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Validated All-in-One™ qPCR Primer for CTTN(NM_005231.3) Search again
Product ID:
HQP004839
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EMS1
Gene Description:
cortactin
Target Gene Accession:
NM_005231.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq].
Gene References into function
- substrate for caspase cleavage during apoptosis
- Primary arrest of circulating platelets on collagen involves phosphorylation of Syk, cortactin and focal adhesion kinase
- The Src-cortactin pathway is required for clustering of E-selectin and ICAM-1 in endothelial cells
- Cdc42/Rac1-dependent activation of PAK may trigger early platelet shape change, at least in part through the regulation of cortactin binding to PAK.
- cortactin links receptor endocytosis to actin polymerization by binding both CD2AP and the Arp2/3 complex, which may facilitate the trafficking of internalized growth factor receptors
- alternative splicing of the F-actin binding domain of cortactin is a new mechanism by which cortactin influences cell migration
- cortactin and EC myosin light chain kinase have roles in mediating lung vascular barrier augmentation evoked by S1P
- RhoGAP functionally interacts with cortactin and represents a novel determinant in the regulation of cell dynamics.
- Data suggest that hyaluronan-CD44 interaction with Rac1-protein kinase N gamma plays a pivotal role in phospholipase C gamma1-regulated calcium signaling and cortactin-cytoskeleton function required for keratinocyte cell-cell adhesion and differentiation
- HAX-1 binds bile salt export protein (BSEP), cortactin, MDR1, and MDR2. HAX-1 and cortactin regulate BSEP abundance in the apical membrane of cells.
- Erk phosphorylation liberates the SH3 domain of cortactin from intramolecular interactions with proline-rich regions, causing it to synergize with WASP and N-WASP in activating the Arp2/3 complex
- Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery.
- a major role for a Crk-cortactin complex in actin polymerization downstream of tyrosine kinase signaling
- Overexpression of cortactin may play a role in the metastasis of HCC by influencing cell motility, and cortactin could be a sensitive marker for HCC with intrahepatic metastasis.
- cortactin can restrict N-WASP localization around EGF-bead-induced protrusions
- The p47phox:actin interaction, through cortactin, plays an important role in Ang II-mediated site-directed assembly of functionally active NAD(P)H oxidase, ROS generation, and activation of redox-sensitive p38MAP kinase and Akt, but not ERK1/2.
- Cortactin promotes cell motility by enhancing lamellipodial persistence, at least in part through regulation of Arp2/3 complex.
- Cortactin recruitment is dependent on the activation of a phosphoinositide-3-kinase/Rac1-GTPase signalling pathway, which is required for actin polymerization and internalization of N. meningitidis.
- Our results demonstrated the dominant expression of p85 form of cortactin in colorectal cancer for the first time.
- cortactin is the crucial gene within the 11q13 amplicon that mediates the invasive potential of human carcinomas
- Tyrosine phosphorylation of cortactin by Src family kinases regulates neutrophil transmigration.
- We describe that S1P-stimulated cortactin translocation to the cell periphery to form lamellipodia is specifically mediated by the endothelial S1P1 G-protein coupled receptor, and is regulated by G(i)-mediated Akt-dependent S1P1 receptor phosphorylation.
- Co-localization of cortactin and phosphotyrosine identifies invadopodial complexes closely associated with the plasma membrane at active sites of focal degradation of the extracellular matrix in breast cancer cells.
- Our findings suggest that cortactin plays a role in Listeria internalization, but not in the formation of actin clouds and tails, or in bacteria intracellular motility.
- Increased levels of cortactin, as found in human carcinomas, promote cell migration and invasion by reducing cell spreading and intercellular adhesive strength.
- Cortactin is a requirement for pedestal formation and suggest a novel function for the predicted alpha-helical region of cortactin in actin assembly induced by EPEC and EHEC.
