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Validated All-in-One™ qPCR Primer for ELAVL1(NM_001419.2) Search again
Product ID:
HQP004694
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ELAV1, HUR, Hua, MelG
Gene Description:
ELAV like RNA binding protein 1
Target Gene Accession:
NM_001419.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression. [provided by RefSeq].
Gene References into function
- role in c-fos mRNA export
- A 40-bp RNA element that mediates stabilization of vascular endothelial growth factor mRNA by HuR. (HuR RNA binding protein)
- Results show that AMP-activated kinase (AMPK), an enzyme involved in responding to metabolic stresses, potently regulates the levels of cytoplasmic HuR.
- The binding of HuR, CP1, and CP2 to AR mRNA suggests a role for each of these proteins in the post-transcriptional regulation of AR expression in cancer cells.
- Data show that the RNA-binding protein HuR is specifically methylated on Arg(217) by coactivator-associated arginine methyltransferase 1 (CARM1).
- Highly selective actions of HuR in antagonizing AU-rich element-mediated mRNA destabilization.
- p21(WAF1) protein expression is mediated by stabilization of p21(WAF1) mRNA elicited via multiple 3'-UTR cis-elements. HuR binds at least one of these elements, does not appear to be a modulator of p21(WAF1) expression or growth inhibition in this system
- AMP-activated protein kinase activation can cause premature fibroblast senescence through mechanisms that likely involve reduced HuR function.
- The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR plays a role in binding to the p53 mRNA and enhancing its translation
- HADHB, HuR, and CP1 are novel REN mRNA-binding proteins that target a cis-element in the 3'-UTR of REN mRNA and regulate renin production
- HuR has a role in enhancing VHL-mediated p53 translation
- HuR and MK2 have roles in regulating the expression of uPA and uPAR genes at the posttranscriptional level
- Overexpression of HuR in bronchial epithelial cells significantly increases the expression and stability of eotaxin mRNA and protein.
- HuR-regulated target mRNA expression contributes to colon cancer growth.
- AUF1 p40, HuR, and the 3'UTR of the CTR mRNA transcript could be involved in posttranscriptional regulation of CTR mRNA expression.
- Transportin-2 mediates nuclear import of HuR protein in vitro
- an HuR target RNA motif and a general strategy for identifying target motifs for RNA-binding proteins
- composition and fate (stability, translation) of HuR- and/or AUF1-containing ribonucleoprotein complexes depend on the target mRNA of interest, RNA-binding protein abundance, stress condition, and subcellular compartment
- Overexpression of HuR in tumor tissue may be part of a regulatory pathway that controls the mRNA in breast cancer
- Results point to importin alpha1 as a critical downstream target of AMP-activated protein kinase (AMPK) and key mediator of AMPK-triggered HuR nuclear import.
- PGA2 stabilizes the p21 mRNA through an ERK-independent increase in cytoplasmic HuR levels and an ERK-dependent association of HuR with the p21 mRNA
- IL-8 RNA from IL-1beta-stimulated cytoplasmic extract revealed a 20-fold greater association of transcript with the stabilizing factor HuR in breast csancer cells.
- HuR contributes to regulating human vascular smooth muscle cell growth and homeostasis in pathologies associated with vascular smooth muscle proliferation
- The function of HuR as a 5'-UTR-binding protein and dual-purpose translation repressor may be critical for the precise regulation of type I insulin-like growth factor receptor expression.
- coordinated regulation of mRNA stability by HuR and AUF1 proteins contributes to the observed increase in ATF3 expression following amino acid limitation
- HuR is a key mediator of posttranscriptional regulation and expression of the SLC11A1 gene.
- c-Yes 3'-UTR contains at least three newly identified adenine/uridine-rich elements (AREs) which are bound specifically by ARE-binding proteins HuR and AUF1.
