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Validated All-in-One™ qPCR Primer for CSF2RB(NM_000395.2) Search again
Product ID:
HQP003170
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD131, CDw131, IL3RB, IL5RB, SMDP5, betaGMR
Gene Description:
colony stimulating factor 2 receptor subunit beta
Target Gene Accession:
NM_000395.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq].
Gene References into function
- hGM-CSF activation of hGM/beta(c)with or w/o the hbeta(c) subunit, promoted primitive phenotype maintenance, indicating that the hGM Ralpha chain cytosolic domain is needed for differentiation mediated by the hGM Ralpha, beta(c) receptor complex.
- soluble forms of the GM-CSF receptor alpha chain and beta chain were produced and a novel mechanism of receptor assembly was demonstrated
- JAK2 activation mediates cross-talk between extracellular domain mutants of the beta-subunit of the GM-CSF receptor and EpoR
- Interleukin-3 binding to the human betac receptor involves functional epitopes formed by domains 1 and 4 of different protein chains
- A minor improvement in resolution has given a clearer indication of the position and stabilization of the key residues Tyr15, Phe79, Tyr347, His349, Ile350 and Tyr403 in the elbow region between domain 1 and domain 4 of the dimer-related molecule.
- These results suggest that the JAK1-JH7-3 domains are required for interleukin-3 receptor common beta subunit interaction and abolish wild type JAK1 and JAK2-mediated signaling.
- The dynamic role of the common signaling subunit beta c is to prevent IL5alpha and GM-CSF ligand-receptor complexes from rapidly dissociating.
- The interaction between intercellular adhesion molecule 1 (ICAM-1) and the GM-CSF receptor is essential for GM-CSF-induced eosinophil activation and survival.
- IL-5-induced effects on betac assembly in the presence of nontagged IL-5Ralpha provide direct evidence that IL-5 can cause higher order rearrangements of betac homo-oligomers
- Ligand-binding and receptor activation are not critically dependent on individual N-glycosylation sites within the IL-3- and IL-5-receptor common beta-subunit.