Validated All-in-One™ qPCR Primer for ASXL1(NM_015338.5) Search again

Product ID:

HQP004287 (click here to view gene annotation page)

Species:

Human

Symbol:

ASXL1

Alias:

BOPS, MDS

Gene Description:

ASXL transcriptional regulator 1

Target Gene Accession:

NM_015338.5(click here to view gene page)

Estimated Delivery:

Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.

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Important Note:

By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.

Catalog#	Description	Note	Price (US$)
HQP004287	All-in-One™ qPCR Primer for NM_015338.5 (200 reactions)	ASXL1	inquire
HQP006940	All-in-One™ qPCR Primer for NM_002046.6 (200 reactions)	Reference Gene GAPDH	inquire
HQP006940	All-in-One™ qPCR Primer for NM_002046.7 (200 reactions)	Reference Gene GAPDH	inquire
HQP015171	All-in-One™ qPCR Primer for NM_004048.3 (200 reactions)	Reference Gene B2M	inquire
HQP015171	All-in-One™ qPCR Primer for NM_004048.4 (200 reactions)	Reference Gene B2M	inquire
HQP016381	All-in-One™ qPCR Primer for NM_001101.5 (200 reactions)	Reference Gene ACTB	inquire

Validated result:


A	B
Figure: All-in-One™ qPCR Primer Validated result to ASXL1. Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

In Drosophila, the Additional sex combs (Asx) gene encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. Although the function of the protein encoded by this gene is not known, it does show some sequence similarity to the protein encoded by the Drosophila Asx gene.