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Validated All-in-One™ qPCR Primer for DLG4(NM_001365.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- PSD-95 has a role in regulating the functional activity and intracellular trafficking of 5-HT2A receptors and possibly other GPCRs
- A single nucleotide polymorphism was identified as not significantly associated with schizophrenia.
- PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current
- ApoEr2 can form a multiprotein complex with NMDA receptor subunits and PSD95
- it is unlikely that the PSD-95 polymorphisms investigated play a substantial role in conferring susceptibility to schizophrenia in the Chinese population
- GABARAP and DLG4 genes are involved in the etiology of nicotine dependence in European-American smokers.
- thermodynamic parameters associated with the binding of several series of linear peptides to the third PDZ domain of PSD-9 have been measured using isothermal titration calorimetry
- validated occurrence of an unusual TG 3' splice site in intron 5
- PSD-95, the Kv1.3 potassium channel, and insulin receptor serine kinase co-localize to regulate membrane excitability and synaptic transmission at critical locations in the olfactory bulb.
- PSD-95 levels increased postnatally to reach a stable plateau by early childhood with a slight reduction in late adolescence and early adulthood
- Increase in postsynaptic density protein PSD-95 expression positively correlates with beta amyloid and phosphorylated Tau proteins in Alzheimer's disease cases.
- Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions.