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Validated All-in-One™ qPCR Primer for EGR1(NM_001964.2) Search again
Product ID:
HQP004612
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Gene Description:
early growth response 1
Target Gene Accession:
NM_001964.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
Gene References into function
- Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma.
- Early Growth Response-1 gene mediates up-regulation of epidermal growth factor receptor expression during hypoxia
- HIV Tat binds Egr proteins and enhances Egr-dependent transactivation of the Fas ligand promoter
- Egr-1 gene of transfected HHCC and ECa109 cells showed obvious suppression of the cell growth and malignant phenotypes, but no suppression in SMMC7721 (HCC cell line) cells.
- Activation of human monoamine oxidase B gene expression by a protein kinase C MAPK signal transduction pathway involves c-Jun and Egr-1.
- role in mediating tissue factor expression in endothelial cells induced by CD40 ligand
- Egr-1 is necessary and sufficient to activate human PPARgamma1 gene expression in VSMC
- Egr1 has multiple roles in prostate tumor cell growth and survival, cell differentiation, tumor progression, angiogenesis, and apoptosis.
- Egr1 translates developmental signals into appropriate changes in gene expression at multiple stages of thymocyte development.
- Egr1 is the oncostatin M-induced transcription factor that binds to the SIRE sequence of the LDL receptor promoter
- transactivation of the EGF receptor as well as stimulation of the mitogen activated/extracellular signal-regulated protein kinase (ERK) are essential for substance P/NK-1 receptor-induced activation of Egr-1 biosynthesis
- The proximal serum response element in the Egr-1 promoter mediates response to thrombin in primary human endothelial cells. Thrombin does this by a MAPK-dependent mechanism that involves an interaction between SRF & SRE-1.
- Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.
- studies for the first time identify Egr-1 as a nuclear target of gastrin and show that functional interplay of Egr-1, Sp1, and CREB is indispensable for gastrin-dependent CgA transactivation in gastric epithelial cells
- Data demonstrate that Egr-1, AP1 and Smad are part components of the transforming growth factor beta signal transduction pathway that regulates PPAR gamma expression.
- Keratinocytes of chronic psoriatic plaques were characterized by enhanced p16, p14(ARF), and p12 expression accompanied by elevated Egr-1 levels
- Overexpression of EGR1 via generation of superoxide anions inhibits the basolateral Na+/K+-ATPase activity of kidney proximal tubule cells.
- role of Egr-1 in lipopolysaccharide (LPS) induction of TF and inflammatory mediators in vivo was evaluated using Egr-1(+/+) and Egr-1(-/-) mice
- Egr-1 has a significant role in carcinogenesis and in cancer progression, especially metastasis.
- upregulation of Egr-1 might contribute to fibrosis observed in rheumatoid arthritis synovium by activation of genes encoding the alpha1 and alpha2 chains of type I collagen
- PI3K/ERK signaling by the EP(4) receptors induces the functional expression of early growth response factor-1 (EGR-1)
- The enhanced expression of Egr-1 may regulate the activity of matrix metalloproteinases in synovial fibroblasts by enhancing the expression of the TIMP-1, -2, and -3 genes.
- data demonstrates that eukaryotic translation initiation factor 4E binding protein 1 is a new target for early growth response-1
- Egr-1 represses COL2A1 by preventing interactions between Sp1 and the general transcriptional machinery
- Egr1 controls p21Cip1 expression by directly interacting with a specific sequence on its gene promoter.
- These results suggest that induction of egr-1 may be needed to regulate genes involved in DNA repair, cell survival, and apoptosis.
- stimulation by ATP via a protein kinase C-dependent pathway in the human osteoblastic HOBIT cell line
- study provides the first evidence of the crucial role of Egr-1 in microvascular endothelial cell growth, neovascularization, tumor angiogenesis and tumor growth
- EGR1 binds to the androgen receptor (AR) in prostate carcinoma cells, and an EGR1-AR complex can be detected
- study provides strong evidence that early growth response 1 regulates low density lipoprotein receptor transcription via a novel mechanism of protein-protein interaction with CCAAT enhancer-binding protein beta
- activation of JNK and expression of Egr-1 gene in B lymphoblasts mediate a cellular response to genotoxic agents that may be induced by Fanconi anemia proteins
- early growth response 1 regulates inducible heparanase gene transcription in activated T cells
- EGR-1 is required for expression of NAG-1 by troglitazone
- Egr-1-mediated activation of transcription is regulated by mPGES-1 in inflammatory bowel disease
- expression of Egr-1, c-fos and cyclin D1 varies in esophageal precancerous lesions and cancer tissues, suggesting an involvement of these genes in the development of esophageal carcinoma.
- microtubules regulate EGR-1 localization in benign prostate cells but not in malignant prostate cells
- Although EGR-1 is induced by PDGF in human airway smooth muscle cells in cell culture, the role of EGR-1 in airway remodeling and asthma remains to be established.
- mPGES-1 and Egr-1 are novel targets of PPARgamma
- Helicobacter pylori induced expression of Egr-1 in gastric epithelial cell lines in a dose-dependent manner, suggesting a role, in part, in Helicobacter pathology
- Review examines emerging evidence that ERG-1 orchestrates transcriptional response to vascular injury.
- Cells induced with serum to increase endogenous Egr1 increase the transcription of p300/CBP only when Egr1 binding sites in the promoter are not mutated, causing the expression of downstream targets of Egr1.
- Egr-1 may display tumor-suppressing activity and offers a potential target for uterine leiomyoma management.
- Deletion analyses of the Egr-1 promoter identify a minimal estradiol-responsive region of the promoter containing a serum response element which binds Elk-1 and serum response factor.
- Findings suggest that EGR-1 expression and activity is differentially regulated in prostate cancer cell line PC-3 and BPH-1 cell line.
- EGR-1 regulates PTEN expression in the initial steps of the apoptotic pathway
- results show that there are genuine Sp1/Sp3 or Egr-1 controlled genes showing no cross-regulation of Sp1/Sp3 and Egr-1 through the same DNA-binding site
- Egr-1 has roles in regulating both the basal and LPS-induced activity of the SOCS-1 promoter
- EGR1 is a significant contributor to mutant p53 gain of function
- the MEK1/ERK1/2 pathway couples the CCK2 receptor to nuclearization and DNA binding of Egr-1
- IL-17-stimulated TNF-alpha mRNA expression and promoter activity was dependent on Egr-1 expression.
- Data show that ionizing radiation causes stress-induced activation of insulin-like growth factor-1 receptor-Src-Mek-Erk-Egr-1 signaling that regulates the clusterin pro-survival cascade.
- Up-regulation of EGR1 contributed significantly more to heparanase expression than did promoter CpG hypomethylation in prostate cancer samples.
- finding of FGF2 and EGR1 upregulation in endothelial cell microvascular channels within organising thrombus
- results show that distal serum response elements within the Egr-1 promoter are of major importance for the inducibility of Egr-1 gene transcription by tetradecanoylphorbol acetate
- TNF-alpha-induced CD44 expression was regulated by AP-1 through the activation of the CaMK-II pathway, whereas LPS-induced CD44 transcription was regulated specifically by Egr-1 through JNK activation.
- The EGR1 gene appeared to be deleted in ER-negative human breast carcinomas. Egr-1 may contribute to the pathogenesis of ER-negative breast carcinomas versus ER-positive breast carcinomas.
- EGR1 expression in combination with promotor CpG methylation regulates heparanase expression
- this is a novel first report demonstrating that the expression of ATF3 occurs via early growth response gene-1 downstream of extracellular signal-regulated kinase1/2
- c-Abl promotes the induction of EGR1 through the MEK/ERK pathway in regulating apoptotic response to oxidative stress.
- EGR1 regulates heparanase transcription in tumor cells and importantly, can have a repressive or activating role depending on the tumor type
- Egr-1 induces the expression of its corepressor nab2 by activation of the nab2 promoter in a negative feedback loop
- Nkx3.1 and Egr1 regulate gene programs involved in distinct aspects of prostate tumorigenesis [review]
- Overexpression of Egr-1, but not Egr-3, is capable of augmenting transcription of an intact CD154 promoter.
- Transcriptional stimulation of the human NHE3 promoter activity by PMA: PKC independence and involvement of the transcription factor EGR-1.
- The IGFBP3, hRas, JunB, Egr-1, Id1 and MIDA1 genes were up-regulated in psoriatic involved skin compared with uninvolved skin.
- EGR-1 is induced by transforming growth factor-beta and mediates stimulation of collagen gene expression
- Expression of Tat decreased NGF-induced Egr-1 levels in neuroblastoma cells.
- EGR1 regulates TOPBP1 gene transcription.
- These findings indicate that Snail upregulation by HGF is mediated via the MAPK/Egr-1 signaling pathway and that both Snail and Egr-1 play a critical role in HGF-induced cell scattering, migration, and invasion.
- GTP-Binding Protein alpha Subunits, Gq-G11, gene is regulated during megakaryocytic differentiation by EGR-1.
- The identification that induction of MMP-2 and MT1-MMP by CSE from lung fibroblasts is EGR-1-dependent reveals a molecular mechanism for matrix remodeling in cigarette smoke-related emphysema.
- cDNA microarray analysis revealed that expression of early growth response gene-1 (Egr-1) was increased by LMP1 expression in MCF7 and Jurkat cells.
- Haploinsufficiency for Egr1plays a role in the development of acute myeloid leukemia and myelodysplastic syndromes characterized by abnormalities of chromosome 5.
- EGR1 is upregulated after radical prostatectomy for prostatic neoplasms.
- The MAPK-EGR-1-HSP70 pathway regulates the cigarette smoke-induced inflammatory process.
- Can repress herpes simplex virus (HSV) type 1 immediate-early gene expression, and thus may play a critical role in HSV-1 gene silencing during latency and reactivation.
- reveal a novel role for protein phosphatase 1 inhibitor in the regulation of the tumor suppressor gene EGR1 via activation of the MEK-ERK MAPK pathway
- Radiofrequency radiation (900 MHz) induces EGR1 gene expression and affects cell-cycle control in human neuroblastoma.
- STI571 influence through inducing urokinase-type plasminogen activator and interleukin-8, in which the induction of early growth response-1 expression and extracellular signal-regulated kinase 1/2 phosphorylation might be involved.
- Constitutive expression of Egr-1 transgene abrogates E2F-1 oncogene-mediated block in differentiation of murine myeloblastic leukemia cells, allowing the cells to differentiate into functionally mature macrophages that subsequently undergo apoptosis.
- Our data demonstrate that IL-1beta-induced Id2 expression in VSMC is mediated by the transcription factor Egr-1 in VSMC.
- Results reveal that egr-1 is a target gene of PGE(2) in HCA-7 cells and is regulated via the newly identified EP4/ERK/CREB pathway.
- Calcipotriol affects proliferation of keratinocytes by decreasing the expression of EGR1 and PLK2.
- Activated protein C may provide cytoprotective activity by activating the ERK pathway, which upregulates EGR-1 thereby suppressing the expression of TRAIL by PAR-1/S1p1 dependent but EPCR independent mechanism.
- Hemin increased Egr-1 expression (mRNA, protein) within 30 minutes and ERK-1/2 phosphorylation and nuclear translocation within 5 minutes
- These results indicate that EGR1 is a key player in coordinated down-regulation of genes in leiomyoma.
- defect in upregulation of p57Kip2 transcription is due to reduced levels of EGR1 caused by direct, destabilizing interaction with PAX3-FOXO1
- Egr-1 negatively regulates survivin expression and sensitizes cell lines to TRAIL-induced apoptosis
- These results suggest that elevated expression and activity of PLD attenuate phorbol myristate acetate-induced Egr-1 expression via PI3K pathway.
- Intact SP1-binding sites are essential for promoter activity, whereas EGR-1 suppresses the transcription of the human SREBP-1a promoter
- Antithrombin might inhibit LPS-induced production of TNF-alpha by inhibiting the increase in Egr-1 expression in monocytes via interaction with heparin-like substances expressed on the cell surface
- The greatest reduction in the number of C. pneumoniae inclusions was due to the silencing of the gene coding for the transcription factor early growth response 1, which decreased the number of inclusions by 38.6%
- Prolonged exposure to heme, as may be observed after plaque rupture in atherosclerotic disease, leads to dysregulation and prolonged elevated Egr-1 expression that contributes to atherosclerotic progression
- These results suggest that Egr-1 may be an important breast cancer marker and that an as yet uncharacterized pathway involved in Egr-1 and gelsolin expression exists which leads to breast cancer cell development.
- Hepatocyte growth factor stimulates 5alpha-R1 expression through up-regulation of the transcription factor early growth response 1
- Data demonstrate that a decrease in the level of Egr-1, one of the targets for MAPK, by Tat has a negative impact on the level of p35 expression in NGF-treated neural cells.
- Transcriptional activation of the EGR-1 promoter by JNK-MKK7 or by IL-1 required a single upstream AP-1 site and three distal serum-response elements (SRE).
- CAR requires early growth response 1 to activate the human cytochrome P450 2B6 gene
- ERK and JNK MAPK/Elk-1/Egr-1 signal cascade is required for p53-independent transcriptional activation of p21(Waf1/Cip1) in response to curcumin in U-87MG human glioblastoma cells
- APE1 regulated PTEN expression is mediated by Egr-1.
- ATP activated MAPKs through the P2Y2 purinoceptor/PLC/PKC/ERK signaling pathway and induced translocation of ERK1/2 into the nucleus.
- EGR1 gene is implicated in idiopathic absence epilepsy.
- Egr-1 plays an important regulatory role in the transcriptional activation of hTERT.
- Activation of BAG3 by Egr-1 in response to FGF-2 in neuroblastoma.
- After high glucose stimulation, AhR was found in complex with Egr-1 & activator protein-2.
- Sp1/Egr1-repeat polymorphism in the ALOX5 promoter is not a genetic marker for the risk of developing late-onset Alzheimer disease
- EGR1 is an important transcriptional stimulator of the human PCYT2 and that conditions that modify EGR1 also affect the function of ECT and consequently PE synthesis
- the basal activity of the human PRDM1 promoter, through which several factors, including SP1, SP3 and early growth response 1, modulate its expression through a conserved GC-box.
- No pathological mutations were detected upon sequencing of the coding regions and the adjacent intronic regions of EGR1 in 96 unrelated Chinese subjects with high myopia.
- The role of the Early Growth Response-1 transcription factor in TNFalpha autoregulation in HaCaT human keratinocytes was investigated.
- Histamine-induced Egr-1 expression is dependent on the activation of the H1 receptor, and rapidly and transiently activates PKCdelta, ERK1/2, p38 kinase, and JNK prior to Egr-1 induction.
- DIDS-sensitive Cl- channels play a key role in the Low-density lipoprotein-induced cell proliferation of human aortic smooth muscle cells via the activation of Erk1/2 and PI3K/Akt and the upregulation of Egr-1.
- These findings define a Smad-independent TGF-beta signal transduction mechanism that underlies the stimulation of Egr-1.
- growth factors and serum induce expression of Egr-1 and SRF, respectively, which in turn induces E2-EPF UCP expression that positively regulates cancer cell growth
- demonstrate a critical role for Egr-1 in promoting autophagy and apoptosis in response to cigarette smoke exposure in vitro and in vivo
- PGE(2) via EP(4) activates the EGFR --> ERK1/2 --> Egr-1 pathway, leading to increased Id-1 transcription and cell invasion
- Report phorbol 12-myristate 13-acetate responsive sequence in Galphaq promoter during megakaryocytic differentiation. Regulation by EGR-1 and MAP kinase pathway.
- Results indicate that M12 cells undergo Egr1-dependent apoptotic response upon UV stimulation and led to the identification of downstream targets of Egr1, which mediate epidermal growth factor receptor function.
- we show a strong association between endoglin and EGR-1, increased collagen and Ssmooth muscle cells expression, decreased levels of intraplaque thrombosis
- activation of ESE-1 via enhanced nuclear translocation mediates tolfenamic acid-induced EGR-1 expression, which plays a critical role in the activation of apoptosis
- Early growth response-1 regulates angiopoietin-1-induced endothelial cell proliferation, migration, and differentiation.
- HDAC4 contributes to transcriptional induction of mPGES-1 by IL-1 through a mechanism involving up-regulation of Egr-1 transcriptional activity
- thalidomide could suppress leukemia cell invasion and migration by upregulation of Egr-1
- The present study demonstrates the differential up-regulation of Egr-1 in the thrombus-covered wall of human Abdominal Aortic Aneurysm (AAA) and also suggests its possible contribution to the thrombogenic and inflammatory pathogenesis in human AAA.