- Results suggest that the AMAP1/cortactin complex, which is not detected in normal mammary epithelial cells, is an excellent drug target for cancer therapeutics.
- functional importance of cortactin to CD44s-promoted metastasis was demonstrated by selective suppression of cortactin in CD44-expressing MCF7F-B5 and MDA-MB-231 breast cancer cells using RNAi
- EMS1 amplification is uncommon and appears to be late event in the development of ethmoid sinus adenocarcinoma.
- The identification of 17 new sites of phosphorylation in cortactin is reported.
- Results suggest that ATP-mediated barrier protection is associated with cytoskeletal activation and is dependent on both Rac activation and cortactin.
- Plays important role in CXCR4 signaling and trafficking as well as in receptor-mediated cell migration.
- Controls osteoclastic bone resorption by regulating actin organization.
- data suggested potential roles in oral carcinogenesis and that TAOS1 might be involved earlier than EMS1. Both genes might be candidate biomarkers for diagnosis and prognosis in OSCC
- Tyrosine phosphorylation of cortactin dynamically links ICAM-1 to the actin cytoskeleton, enabling ICAM-1 to form clusters and facilitate leukocyte transmigration.
- CTTN is an oncogene in the 11q13 amplicon and exerts functions on tumor metastasis in esophageal squamous cell carcinoma.
- cortactin-mediated signaling pathways, with emphasis on its contribution to tumor progression and metastasis formation.[REVIEW]
- These results indicate that cortactin is involved in keratinocyte migration promoted by both KGF and FGF10.
- Cortactin binding to EHEC virulence factors, Tir and EspF(u), is dependent upon tyrosine phosphorylation during infection.
- A major role of cortactin in invadopodia is to regulate the secretion of MMPs and point to a novel mechanism coupling dynamic actin assembly to the secretory machinery, producing enhanced ECM degradation and invasiveness.
- Higher intensity of cortactin and fascin-1 staining correlated directly with more-advanced cancer stages (TNM) and inversely with survival rates.
- cortactin and actin have roles in hyperoxia-induced activation of NADPH oxidase and ROS generation in human lung endothelial cells
- Cortactin and p120 are shown to directly interact with each other via the cortactin N-terminal region
- Using a variety of approaches including siRNA, expression of dominant negative derivatives and pharmacological inhibitors, Results demonstrate the crucial role of cortactin in the activation of the Arp2/3 complex during InlA-mediated entry.
- in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin
- CD44 is an important regulator of HGF/c-Met-mediated in vitro and in vivo barrier enhancement, a process with essential involvement of Tiam1, Rac1, dynamin 2, and cortactin.
- cortactin-mediated actin remodeling in excitable cells is not only important for cell structure, but may directly impact membrane excitability
- tyrosine protein phosphorylation regulates large conductance voltage- and calcium-activated potassium channels via cortactin
- potential mechanisms by which cortactin may link vesicular trafficking and dynamic branched actin assembly to regulate protease secretion for invadopodia-associatedextracellular matrix degradation
- PTP1B regulates cortactin tyrosine phosphorylation by targeting Tyr446
- These results suggest that cortactin hyperphosphorylation suppresses cell migration possibly through the inhibition of membrane localization and tyrosine phosphorylation of p130Cas.
- many studies have documented a role for cortactin in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the extracellular matrix by cancer cells.
- CTTN gene variation may contribute to severe asthma
- Overexpression of cortactin is associated with head and neck squamous cell carcinomas
- These results imply that cortactin is recruited to the site of EHEC adhesion in vitro and ex vivo by different mechanisms and suggest that cortactin might have a role during EHEC infection of mucosal surfaces.
- Cortactin, fascin-1 and EGFR have roles in progression of ovarian carcinomas
- Overexpression of cortactin is associated with head and neck squamous cell carcinoma.
- The strong relationship of CTTN amplification/overexpression with prognosis and disease outcome reinforces its role as a driver of 11q13 amplification in HNSCC.