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Validated All-in-One™ qPCR Primer for NQO1(NM_000903.2) Search again
Product ID:
HQP004317
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
DHQU, DIA4, DTD, NMOR1, NMORI, QR1
Gene Description:
NAD(P)H quinone dehydrogenase 1
Target Gene Accession:
NM_000903.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase.
Gene References into function
- polymorphisms associated with idiopathic Parkinson's disease
- Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1.
- Lack of NQO1 induction in human tumors is not due to changes in promoter region.
- Site-directed mutagenesis of cysteine to serine in the DNA binding region of Nrf2 decreases its capacity to upregulate antioxidant response element-mediated expression and antioxidant induction of NAD(P)H:quinone oxidoreductase1 gene
- Glutathione S-transferase P1 and NADPH quinone oxidoreductase polymorphisms are associated with aberrant promoter methylation of P16(INK4a) and O(6)-methylguanine-DNA methyltransferase in sputum.
- NQO1-Pro/Pro genotype is a risk factor for lung adenocarcinoma development
- A novel plasma membrane quinone reductase and NAD(P)H:quinone oxidoreductase 1 are upregulated by serum withdrawal in human promyelocytic HL-60 cells as a model to analyze cell responses to oxidative stress.
- The 465 SNP was the major cause of increased alternative splicing and decreased expression of NQO1 protein in HCT-116R30A cells.
- NQO1 regulates p53 proteasomal degradation independently of MDM2 and ubiquitin.
- laminar flow-induced promoter activation in endothelial cells is inhibited by expression of antisense Nrf2
- Benzo(a)pyrene activates extracellular signal-related and p38 mitogen-activated protein kinases in HT29 colon adenocarcinoma cells: involvement in NAD(P)H:quinone reductase activity and cell proliferation
- The genotype distribution of the NQO1 gene does not indicate a role for base excision repair in the development of therapy-related acute myeloblastic leukemia
- inactivating NQO1 polymorphism is associated with an increased risk of de novo leukemia with MLL translocations in infants and children
- association between p53 and NQO1 that may represent an alternate mechanism of p53 stabilization by NQO1 in a wide variety of human cell types.
- the NQO1 genetic polymorphism elevates bladder cancer risk, especially in male Caucasian smokers
- NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism is associated with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population
- CYP2E1 and NQO1 genotypes may play an important role in development of smoking related bladder cancer among Korean men
- "...NQO1 expression is up-regulated by B[a]P treatment." P. 1040
- These results provide the first evidence of a proximal repressor region exerting a negative role on the regulation of the hNQO1 promoter in small cell lung carcinoma.
- NQO1 has an important role in stabilizing hot-spot p53 mutant proteins in cancer
- This experiment conclude that NQO1 activity co-localizes closely with AD pathology supporting a presumed role as an antioxidant system upregulated in response to the oxidative stress of the AD process.
- The subjects carrying NQO1c. 609 T/T genotype and together with the habit of smoking or drinking may be more susceptible to benzene poisoning.
- NO signals the transcriptional up-regulation of NQO1 and other detoxifying enzyme and protective genes through Nrf2 via the ARE to counteract NO-induced apoptosis of neuroblastoma cells
- NQO1 gene polymorphism may confer susceptibility to the development of tardive dyskinesia in schizophrenia
- In smokers the variant NQO1 genotype may confer an increased risk for squamous cell carcinoma.
- The association between oral contraceptives use and breast cancer risk is dependent on NQO1 genotype, age and menopausal status.
- While overt NQO1 immunoreactivity was absent in the surrounding nervous tissue, in the Parkinsonian SNpc a marked increase in the astroglial and neuronal expression of NQO1 was consistently observed.
- NQO1 may have a role in familial acute myeloid leukemia
- Nrf3 is a negative regulator of ARE-mediated gene expression of NQO1
- The observed risk reductions of lung cancer may be attributable to the greatly reduced procarcinogen activating of NAD(P)H:quinone oxidoreductase 1 in individuals with at least one copy of the T allele.
- There may be a modulating role for NQO1 in the pathogenesis of pediatric sporadic Burkitt's lymphoma.
- Possible role for NQO1 gene as an MLL-independent risk factor, in the leukemogenic process of this subtype of infant acute lymphoblastic leukemia.
- The joint carriage of CYP1A1 Val(462) and NQO1 Ser(187) alleles, particularly in smokers, was related to colorectal adenoma risk, with a propensity for formation of multiple lesions
- Bach1 competes with Nrf2 leading to negative regulation of the antioxidant response element (ARE)-mediated NAD(P)H:quinone oxidoreductase 1 gene expression and induction in response to antioxidants
- NQO1 has a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome
- NQO1 transcription might be inappropriately suppressed by promoter hypermethylation in a subset of hepatocellular carcinoma, as well as GSTP1 gene
- Immunohistochemistry of resected pancreatic specimens demonstrated an increased immunoreactivity for NQO1 in pancreatic cancer
- Low/null activity polymorphisms of this enzyme is not with the risk of developing aplastic anemia in Caucasian patients.
- The increased expression in noncancer pancreatic tissue from smokers suggests that NQO1 expression may be a good candidate as a biomarker for pancreatic cancer, especially in risk groups such as smokers.
- NAD(P)H:quinone acceptor oxidoreductase (NQO) gene family belongs to the flavoprotein clan and, in the human genome, consists of two genes (NQO1 and NQO2).(review)
- Up-regulation of NQO1 may represent an adaptive stress response to limit further disease progression by detoxifying reactive species.
- In Israeli patients, NQO1*2 does not predispose to de novo AML; analysis showed significant differences in the frequencies of NQO1*2 by ethnic group
- transfection of SK-N-MC cells with NQO1 protects against dopamine-induced toxicity
- From in silico docking and comparative analysis, novel inhibitors of human NAD(P)H quinone oxidoreductase (NQO1) have been identified.
