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Validated All-in-One™ qPCR Primer for CYP2E1(NM_000773.3) Search again
Product ID:
HQP003817
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CPE1, CYP2E, P450-J, P450C2E
Gene Description:
cytochrome P450 family 2 subfamily E member 1
Target Gene Accession:
NM_000773.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
Gene References into function
- There is a correlation between the RsaI polymorphism homozygous uncut genotype in the CYP2E1 gene and a higher relative risk of nasopharyngeal carcinoma development in the Thai or Chinese populations in Thailand.
- increased translation of collagen mRNA by CYP2E1-derived reactive oxygen species is responsible for the increase in collagen protein
- Damage to mitochondria may be a critical step for cellular toxicity by CYP2E1-derived reactive oxygen species
- CYP2A6/2A7 and CYP2E1 expression in human oesophageal mucosa: regional and inter-individual variation in expression and relevance to nitrosamine metabolism
- Individuals possessing more susceptible CYP2E1 c2c2 genotypes were more likely to reveal p53 overexpression. Susceptible CYP2E1 genotypes may modulate the mutation of the p53 gene among VCM-exposed workers.
- In the CYP2E1 gene 5'-flanking region polymorphism, patients with esophageal cancer showed significantly higher frequency of the A4/A4 genotype compared with the control subjects (p = 0.02), but no difference was found in patients with lung cancer.
- expression of human cytochrome P450 2E1 gene in embryonic nasopharynx, nasopharyngeal cancer cell lines and tissue
- Overexpression of this enzyme overexpression up-regulates both non-specific delta-aminolevulinate synthase and heme oxygenase-1 in a human hepatoma cell line.
- substitution of residue 363 in cyp2e1 resulted in significant alterations of the metabolite profile for the side chain hydroxylation of 7-butoxycoumarin
- The CYP2E1 variant genotype did not significantly increase the risk for neoplasia.
- Polymorphisms of alcohol-metabolizing enzymes: analyses of mutations on the CYP2E1, ADH2, ADH3 and ALDH2 genes in a Mexican-American population living in the Los Angeles area.
- CYP2E1 genetic polymorphism may be associated with susceptibility to breast cancer in alcohol-consuming women.
- hepatic cytochrome P450 2E1 activity and lymphocyte cytochrome P450 2E1 expression are enhanced in nondiabetic nonalcoholic steatohepatitis
- CYP 2E1 genetic polymorphism may be associated with susceptibility to antituberculosis drug-induced hepatitis.
- This study shown that the genotypes of CYP2E1 are associated with clinical features of alcoholics.
- Down-regulation of cytochrome P450 CYP2E1 is associated with breast cancer
- Proteasome activity plays an important role in modulating CYP2E1-mediated toxicity in HepG2 cells by regulating CYP2E1 levels and by removal of oxidized proteins.
- HO-1 induction was observed in the livers of chronic alcohol-fed mice or pyrazole-treated rats, conditions known to elevate CYP2E1 levels.
- P450 2E1 has a role in inducing heme oxygenase-1 through ERK MAPK pathway
- association of CYP2E1*1D with alcohol and nicotine dependence suggests that CYP2E1 may contribute to the development of these dependencies
- CYP2E1 and NQO1 genotypes may play an important role in development of smoking related bladder cancer among Korean men
- CYP2E1 has a role in oxidative stress causing mitochondrial damage along with phospholipase A2
- Arg76 is closely associated with the function of CYP2E1, and that the genetic polymorphism of cytochrome p450 2E1 is one cause of interindividual differences in the toxicity of xenobiotics
- These results show that CYP2E1 protein is expressed in both tumour and normal breast tissue with an increased expression in breast tumours.
- hepatic CYP2E1 activity is up-regulated in morbidly obese subjects
- Transcriptional activity of the mutant allele of the tandem repeat polymorphism in the 5'-flanking region of the CYP2E1 gene is greater than that of the wild type.
- CYP2E1 was clearly expressed in human fetal liver.
- Levels of CYP2E1 are elevated under a variety of physiological and pathophysiological conditions, and after acute and chronic alcohol treatment.
- biochemical and toxicological properties of CYP2E1(review)
- The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver.
- The cDNA of human CYP2E1 can be successfully cloned, and a cell line, HepG2-CYP2E1 can efficiently express mRNA and has CYP2E1 activity.
- enhanced injury in hepatocytes over expressing both Hepatitis C virus core protein and CYP2E1 is mediated by increases in oxidative stress.
- CYP2E1 polymorphisms are associated with incomplete intestinal metaplasia in a high-risk area of stomach cancer
- The objective of this study was to analyze the effect of the green tea flavanol epigallocatechin-3-gallate (EGCG), which has been shown to prevent alcohol-induced liver damage, on CYP2E1-mediated toxicity in HepG2 cells overexpressing CYP2E1 (E47 cells).
- CYP2E1 is a unique gene expressing in liver but did not express in hepatocellular carcinoma
- polymorphism and susceptibility to cirrhosis or pancreatitis in alcoholics
- marked impairment of CYP enzyme activity during allograft rejection which is presumably secondary to an increased intragraft production of proinflammatory cytokines and NO.
