|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for FABP4(NM_001442.2) Search again
Product ID:
HQP005072
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
A-FABP, AFABP, ALBP, HEL-S-104, aP2
Gene Description:
fatty acid binding protein 4
Target Gene Accession:
NM_001442.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq].
Gene References into function
- observations suggest that oxLDL-mediated increase in gene expression accelerate cholesterol ester accumulation, and that this is an important component of the genetic program regulating conversion of macrophages to foam cells
- fatty acid binding protein 4 and PPARgamma work together to influence a biologic pathway affecting insulin sensitivity and body composition
- intra-pair correlations revealed that FATP4 expression was signifcantly up-regulated in acquired obesity.
- a pre-lipolysis complex containing at least AFABP and HSL exists
- Loss of expression of A-FABP is associated with progression of bladder carcinoma.
- ALBP gene expression accelerates cholesterol and triglyceride accumulation in macrophage foam cells and affects some key gene expression for lipid metabolism.
- Data demonstrate a significant genetic variation in humans that results in decreased adipose tissue aP2 expression due to alteration of the CAAT box/enhancer-binding protein binding and reduced transcriptional activity of the aP2 promoter.
- FABP4 protein is expressed within the skeletal muscle fibers & FABP4 mRNA & protein are more abundant in the endurance trained subjects.[fatty acid binding protein 4]
- Expression occurs not only in mature adipocytes, but has a wider embryonic expression pattern than previously appreciated.
- the metallothionein genes, adipophilin (ADFP), CD36, adipocyte fatty acid binding protein (FABP4), ATP binding cassette protein A1 (ABCA1), and liver X receptor (LXR[alpha]) all emerged as strongly positively correlated with PPAR[gamma] expression.
- These data indicate for the first time that human macrophage aP2 promoter is a direct target for the regulation by LXR/RXR heterodimers.
- genetic variability at the FABP4 locus has been shown to be associated with plasma lipid levels, type 2 diabetes, and coronary heart disease risk
- Changes in DNA methylation at adipogenic promoters during cellular aging.
- thiazolidinedione increases FABP4 plasma concentrations in diabetic patients, reflecting PPARgamma activation
- Hypoxia enhances the expression of FABP4 in term human trophoblasts, suggesting that fatty acid binding proteins support fat accumulation in the hypoxic placenta.
- FABP4 concentrations should be taken into consideration as an early marker of kidney damage in patients with type 2 diabetes.
- human epicardial adipose and ascending aorta tissues express fatty-acid-binding protein 4 and that its level of expression in epicardial adipose tissues of metabolic syndrome patients is elevated
- High FABP4 & low adiponectin levels are independent predictors of atherogenic dyslipidemia. FABP4 plasma concentrations hold strong potential for development as a clinical biomarker for atherogenic dyslipidemia in type 2 diabetic subjects.
- Maternal AFABP serum concentrations are significantly increased in preeclampsia.
- A-FABP serum levels are positively associated with body weight and fat mass
- Mapping of the hormone-sensitive lipase binding site on the adipocyte fatty acid-binding protein (AFABP). Identification of the charge quartet on the AFABP/aP2 helix-turn-helix domain.
- Serum FABP4 levels increased as the numbers of stenotic coronary artery increased, although these differences were attenuated after adjustment for age and fasting glucose levels
- A-FABP is a candidate progression marker of human transitional cell carcinoma of the bladder that is differentially regulated by PPAR in urothelial cancer cells