- Our findings suggested that this polymorphism might not represent additional genetic risk factor for LOAD.
- NQO1 gene polymorphisms is associated with sporadic distal colorectal adenomas
- NQO1 polymorphism confers interindividual variability of response to treatment in patients with acute myeloid leukemia
- the NQO1 C609T single-nucleotide polymorphism is associated with approximately 7-fold lower NQO1 activity.
- This is the first study showing robust (to bias due to population structure) evidence that the NQO1 C609T variant is a risk factor for childhood leukemia.
- Our preliminary report confirmed in multiple myeloma patients the trend, for a worse response to therapy in patients positive for NQO1*2.
- NQO1 has an important role as a mediator of neutrophil elastase-regulated oxidant stress and MUC5AC mucin gene expression.
- role for NQO1 in the metabolic complications of human obesity
- Data are consistent with the notion that NQO1 polymorphisms influence the course and clinical outcomes of urinary bladder neoplasms.
- The chemopreventive activity of taxifolin was determined by measuring the activity of NQO1 in HCT116 cells.
- NQO1 TT genotype may offer protection from reflux complications such as Barrett esophagus and esophageal adenocarcinoma.
- Overexpression of NQO1 in various tumors suggest the feasibility of developing diaminophenothiazinium-based redox cyclers into anticancer agents.
- NQO1 polymorphism is associated with urothelial cancer
- Case-control study of the possible relationship between the NQO1 gene polymorphism and mood disorders (patients with major depressive disorder, patients with bipolar I disorder, controls was carried out using PCR-based techniques.
- down-regulation of NQO1 effectively suppresses TNF-alpha-induced human aortic smooth muscle cell migration through inhibition of MMP-9 expression
- potentially higher levels of iron-generated oxidative stress related gene alleles may be at increased risk of breast cancer
- The highly expressed and inducible endogenous NQO1 in cardiovascular cells may act as a potential O2(.-) scavenger.
- Patients carrying a variant low-activity NQO1 allele had a significantly increased risk of developing a SMN. The observed effect was restricted to solid tumors
- NQO1 protein genotype was not associated with warfarin dose requirements in the African-Americans population.
- Polymorphisms in NQO1 is not associated with multiple myeloma
- The NQO1 609CT genotype is associated with increased adenoma risk among smokers, not diminished by high fruit and vegetable consumption. The observed gene-gene interactions may point to a role for NFE2L2 polymorphisms in NQO1-related adenoma formation
- study shows that NQO1 is consistently overexpressed in pancreatic ductal adenocarcinoma and may be a clinically useful diagnostic adjunct for detection of ancreatic ductal adenocarcinoma
- Non-laminar flow during cardiopulmonary bypass may diminish the transcriptional activation of the NQO1 in T carriers
- gene polymorphisms in CYP1A1, GSTT1, GSTP1, GSTM1, and NQO1 were characterized in Saudi individuals with a diagnosis of DLBCL; CYP1A1, GSTT1, GSTP1 demonstrated significant association of DLBCL risk; GSTM1 and NQO1 did not.
- Benzene exposed workers with the T/T genotype for NQO1 (NAD(P)H dehydrogenase quinone 1) showed significant increases in Micronuclei and Chromosomal aberrations
- The researchers found an association between the NQO1 homozygous wild-type allele and reduced birth weight, birth length, and birth head circumference in babies born to smoking mothers.
- Study of Turkish prostate cancer patients suggests that mutation of the NQO1 gene may effect the serum PSA and alkaline phosphatase levels.
- These results indicated that the C/C genotype had a possible protective effect against AD development, and the T allele might be a weak risk factor for late onset AD.
- Data show that p33(ING1b) is degraded in the 20S proteasome and that NAD(P)H quinone oxidoreductase 1 (NQO1), previously shown to modulate the degradation of p53 in the 20S proteasome, inhibits the degradation of p33(ING1b).
- chlorophyllin exerts antioxidant effect by inducing HO-1 and NQO1 expression mediated by PI3K/Akt and Nrf2
- ATBF1 and NQO1 as candidate targets for allelic loss at chromosome arm 16q in breast cancer: absence of somatic ATBF1 mutations and no role for the C609T NQO1 polymorphism.
- study concluded that genetic variation, especially the NQO1 609C > T polymorphism, is a more important predictor of rectal NQO1 phenotype than fruit and vegetable consumption
- Analyses indicated no association between the NQO1*2 polymorphism and the risk of anthracycline-related CHF
- NQO1 genotype is a prognostic and predictive marker for breast cancer.
- Significantly different distribution of the HO-1 genotype was found in subjects with different-stage UCs; however, it was not related to the NAD(P)H:quinone oxidoreductase 1 genotype.
- genetic polymorphisms for NQO1, CYP2E1, and ALDH2 synergistically with cumulative smoking amounts and alcohol drinking levels interact in the carcinogenesis of lung cancer in Koreans.
- alpha-lipoic acid controls cellular redox status, and upregulates quinone reductase NQO1 via Nrf2 activation in human leukemia HL-60 cells
- The combined genotypes of T/T in NQO1 Pro187Ser and Val/Val in MnSOD Ala-9Val polymorphisms were found to be independently associated with a significantly higher risk of TD.
- LPS induces NQO1 and HO-1 expression in human monocytes via Nrf2 to modulate their inflammatory responsiveness
- in Burkitt's lymphoma cell lines the NQO1 gene is not efficiently translated and this effect is not related to (C609T) polymorphism.
- Resveratrol can prevent breast cancer initiation by blocking multiple sites in the estrogen genotoxicity pathway.