- HuR-mediated mRNA stabilization, stimulated by integrin engagement and controlled at the level of HuR nuclear export, is critically involved in T cell activation
- the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions
- The mRNAs for GM-CSF and TNF-alpha - established ARE-containing targets for HuR-mediated regulation - were upregulated by LPL-mediated lipolysis in ECAP.
- HUR maintains cytochrome c biosynthesis.
- role for HuR in protecting kidney epithelia from injury during ischemic stress
- Results describe the gene expression of tristetraprolin, T-cell intracellular antigen and Hu antigen R in synovial tissues from rheumatoid arthritis and osteoarthritis patients.
- adenosine/uridine (AU)-rich element-binding protein HuR (Hu antigen R) interacts with the beta-F1-ATPase mRNA through an AU-rich sequence located to the 3'-UTR.
- HuR critically contributes to cyclin E1 overexpression and its growth-promoting function in breast cancer cells.
- IL-10 sensitivity to TNF depends on the ability of IL-10 to inhibit the expression and mRNA-stabilizing protein HuR and via IL-10 mediated repression of p38 mitogen-activated protein (MAP) kinase activation
- Activation of NADPH oxidase and MAPK-signaling pathway contribute to HuR-mediated stabilization of TLR4 mRNA induced by LPS in human aortic smooth muscle cells (HASMCs). Stimulation of HASMCs with LPS increased the cytosolic HuR level in vitro.
- The RNA-binding protein HuR regulates the stability and translation of target mRNAs, while no HuR mutations have been found in cancer, a link between HuR and malignant transformation has been suggested in cancers of the breast, colon, lung and ovary.
- the signal sequences in APRIL that mediate its intracellular trafficking and provide evidence that this protein ligand of HuR is an important player in the post-transcriptional regulation of CD83 expression
- HuR-dependent regulation of RNase-L enhances its antiviral activity, demonstrating the functional significance of this regulation
- HuR regulates SIRT1 expression, underscore functional links between the two stress-response proteins, and implicate Chk2 in these processes.
- Data show that regulation of HuR via protein kinase C alpha-dependent phosphorylation emphasizes the importance of posttranslational modification for stimulus-dependent HuR shuttling.
- analysis of distinct roles for the HuR hinge and RRM3 domains in formation of cooperative HuR.ARE complexes in solution
- Down regulation of HuR expression by siRNA prevents atazanavir-induced increase of TNF-alpha and IL-6, suggesting that HuR might have an impact on pathophysiological processes of HIV PI-induced atherosclerosis
- Review. HuR binds to & promotes the expression of mRNAs for Bcl-2 & Mcl-1. HuR is a key upstream coordinator of a constitutive pro-survival program.
- These data establish that the posttranscriptional event involving HuR-mediated beta-actin mRNA stabilization could be a part of the regulatory mechanisms responsible for maintaining cell integrity.
- Cytoplasmic HuR expression is significantly associated with tumor size, stage, and lymphatic/vascular invasion in uterine cervical carcinoma and is correlated with cyclooxygenase 2 expression.
- Positive correlations between Hu antigen R gene expression in patients with rheumatoid and healthy persons.
- Two mRNA binding proteins, HuR and AUF1, are colocalized and are capable of functional interaction in both the nucleus and cytoplasm.
- both HuR and AUF1 bind to discrete regions of DENR/DRP mRNA and that AUF1 silencing increases DENR/DRP protein levels.
- HuR and PTB jointly upregulate HIF-1alpha translation in response to CoCl(2)
- There was a statistically significant correlation between COX-2 immunoreactivity and the cytoplasmic HuR expression level in laryngeal squamous cell carcinoma.
- The role of cytoplasmic expression of HuR is as a biomarker for progression of adenomas in familial polposis coli.
- Primary Merkel cell carcinomas and their lymph node metastases as well as non-neoplastic skin show nuclear expression of HuR protein.
- HuR association with pp32/PHAP-I and its caspase-mediated cleavage constitutes a regulatory step that contributes to an amplified apoptotic response.
