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Validated All-in-One™ qPCR Primer for SLC30A8(NM_173851.2) Search again
Product ID:
HQP004229
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ZNT8, ZnT-8
Gene Description:
solute carrier family 30 member 8
Target Gene Accession:
NM_173851.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A8, permit cellular efflux of zinc (Seve et al., 2004 [PubMed 15154973]).[supplied by OMIM].
Gene References into function
- identified the full-length sequences of SLC30A8, extending the SLC30 family to ten members; used an expressed sequence tag (EST) data mining strategy to determine the pattern of ZnT genes expression in tissues
- ZnT-8 may be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.
- ZnT-8 is a pancreatic beta-cell-specific zinc transporter [review]
- A non-synonymous polymorphism in SLC30A8 (rs13266634, R325W) is associated with increased risk of developing type 2 diabetes in causcasians.
- Rare mutaations are unlikely to be responsible for maturity onset diabetes of the young or other forms of early onset type 2 diabetes.
- The zinc transporter ZnT8 (Slc30A8), was targeted by autoantibodies in 60-80% of new-onset Type 1 diabetes (T1D) compared with <2% of controls and <3% type 2 diabetic and in up to 30% of patients with other autoimmune disorders with a T1D association.
- For the first time, the expression of hZnT-8 is shown in peripheral blood lymphocytes. It varied strongly between individuals.
- The association of 6 loci with type 2 diabetes risk in Japanese patients is reported.
- SLC30A8 rs13266634 gene variation is associated with protection from the development of posttransplantation diabetes mellitus in renal allograft recipients.
- Impaired insulin secretion is specifically present in impaired proinsulin conversion is specifically present in a variant of SLC30A8.
- Of European non-diabetic offspring of type 2 diabetes patients, 46% are homozygous carriers of the Arg325Trp polymorphism of SLC30A8.
- A non-synonymous variant in SLC30A8 is not associated with type 1 diabetes.
- Little evidence of association was observed between SNPs in SLC30A8 and type 2 diabetes in African Americans.
- Data confirmed the associations of single nucleotide polymorphisms in SLC30A8 with risk for type 2 diabetes in Asians.
- Data show that a common nonsynonymous single nucleotide polymorphism in the SLC30A8 gene determines ZnT8 autoantibody specificity in type 1 diabetes.
- SLC30A8 is a susceptible locus for type 2 diabetes in Chinese population, and its variant can influence insulin secretion.
- The results indicate that in Chinese Hans, common variants in SLC30A8 loci independently or additively contribute to type 2 diabetes risk, likely mediated through beta-cell dysfunction.
- Study show that polymorphisms in SLC30A8 were associated with type 2 diabetes risk in the studied population.
- variant residue at amino acid 325 is a key determinant of humoral autoreactivity to ZnT8 and that the SLC30A8 genotype is an important determinant of autoantibody specificity
- Single nucleotide polymorphism in SLC30A8 is associated with type 2 diabetes.
- The C-terminal domain of human ZnT8 contains at least two discrete epitopes, one of which is critically dependent upon the arginine residue at position 325.
- SLC30A8 provides an important additional and independent predictive marker for T1 diabetes mellitus.