- overexpression of human CYP2E1 activates acetaminophen to reactive metabolites which damage mitochondria, form protein adducts, and result in toxicity to HepG2 cells
- increased hepatocyte CYP2E1 expression and the presence of steatohepatitis result in the down-regulation of insulin signaling
- The aim of this study was to determine whether hepatitis C virus core protein and alcohol-inducible cytochrome P450 2E1 contribute to reactive oxygen species production and cytotoxicity in human hepatoma cells.
- Nicotinamide adenine dinucleotide phosphate oxidase-derived oxidants are critical for development of ethanol-induced liver injury. CYP2E1 is required for induction of oxidative stress to DNA and may play role in ethanol-associated hepatocarcinogenesis.
- To address this question, we determined whether DLPC protects against alcohol-induced cytotoxicity in HepG2 cells expressing CYP2E1.
- a decrease of Hsp60 in the cytoplasmic fraction of dilated cardiomyopathy-affected left ventricles was observed; at the same time an increase in P450 2E1 expression in dilated hearts' cytoplasmic fractions was observed
- the CYP2E1 genotype may influence individual susceptibility to development of gastric cardia cancer, and that the risk increases significantly in smokers.
- Polymorphisms of the alcohol metabolism-related CYP2E1 gene are significantly different in Korean patients with alcoholism and Korean control subjects without alcoholism.
- The CYP2E1 genotype frequencies are 66.7% for type of c1/c1, 30% for type of c1/c2, 3.3% for type of c2/c2 with the allele frequencies 0.818 for C1 and 0.182 for C2, and the gene distribution matched the equilibrium law of Hardy-Weinberg.
- CYP2E1 priming could explain the sensitization of Kupffer cells to LPS activation by ethanol, a critical early step in alcoholic liver disease
- CYP2E1 wild type (c1/c1) increased the susceptibility to esophageal squamous cell cancer risk in Kazakh individuals with GSTM1 presence genotype
- Low concentrations of iron and arachidonic acid synergistically interacted with CYP2E1 to produce cell toxicity, suggesting these nutrients may act as priming or sensitizing agents to alcohol-induced liver injury.
- A positive relationship between ethanol-induced oxidative damage in human primary cultured hepatocytes and CYP2E1 activity was exhibited
- A PCR method for identifying polymorphism.
- the A4/A4 genotype of the 5'-flanking region of CYP2E1 has a role in development of non-small cell lung carcinoma in Japanese patients
- Protein concentration of CYP2E1 is significantly lower by ~81% in the sigmoid colon compared to the level in the descending colon.
- We report here for the first time three novel alternative spliced mRNA transcripts which are more frequently present in lung carcinoma cell lines as in hepatocyte cell lines.
- Potentiation of serum deprivation-induced cell death by CYP2E1 may contribute to the sensitivity of the liver to alcohol-induced ischemia and growth factor deprivation.
- CYP2E1 has a role in sensitizing cells to the toxicity caused by depletion of glutathione
- frequencies of this high-activity polymorphism in alcohol related patient groups
- These results suggest that CYP2E1 mediated oxidative stress downregulates the expression of GRP proteins in HepG2 cells and oxidative stress is an important mechanism in causing ER dysfunction in these cells.
- Nrf2, through up-regulation of glutamate-cysteine ligase and increase of GSH levels, protects against CYP2E1-dependent AA toxicity
- studies suggest that the frequency of the c2 alleles in the Polish population is low; it seems, however, that they pose the risk of alcoholic cirrhosis
- CYP2E1-b(5) complex model was constructed, leading to improved insights into the protein interaction.
- findings indicated a gene-gene interaction between CYP2E1 and GSTM1 was accessible to developing breast cancer in Taiwanese women without the habits of cigarette smoking and alcohol consumption
- increased toxic interactions by TGF-beta1 plus CYP2E1 can occur by a mechanism involving increased production of intracellular ROS and depletion of GSH, resulting in mitochondrial membrane damage and loss of membrane potential, followed by apoptosis
- Expression of Thy1 decreased in differentiated ADSC and BMSC. Expression of albumin, CYP2E1, and CYP3A4 increased in differentiated BMSC and ADSC. Hepatic gene activation may involve increased C/EBPbeta and HNF4alpha.
- Ethanol induced CYP2E1 generates oxidative stress that is responsible for the decrease in proteasome activity.
- This report is the first demonstration that thalassemia major is associated with an alteration of CYP2E1 and CYP3A4 activities; this could modify the sensitivity of thalassemia patients to the toxic or therapeutic effects of drugs.
- This documents the presence of a polymorphism of CYP2E1 that is overexpressed in alcoholic Otomies, in which the variant allele (A1 of CYP2E1/TaqI) is associated with increased susceptibility to alcoholism.
- The expression of CYP2E1 in Nasopharyngeal carcinoma (NPC) cells, embryonic nasopharyngeal epithelial tissue (ENET) specimens, and NPC biopsies was analysed.
- comparison of substrate dynamics in CYP2E1 and CYP2A6
- The unusually rapid carbon monoxide binding kinetics of P450 2E1 indicate that it is more dynamically mobile than other P450s and thus able to more readily interconvert among alternate conformations.
- The frequency of the CYP2E1 allele distribution was found to be markedly different between Chinese and South African populations.
- Induction of DNA strand breaks and oxidative stress in HeLa cells by ethanol is dependent on CYP2E1 expression.
- The aim of this study was to determine the role of these polymorphisms for the transcriptional regulation of the human CYP2E1 gene in general transcription and during enzyme induction
- Differences exist in protein levels of certain CYPs in non-malignant esophageal tissue (e.g. CYP2C8, CYP3A4, CYP3A5, and CYP2E1) between SCC patients and healthy subjects and may contribute to the development of squamous-cell carcinoma in the esophagus.
- The genotype frequencies of CYP2E1*7B in Turkish population were found to be similar to those of other Caucasian populations
- Our studies suggest that the frequency of allele c2 in Polish population is low, but the presence of c2 allele may be a risk factor for the alcohol liver cirrhosis.
- the level of CYP2E1 is induced by hypertonic condition via TonEBP transactivation. The present study suggests that osmotic status may influence individual responses to the substrate of CYP2E1.
- The CYP2E1 and NAT2 variants associated with COPD
- examined genetic polymorphisms in the alcohol dehydrogenase 3, aldehyde dehydrogenase 2, and cytochrome P450 2E1 genes in 505 patients with histologically confirmed lung cancer and 256 noncancer controls to determine association with alcohol drinking
- Gastric cancer in patients younger than 40 years is closely associated with H. pylori infection, but not with genetic characteristics.
- Chlorzoxazone metabolism in vivo remains the only available method for CYP2E1 phenotyping.
- CYP2E1 polymorphism is not associated with the risk of head and neck cancer
- CYP2E1 activity cannot be used to distinguish nonalcoholic steatotis patients from steatohepatitis patients.
- Genetic polymorphism in CYP2E1 may be responsible for individual differences in susceptibility to liver fibrosis with regard to chronic vinyl chloride monomer exposure.
- Findings suggest that human and cynomolgus monkey CYP2E1 enzymes have high homology in their amino acid sequences, and that their enzymatic properties are considerably similar.
- The CYP2E1 c2/c2 genotype increases susceptibility to rectal cancer and the gene-environmental interactions between the CYP2E1 polymorphism and smoking or alcohol drinking exist for colorectal neoplasia in general.
- Individuals carrying the *5A or *6 alleles of CYP2E1 are associated with gastric cancer in drinkers
- Individuals carrying combinations of CYP2E1*5B, *6 and *7B variants together are likely associated with the risk of developing childhood ALL.
- carrying c2 allele and DD genotype of CYP2E1 conferreded an elevated risk for esophageal squamous cell carcinoma.
- The H6, H7, and H9 haplotypes may play certain roles in different clinical phenotypes in Mexican American alcoholics. In addition, our data suggest that the H1, H2, and H3 haplotypes are associated with alcohol drinking and smoking.
- biochemical analysis of CYP2E1 substrate inhibition
- QSAR analysis for substrate specificity of six CYP isoforms, revealing that CYP2C9 substrates are anionic compounds, while CYP2D6 substrates are cationic, and CYP2E1 substrates are smaller compounds, while CYP3A4 substrates are larger compounds
- The researchers found an association between the CYP2E1 polymorphism and an increased risk of low birth weight infants in mothers who smoke.
- CYP2E1 RsaI polymorphism does not appear to contribute to the etiology of bladder cancer in a south-eastern Chinese population
- Alcohol consumption and polymorphisms in the CYP2E1, ADH1B and ALDH2 genes are important risk factors for esophageal squamous cell carcinoma in Chinese males living in Gansu Province, China.
- Describe HepG2 cell line which constitutively expresses the human CYP2E1 form.
- Human CYP2E1 mediates the formation of glycidamide from acrylamide.
- Significant gene-environment interactions observed for CYP2E1*1B and CYP2E1*6 polymorphic genotypes may confer a substantial risk for upper aerodigestive tract cancers among Indians.
- These results suggest that specific phospholipids can regulate the function of CYP2E1 by interaction with the enzyme including the N-terminal region(s).
- Overexpression of CYP2E1 enhances sensitivity of hepG2 cells to fas-mediated cytotoxicity.
- Variation in activity and inducibility of CYP2E1 contribute to the development of colorectal carcinogenesis.
- diverse patterns of haplotype variation found in the different populations we have studied show that ethnicity may be an important variable helping to explain inconsistencies that have been reported by association studies
- Logistic regression analysis demonstrated a statistically significant association between CYP2E1 mRNA expression levels and liver abnormalities in the VCM-exposed workers.
- genetic polymorphisms for NQO1, CYP2E1, and ALDH2 synergistically with cumulative smoking amounts and alcohol drinking levels interact in the carcinogenesis of lung cancer in Koreans.
- These results suggest that the functional roles of CYP1A2 and 2E1 can be switched by interaction with a specific anionic lysophospholipid in vivo.
- Variation in activity and inducibility of CYP2E1, in relation to alcohol or red meat intake, contributes to the development of colorectal cancer in Japan.