- the subcellular localization of HuR is deregulated in a subset of prostate carcinomas, and that this deregulation is linked to an altered expression of the tumorigenic COX-2 protein as well as to an adverse patient prognosis
- Mitogen-induced interleukin (IL)-13 expression involves changes in mRNA transcript turnover and a change in phosphorylation of Hu antigen R (HuR) and its association with the mRNA 3'-untranslated regions.
- Posttranslational modification of HuR by protein kinase Cdelta represents an important novel mode of HuR activation implied in renal COX-2 regulation
- HuR is involved in the upregulation of VEGF-A expression in human meningiomas.
- Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4
- UVB irradiatiion regulates COX2 mRNA stability through AMPK and HuR in human keratinocytes.
- a functional link between HuR as repressor of alternative Fas splicing and the molecular mechanisms modulating programmed cell death.
- MKP-1 upregulation by oxidative stress is potently influenced by increased mRNA stability and translation, mediated at least in part by the RNA-binding proteins HuR and NF90.
- These findings suggest that the expression of VEGF-A and cytoplasmic translocation of HuR relates to the histological grade, and that HuR is involved in the upregulation of VEGF-A expression, in human astrocytic tumors.
- absence of two critical adenosine/uridine-rich elements in the alternatively spliced CD3zeta 3'-UTR found in systemic lupus erythematosus T cells may result in decreased HuR binding
- These results suggest that HuR may be involved in and may modulate the reverse transcription reaction of HIV-1, by an as yet unknown mechanism involving a protein-protein interaction with HIV-1 reverse transcriptase.
- HuR plays an important role in suppression of TM protein synthesis in IL-1beta treatment and sepsis
- Cdk1 phosphorylates HuR during G2, thereby helping to retain it in the nucleus in association with 14-3-3 and hindering its post-transcriptional function and anti-apoptotic influence.
- global changes in HuR-bound mRNAs are implicated in the evolution to a more tumorigenic phenotype in an in vivo tumor model.
- The mRNA stability factor HuR was shown to support ERBB2 transcript integrity, bind and endogenously associate with a conserved U-rich element within the ERBB2 transcript 3' UTR, and colocalize with HDAC6.
- overexpression of HuR as found in many progressive tumors could be causative for disarranged Eph receptor to ephrin ligand ratios leading to a higher degree of tissue invasiveness
- UV cross-linking and immunoprecipitation experiments revealed 2 ARE-binding proteins, AUF1 and HuR, associated with IL-8 mRNA in saliva.
- COX-2 expression in Ewing sarcoma may not be directly related to mRNA stabilization by HuR. However, a correlation between COX-2 expression and nuclear HuR expression through indirect mRNA stabilization can be suggested.
- Cytoplasmic accumulation of HUR is central to tamoxifen resistance in estrogen receptor positive breast cancer cells.
- HuR is a direct transcription target of NF-kappaB; its activation in gastric cancer cell lines depends on phosphatidylinositol 3-kinase/AKT signaling. HuR activation by this pathway has proliferative and antiapoptotic effects on gastric cancer cells.
- HuR plays a role in tumor progression in mesothelioma and that COX-2 may be a target of its activity
- In differentiated cervical epithelial cells HuR may bind and stabilise L1 mRNAs aiding translation of L1 protein.
- In the present report we show that the RNA-binding protein ELAV/HuR can affect, post-transcriptionally, the fate of HSP70 mRNA following H(2)O(2)-mediated oxidative stress in SH-SY5Y human neuroblastoma cells.
- High expression of HuR is associated with colorectal adenocarcinoma.
- Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR.
- the growth-promoting effects of antisense miR-519 required the presence of HuR
- These findings suggest that HuR actively contributes to RNA modification and maturation and thereby shed an entirely new light on the role of HuR in RNA metabolism.
- identified a distinct AU-rich element in the 3'UTR of eIF4E which is responsible for HuR-mediated binding and stabilization
- An RNA-binding region of human HuR containing two N-terminal RNA-recognition motif domains bound to an 11-base RNA fragment has been crystallized.