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Validated All-in-One™ qPCR Primer for ENO1(NM_001428.2) Search again
Product ID:
HQP004860
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH
Gene Description:
enolase 1
Target Gene Accession:
NM_001428.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message.
Gene References into function
- Proteomic analysis of human brain identifies alpha-enolase as a novel autoantigen in Hashimoto's encephalopathy.
- A target of excess protein nitration in Alzheimer disease (AD) brain, providing evidence of the importance of oxidative stress in AD progression
- The alpha-enolase protein is the target protein of serum anti-endothelial antibody in Behcet's disease patients.
- antibodies against human alpha-enolase target antigens in retinal ganglion cells and inner nuclear layer cells and induce the apoptotic death of sensitive cells in rat retinal explants
- Results suggest a novel role of c-myc promoter-binding protein 1 for suppression of prostate cancer cell growth by regulating the MEK5-mediated signaling pathway.
- ENO1 has tumour suppressor activity and high level of ENO1 expression has growth inhibitory effects
- Depletion of MBP-1 in prostate cancer cells perturbs cell proliferation by inhibiting cyclin A and cyclin B1 expression.
- Our results indicate that differential subcellular localization of ENO1 products may be closely related to carcinogenesis of the oral epithelium
- alpha-crystallin B, glyceraldehyde phosphate dehydrogenase (GAPDH), and alpha-enolase identified as significantly S-glutathionylated in Alzheimer's disease infeior parietal lobule
- These findings further support the distinct roles of alpha-enolase and its MBP-1 variant in maintaining cell homeostasis. Moreover, these data suggest a novel function for NS1-BP in the control of cell proliferation.
- alpha-enolase and MBP-1 associate with the kelch protein NS1-BP. c-Myc gene transcriptional repression exerted by MBP-1 is enhanced by NS1-BP.
- Enolase on the surface of cells may be a receptor for plasminogen. The enolase/plasminogen (plasmin) system is one of the mechanisms facilitating the invasiveness of pathogens and it plays a role in myogenesis and in the development of tumor tissues.
- TGF-beta1-induced growth arrest was associated with notable downregulation of the myc-binding protein-1 (MBP-1).
- These results indicate that the activated Notch1 receptor and alpha-enolase or MBP-1 cooperate in controlling c-myc expression through binding the YY1 response element of the c-myc promoter to regulate tumorigenesis.
- Crystal structure of ENO1 is presented at 2.2 A resolution. Despite its high sequence similarity to other enolases, the ENO1 structure exhibits distinct surface properties, explaining its various activities, including plasmin(ogen) and DNA binding.
- Alpha-enolase was citrullinated in rheumatoid arthritis synovial fluid.
- demonstration that IL8, DES and ENO1 act as the central elements in colon cancer susceptibility, and protein biosynthesis and the ribosome-associated function categories largely account for the colon cancer tumuorigenesis
- HIF-1alpha-regulated glycolysis module is closely related to the aggressive phenotype of hepatocellular carcinoma, and ENO1, a glycolysis module gene, might have a role in preventing metastasis
- Crystals of alpha-Enolase from human liver were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution.