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Validated All-in-One™ qPCR Primer for ENO2(NM_001975.2) Search again
Product ID:
HQP004864
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HEL-S-279, NSE
Gene Description:
enolase 2
Target Gene Accession:
NM_001975.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq].
Gene References into function
- Serum level of enolase 2 heavily influences survival in neuroblastoma in children.
- expressed in the placenta and umbilical cord
- Serum time course of two brain-specific proteins, alpha(1) brain globulin and neuron-specific enolase, in tick-born encephalitis and Lyme disease.
- neuron-specific enolase (NSE) may have a role in development of pyothorax-associated lymphoma (PAL)
- the frequency of a high NSE serum value in acute and lymphoma type adult T-cell leukemia (ATL) suggests that ATL cells preferentially produce NSE compared with other NHL cells
- Patients with viral meningitis showed high concentrations of inosine, guanosine, xanthine, urate and neuron-specific enolase.
- Increased level of neuron-specific enolase is associated with small-cell lung cancer and non-small cell lung cancer patients
- Decreasing levels of serum NSE but not S-100B over time may indicate selective attenuation of delayed neuronal death by therapeutic hypothermia in victims of cardiac arrest
- enzyme has been purified, crystallized and its crystal structure determined.
- NSE increases to similar degrees with and without traumatic brain injury
- The crystal structure of the human neuronal enolase-Mg2F2P(i) complex determined at 1.36 A resolution shows that the combination of anions effectively mimics an intermediate state in catalysis
- Serum neuron-specific enolase (NSE) measurement has prognostic value in predicting outcomes in patients recovering from cardiopulmonary resuscitation following in-hospital cardiac arrest.
- MS patients had a greater prevalence of positive T-cell proliferative responses to neuron-specific enolase [NSE], retinal arrestin, and beta-arrestin than healthy controls (p<0.0001).
- Neuron-specific enolase is a relevant marker in investigation of the patients with neurofibromatosis type 1 and associated tumors
- Serum samples were investigated for carcinoembryonic antigen (CEA), CA125 and MUC1, alpha-foetoprotein, neuron-specific enolase and CA19.9.
- Early upregulation of neuron-specific enolase in children with head injury, correlates significantly with the severity of the head injury.