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Validated All-in-One™ qPCR Primer for ERN1(NM_001433.4) Search again
Product ID:
HQP004992
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IRE1, IRE1P, IRE1a, hIRE1p
Gene Description:
endoplasmic reticulum to nucleus signaling 1
Target Gene Accession:
NM_001433.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq].
Gene References into function
- examination of luminal N-terminal ligand-independent dimerization domain
- HSP90 associates with the cytoplasmic domain of IRE1alpha
- the N-terminal luminal domain of IRE1 alpha has at least two distinct types of interactions mediating dimerization and function in signaling
- ATF6 and Ire1p signaling do not define the magnitude of UPR-dependent mRNA increases, even though they may be necessary for gene activation.
- JAB1 may act as a key molecule in selecting the unfolded protein response or cell death by association and dissociation with IRE1alpha
- hepatitis B virus S promoter responds to a novel, cell type-restricted transcriptional pathway downstream of IRE1-alpha and XBP1
- These results suggest that IRE1alpha-mediated mRNA cleavage functions even in mammals as a common system to regulate gene expression.
- IRE1 signaling is essential for ischemia-induced vascular endothelial growth factor-A expression and contributes to angiogenesis and tumor growth in vivo
- study found IRE1 attenuated by ER stress; when IRE1 activity was sustained artificially, cell survival was enhanced, suggesting a causal link between duration of unfolded protein response branch signaling & life or death cell fate after ER stress
- ER stress increases MTHFR expression and IRE1 and c-Jun mediate this activation
- RNase domain of IRE1 determines the functional specificities of each of these isoforms.
- Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the IRE1
- USP14 interacted with the cytoplasmic region of IRE1alpha, and the endogenous interaction between USP14 and IRE1alpha was inhibited by ER stress.