLE in the Japanese.
- human malignant melanoma cells express the inhibitory low-affinity Fc(gamma) receptor Fc(gamma)RIIB1 in 40% of tested metastases
- hypothesized that inhibitory signaling through FcgammaRIIb would be counter-productive in germinal center cells undergoing selection by affinity maturation
- Fcgamma receptor IIb polymorphisms are associated with susceptibility to systemic lupus erythematosus in Thais
- The differential activity of FCGR2B alleles suggests a novel mechanism of FcgammaRIIb regulation that may influence the risk of autoimmune disease.
- FCGR2B is a common susceptibility factor to SLE in the Asians
- no association between fcgammar2b and systemic lupus erythematosus in Swedish population
- results indicated that the presence of CD72-*2 allele decreases risk for human systemic lupus erythematosus conferred by FCGR2B-232Thr, possibly by increasing the AS isoform of CD72
- Signals underlying FcgammaRIIB-driven apoptosis are dependent on disruption of mitochondrial potential.
- results imply a novel functional role of CD13 and Fc gamma receptors (CD32 and CD64)as members of a multimeric receptor complex
- FcgammaRIIbl and b2 are both functional inhibitory receptors in the phagocytic process.
- Ile232Thr substitution affects the localization and function of FcgammaRIIB; this molecular mechanism may link the polymorphism and susceptibility to systemic lupus erythematosus.
- a recently described SLE-associated polymorphism of FcgammaRIIb (FcgammaRIIbT(232)), encoding a single transmembrane amino acid substitution, is functionally impaired
- Review of polymorphic screening studies of Japanese, Thai, and Chinese populations indicates that FCGR2B is a susceptibility gene associated with systemic lupus erythematosus.
- SHIP1 is necessary for FcgammaRIIB to negatively regulate B cell activation
- the FcgammaRIIB-50T-225C haplotype might be a susceptible factor of SLE in the Chinese population
- The response to either dimeric IgG or aggregated IgG (aIgG) was assessed and related to differences in the ratio of FcgammaRIIa to FcgammaRIIb2 mRNA in granulocytes
- FcgammaRIIb expression and synthesis occur throughout the placental vascular tree but do not extend into the umbilical cord.
- in human aortic endothelial cells, CRP upregulates monocyte-endothelial adhesion by activation of NF-kappaB through engaging the Fcgamma receptors CD32 and CD64.
- analysis of CD32B, which is expressed in B-cell lymphoma, and a panel of its monoclonal antibodies
- Unlike the Fc gamma RIIa receptor isoform, expression of Fc gamma RIIb is not detected in extracts of cultured human skin mast cell preparations.
- immature MPC-1- myeloma cells expressed CD32 more weakly than mature, MPC-1+ cells; Myeloma cells lacking CD19 expression are less sensitive to CD32-mediated growth suppression than are CD19+ normal plasma cells.
- Results suggest that FcgammaRIIB may be impaired at a critical checkpoint in systemic lupus erythematosus in the regulation of memory B cells.
- CD32-IgG interaction could directly drive capture and activation of flowing neutrophils, or potentiate activation of cells captured by other routes.
- Susceptibility to type 1 Autoimmune hepatitis in Japanese patients is not influenced by FcgammaRIIA, FcgammaRIIB, or FCRL3 polymorphisms.
- activating protein 1 has a role in the transcriptional regulation of the human FCGR2B promoter mediated by the -343 G -> C polymorphism associated with systemic lupus erythematosus
- FCGR2B 695T>C polymorphism is a critical determinant of severity in rheumatoid arthritis and is a potential therapeutic target.
- The FcgammaRIIb transmembrane polymorphism is a strong disease susceptibility candidate in epistasis with other genetic effects in Taiwanese and other Asian populations.
- FCGR2B expression is patently reduced in mature DCs, and is modulated by treatment with cytokines. The regulated expression of activating and inhibitory FcgRs in DCs emerges as a critical checkpoint in the process of Ag uptake and cross-presentation.
- Findings provide strong evidence suggesting that a FcgammaRIIB-Gln50Ter loci might be the susceptible factor of SLE in Chinese population.
- These results demonstrate that FcgammaRIIb is important in controlling the immune response to malarial parasites.
- Selective blockade of the inhibitory Fcgamma receptor (FcgammaRIIB) in human dendritic cells and monocytes induces a type I interferon response program.
- RA patients do not fail to up-regulate FcgammaRIIb upon synovial inflammation, but suggests that the balance between expression of the inhibitory FcgammaRIIb and activating FcgammaRs may be in favour of the latter throughout the disease course
- The researchers evidence of altered gene expression in the FCGR2B gene after both aerobic and exhaustive exercise.
- We demonstrate that the expression of the inhibitory receptors Fc gamma RII and CR1 is up-regulated on peripheral memory B cells of healthy controls, whereas this up-regulation is considerably impaired on the memory B cells of SLE patients.
- role of FcgammaRIIB and FcgammaRIIIA gene in susceptibility to systemic lupus erythematosus and to examine possible susceptible haplotypes between two genes
- Helicobacter pylori eradication shifts monocyte Fcgamma receptor balance toward inhibitory FCGR2B in immune thrombocytopenic purpura patients.
- association of the 232T allele with periodontitis might be related to lower levels of antibody response to Porphyromonas gingivalis
- Results identify FcgammaRIIB as a marker of human metastatic melanoma that impairs the tumor susceptibility to FcgammaR-dependent innate effector responses.
- Activation of human peripheral IgM+ B cells is transiently inhibited by BCR-independent aggregation of Fc gammaRIIB
- Neither activating receptors FcgammaRI and FcgammaRIII, nor FcgammaRIIB, all of which lack Phe(163), bound the peptide
- This is a report about a spurious (reitered) association between FCGR2B genetic polymorphisms and systemic lupus erythematosus.